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In vitro clonal propagation of Achyranthes aspera L. and Achyranthes bidentata Blume using nodal explants

Asian Pacific Journal of Tropical Biomedicine 2012年第1期2卷 1-5页

作者： Wesely Edward Gnanaraj Johnson Marimuthu Mohanamathi RB Kavitha Marappampalyam Subramanian Department of Botany Arignar Anna Government Arts College Department of Plant Biology and Plant Biotechnology St. Xavier's College(Autonomous)Palayamkottai Centre for Biotechnology Research Department of BiotechnologyMuthayammal College of Arts & Science

Objective:To develop the reproducible in vitro propagation protocols for the medicinally important plants viz.,Achyranthes aspera(***)*** Achyranthes bidentata(***)Blume using nodal segments as ***:Young shoots of *** and *** were harvested and washed with running tap water and treated with 0.1%bavistin and rinsed twice with distilled *** the explants were surface sterilized with 0.1%(w/v)HgCl_2 solutions for I *** rinsing with sterile distilled water for 3-4 times,nodal segments were cut into smaller segments(1 cm)and used as the *** explants were placed horizontally as well as vertically on solid basal Murashige and Skoog(MS)medium supplemented with 3%sucrose,0.6%(w/v)agar(HiMedia,Mumbai)and different concentration and combination of 6-benzyl amino purine(BAP),kinetin(Kin),naphthalene acetic acid(NAA)and indole acetic acid(IAA)for direct ***:Adventitious proliferation was obtained from *** and *** nodal segments inoculated on MS basal medium with 3%sucrose and augmented with BAP and Kin with varied *** medium augmented with 3.0 mg/L of BAP showed the highest percentage(93.60±0.71)of shootlets formation for *** and(94.70±0.53)percentages for *** number of shoots/explants(10.60±0.36)for *** and(9.50±0.56)for *** was observed in MS medium fortified with 5.0 mg/L of *** ***,maximum mean length(5.50±0.34)of shootlets was obtained in MS medium augmented with 3.0 mg/L of Kin and for ***(5.40±0.61)was observed in the very same *** highest percentage,maximum number of rootlets/shootlet and mean length of rootlets were observed in 1/2 MS medium supplemented with 1.0 mg/L of *** percentages of plants were successfully established in *** eight percentages of plants were well established in the green house *** five percentages of plants were established in the ***:The

关键词： In vitro Plant regeneration Tissue culture Nodal culture Achyranthes aspera Achyranthes bidentata Clonal propagation Nodal explant Reproducible MS medium Shoots BAP NAA IAA Kinetin Nodal segment shootlet Rootlet

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