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Reporter Gene Assay for Detection of shellfish Toxins

Biomedical and Environmental Sciences 2009年第5期22卷 419-422页

作者： WEI-DONG YANG MIN-YI WU JIE-SHENG LIU XI-CHUN PENG HONG-YE LI School of Life Science and Technology Jinan UniversityGuangzhou 510632GuangdongChina

Objective To explore the potential reporter gene assay for the detection of sodium channel-specific toxins in shellfish as an alternative for screening harmful algal bloom (HAB) toxins, considering the fact that the existing methods including HPLC and bioassay are inappropriate for identifying HAB toxins which poses a serious problem on human health and shellfish industry. Methods A reporter plasmid pEGFP-c-fos containing c-fos promoter and EGFP was constructed and transfected into T24 cells using LipofectAMINE 2000. Positive transfcctants were screened by G418 to produce a pEGFP-c-fos-T24 cell line. After addition of increasing neurotoxic shellfish poison (NSP) or GTX2,3, primary components of paralytic shellfish poison (PSP), changes in expression of EGFP in the cell line were observed under a laser scanning confocal microscope and quantified with Image-pro Plus software. Results Dose-dependent changes in the intensity of green fluorescence were observed for NSP in a range from 0 to 10 ng/mL and for GTX2,3 from 0 to 16 ng/mL. Conclusion pEGFP-c-fos-T24 can be applied in detecting HAB toxins, and cell-based assay can be used as an alternative for screening sodium channel-specific HAB toxins.

关键词： shellfish poisoning Green fluorescent protein Harmful algal bloom toxins

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Toxic Algae and Early Warning Management in Yellow Sea and Bohai Sea of China

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Animal Husbandry and Feed Science 2014年第2期6卷 83-86页

作者： Song Lun Song Guangjun Song Yonggang Xu Xiaohong Liaoning Ocean and Fisheries Science Research Institute Liaoning Key Laboratory of Marine Biological Resources and Ecology

The research status of toxic algae in Yellow Sea and Bohai Sea are reviewed from the aspects of toxicity characteristics,toxic mechanism and early warning management,and the existing toxic algae and their toxicity in Yellow Sea and Bohai Sea are analyzed in the paper. The early warning level of toxic algae in Yellow Sea and Bohai Sea of China is put forward,and the research direction of shellfish poisoning in future is summarized.

关键词： Toxic algae shellfish poisoning Early warning management Yellow Sea and Bohai Sea

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