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Transcriptomic and proteomic analyses of far-red light effects in inducing shoot elongation in the presence or absence of paclobutrazol in Chinese pine

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Journal of Forestry Research 2022年第3期33卷 1033-1043页

作者： Yingtian Guo Shihui Niu Yousry A.El-Kassaby Wei Li National Engineering Laboratory for Forest Tree Breeding Beijing Advanced Innovation Center for Tree Breeding by Molecular DesignCollege of Biological Sciences and TechnologyBeijing Forestry UniversityBeijing100083People’s Republic of China Department of Forest and Conservation Sciences Faculty of ForestryUniversity of British Columbia2424 Main MallVancouverBCV6T 1Z4Canada

In angiosperms, low red (R)/far-red (FR) ratio light increases gibberellin (GA) levels. GA signaling in conifer seedlings requires FR to promote shoot elongation and reduce the inhibition of shoot elongation induced by paclobutrazol (PAC), yet the effects of far-red light in inducing shoot elongation in the presence or absence of PAC is poorly understood. In this study, transcriptomic and proteomic analyses was used to examine the molecular mechanism of FR regulation of shoot elongation in Chinese pine (Pinus tabuliformis Carr.) seedlings in the presence of PAC. Four treatments were compared: white light + water (WW), FR + water (RW), white light + PAC (WP), and FR + PAC (RP), and 1436 differentially expressed genes (DEGs) and 450 differentially expressed proteins (DEPs) were identified in RW_WW (RW contrast WW), and 1862 DEGs and 481 DEPs in RP_WP (RP contrast WP). Metabolic and signal transduction pathway analyses of DEGs and DEPs in RW_WW and RP_WP, indicated that the former required more energy than the latter. Moreover, gibberellic acid, auxin, and brassinolide are equally important in RW_WW and RP_WP for shoot elongation, except for the ethylene pathway. Amino acid metabolism and cell wall organization were significantly enriched in RW_WW and RP_WP, respectively. In summary, RW_WW and RP_WP had different effects in secondary metabolism, energy metabolism, amino acid metabolism, cell wall organization, and hormone response. These results provide an important theoretical and reference basis for studying the regulatory effect of low R/FR and PAC in conifer shoot elongation.

关键词： Low R/FR ratio Pinus tabuliformis Paclobutrazol Proteome shoot elongation Transcriptome

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Short-day treatment during the growing period limits shoot growth and increases frost hardiness of hybrid aspen plants in the nursery

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Forestry Studies in China 2007年第4期9卷 262-266页

作者： Zhang Gang Jaana Luoranen Heikki Smolander College of Horticulture Agricultural University of Hebei Baoding 071001 Hebei P. R. China The Finnish Forest Research Institute Suonenjoki Research Unit Juntintie 154 FIN-77600 Suonenjoki Finland

In Finland, under nursery conditions hybrid aspen may continue their shoot growth until early September. Thus, frost hardening is usually delayed. To solve this problem, we used a three-week period of short-day （SD） treatment between late July and mid-August. During autumn after frost exposure, frost hardiness （FH） was assessed three times with a stem-browning test. The resuits showed that after SD treatment shoot growth ceased and FH increased when compared to untreated hybrid aspen. Furthermore, the height of SD-treated hybrid aspen varied much less than that of the control plants. We conclude that SD treatment in the nursery during the growing period can be used as a supplementary method for producing well-hardened and uniform hybrid aspen plants.

关键词： frost hardiness nursery performance Populus shoot elongation short-day treatment

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维普期刊数据库

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Optimization of Agrobacterium-mediated transformation in soybean

Optimization of Agrobacterium-mediated transformation in soy...

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江苏省遗传学会2017年学术研讨会——“技术创新与遗传...

作者：李姝璇 Yahui Cong Yaping Liu Tingting Wang Qin Shuai Nana Chen Junyi Gai 李艳南京农业大学

High transformation efficiency is a prerequisite for study of gene function and molecular breeding. Agrobacterium tumefaciens-mediated transformation is a preferred method in many plants. However, the transformation efficiency in soybean is still low. The objective of this study is to optimize Agrobacterium-mediated transformation in soybean by improving the infection efficiency of Agrobacterium and regeneration efficiency of explants. Firstly, four factors affecting Agrobacterium infection efficiency were investigated by estimation of the rate of GUS transient expression in soybean cotyledonary explants, including Agrobacterium concentrations, soybean explants, Agrobacterium suspension medium, and co-cultivation time. The results showed that an infection efficiency of over 96% was achieved by collecting the Agrobacterium at a concentration of OD650 = 0.6, then using an Agrobacterium suspension medium containing 154.2 mg/L dithiothreitol to infect the halfseed cotyledonary explants（from mature seeds imbibed for one day）, and co-cultured them for five days. The Agrobacterium infection efficiencies for soybean varieties Jack Purple and Tianlong 1 were higher than the other six varieties. Secondly, the rates of shoot elongation were compared among six different concentration combinations of gibberellic acid（GA3） and indole-3-acetic acid（IAA）. The shoot elongation rate of 34% and 26% was achieved when using the combination of 1.0 mg/L GA3 and 0.1 mg/L IAA for Jack Purple and Tianlong 1, respectively. This rate was higher than the other five concentration combinations of GA3 and IAA, with an 18% and 11% increase over the original laboratory protocol（a combination of 0.5 mg/L GA3 and 0.1 mg/L IAA）, respectively. The transformation efficiency was 7% and 10% for Jack Purple and Tianlong 1 at this optimized hormone concentration combination, respectively, which was 2% and 6% higher than the original protocol, respectively. Finally, GUS histochemical staining, PCR, herbicide（glufosinate） painting, and QuickStix Kit for Lib

关键词： Agrobacterium efficiency half-seed explants infection regeneration shoot elongation soybean transformation

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