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Interaction between enteric epithelial cells and Peyer's patch lymphocytes in response to shigella lipopolysaccharide: Effect on nitric oxide and IL-6 release

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World Journal of Gastroenterology 2006年第24期12卷 3895-3900页

作者： Jie Chen Chuen-Pei Ng Dewi K Rowlands Peng-Hui Xu Jie-Ying Gao Yiu-Wa Chung Hsiao-Chang Chan Epithelial Cell Biology Research Center Department of Physiology Faculty of Medicine Chinese University of Hong Kong Hong Kong China Department of Immunology Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 China Department of Biology Faculty of Medicine ShanxiMedical University Taiyuan 030001 Shanxi Province China

AIM： TO investigate the effect of interaction between enteric epithelial cells and lymphocytes of Peyer＇s patch on the release of nitric oxide （NO） and IL-6 in response to shigella lipopolysaccharide （lps）. METHODS： Human colonic epithelial cells （Caco-2） were mixed cocultured with lymphocytes of Peyer＇s patch from wild-type （C57 mice） and inducible NO synthase knockout mice, and challenged with shigella f2a-12 lps. Release of NO and raiL-6 was measured by Griess colorimetric assay and enzyme-linked immunosorbent assay （ELISA）, respectively. RESULTS： In the absence of lps challenge, NO was detected in the culture medium of Caco-2 epithelial cells but not in lymphocytes of Peyer＇s patch, and the NO release was further up-regulated in both cocultures with lymphocytes from either the wild-type or iNOS knockout mice, with a significantly higher level observed in the coculture with iNOS knockout lymphocytes. After shigella f2a-12 lps challenge for 24-h, NO production was significantly increased in both Caco-2 alone and the coculture with lymphocytes of Peyer＇s patch from the wild-type mice but not from iNOS knockout mice. lps was found to stimulate the release of mIL-6 from lymphocytes, which was suppressed by coculture with Caco-2 epithelial cells. The lps-induced mIL-6 production in lymphocytes from iNOS knockout mice was significantly greater than that from the wild-type mice. CONCLUSION： Lymphocytes of Peyer＇s patch maintain a constitutive basal level of NO production from the enteric epithelial cell Caco-2. lps-induced mIL-6 release from lymphocytes of Peyer＇s patch is suppressed by the cocultured epithelial cells. While no changes are detectable in NO production in lymphocytes from both wild-type and iNOS knockout mice before and after lps challenge, NO from lymphocytes appears to play an inhibitory role in epithelial NO release and their own mIL-6 release in response to lps.

关键词： shigella f2a-12 lps Colon epithelial cells(Caco-2) Peyer's patch lymphocyte Coculture Nitricoxide Interleukin-6

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