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Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal

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Science China(Life Sciences) 2023年第5期66卷 1151-1169页

作者： Hong-Yan Wang Xiang Liu Jian-Yang Chen Yingyi Huang Yifang Lu Fujian Tan Qun Liu Mingming Yang Shuo Li Xianghui Zhang Yating Qin Wenxiu Ma Yingming Yang Liang Meng Kaiqiang Liu Qian Wang Guangyi Fan Rafael H.Nóbrega Shanshan Liu Francesc Piferrer Changwei Shao Key Lab of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural AffairsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266072China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China BGI Research-Qingdao BGIQingdao 266555China BGI-Shenzhen Shenzhen 518083China School of Marine Sciences Ningbo UniversityNingbo 315211China College of Marine Technology and Environment Dalian Ocean UniversityDalian 116023China Qingdao-Europe Advanced Institute for Life Sciences BGI-ShenzhenQingdao 266555China College of Life Sciences Shandong Normal UniversityJinan 250014China Reproductive and Molecular Biology Group Department of Structural and Functional BiologyInstitute of BiosciencesSão Paulo State University(UNESP)BotucatuSão PauloBrazil Institut de Ciències del Mar(ICM) Spanish National Research Council(CSIC)BarcelonaSpain

Female-to-male sex reversals(pseudomales)are common in lower vertebrates and have been found in natural populations,which is a concern under rapid changes in environmental *** can exhibit altered ***,the regulatory mechanisms underlying pseudomale spermatogenesis remain ***,we characterized spermatogenesis in Chinese tongue sole(Cynoglossus semilaevis),a species with genetic and environmental sex determination,based on a highresolution single-cell RNA-seq atlas of cells derived from the testes of genotypic males and *** identified five germ cell types and six somatic cell types and obtained a single-cell atlas of dynamic changes in gene expression during spermatogenesis in Chinese tongue sole,including alterations in *** detected decreased levels of Ca^(2+)signaling pathway-related genes in spermatogonia,insufficient meiotic initiation in spermatocytes,and a malfunction of somatic niche cells in ***,a cluster of CaSR genes and MAPK signaling factors were upregulated in undifferentiated spermatogonia of ***,we revealed that Z chromosome-specific genes,such as piwil2,dhx37,and ehmt1,were important for *** results improve our understanding of reproduction after female-to-male sex-reversal and provide new insights into the adaptability of reproductive strategies in lower vertebrates.

关键词： Cynoglossus semilaevis spermatogenesis single-cell transcriptome sex reversal spermatogonia

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ASER:Animal sex reversal Database

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Genomics, Proteomics & Bioinformatics 2021年第6期19卷 873-881页

作者： Yangyang Li Zonggui Chen Hairong Liu Qiming Li Xing Lin Shuhui Ji Rui Li Shaopeng Li Weiliang Fan Haiping Zhao Zuoyan Zhu Wei Hu Yu Zhou Daji Luo State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan 430072China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyInnovation Academy for Seed DesignChinese Academy of SciencesWuhan 430072China School of Basic Medical Science Wuhan UniversityWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Frontier Science Center for Immunology and Metabolism Wuhan UniversityWuhan 430072China

sex reversal,representing extraordinary sexual plasticity during the life cycle,not only triggers reproduction in animals but also affects reproductive and endocrine system-related diseases and cancers in *** reversal has been broadly reported in animals;however,an integrated resource hub of sex reversal information is still ***,we constructed a comprehensive database named ASER(Animal sex reversal)by integrating sex reversal-related data of 18 species from teleostei to *** systematically collected 40,018 published papers and mined the sex reversal-associated genes(SRGs),including their regulatory networks,from 1611 core *** annotated homologous genes and computed conservation scores for whole genomes across the 18 ***,we collected available RNA-seq datasets and investigated the expression dynamics of SRGs during sex reversal or sex determination *** addition,we manually annotated 550 in situ hybridization(ISH),fluorescence in situ hybridization(FISH),and im-munohistochemistry(IHC)images of SRGs from the literature and described their spatial expression in the ***,ASER provides a unique and integrated resource for researchers to query and reuse organized data to explore the mechanisms and applications of SRGs in animal breeding and human *** ASER database is publicly available at http://***/.

关键词： sex reversal sex reversal-associated gene Database Omics Conservation

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sex reversal and the androgenic gland (AG) in Macrobrachium rosenbergii: A review

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Aquaculture and Fisheries 2020年第6期5卷 283-288页

作者： Kianann Tan Huigong Jiang Donghuo Jiang Weimin Wang College of Fisheries Key Lab of Agricultural Animal GeneticsBreeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal BreedingMinistry of AgricultureHuazhong Agricultural UniversityWuhan430070China College of Natural and Applied Sciences University of GuamMangilaoGuam96913USA

The male giant freshwater prawn(Macrobrachium rosenbergii)generally exhibit faster growth rates compared to females of similar ***,the practice of culturing monosex(all-male)prawn can increase both the production yield and *** selecting males for culture during the grow-out period has long been practiced in commercial prawn farming,but is labor-intensive and generally unsuccessful in producing a male *** androgenic gland(AG)is essential for sexual differentiation in prawn and development of male primary and secondary sexual *** removal of the AG(andrectomy)from male prawn at an early development stage will transform the male into a fully-functioning *** *** androgenic gland-specific insulin-like hormone(Mr-IAG)plays critical roles in male spermatogenesis and male sexual *** interference(RNAi)is a highly specific post-transcriptional interference or silencing of gene expression in *** Mr-IAG had induced full and functional sex reversal of male to neo-female(genetic male with a female phenotype).Microsurgical removal of AG and RNAi are two different approaches to producing neo-females and both methods target the manipulation of AG for efficient ***-females can mate with normal males to produce all-male prawn progeny,which can be used for commercial prawn farming and to improve production efficiency and *** article reviewed recent research related to the development and functions of AG and the testis and their interactions with morphological differentiation at various stages for male ***,aiming to increase understanding on this topic and facilitate discovery of new techniques for advancing *** aquaculture.

关键词： Macrobrachium rosenbergii Androgenic gland(AG) sex reversal Monosex culture RNAi(dsRNA) Andrectomization

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Expression of sex-Related Genes in Chicken Embryos During Male-to-Female sex reversal Exposure to Diethylstilbestrol

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Journal of Integrative Agriculture 2013年第1期12卷 127-135页

作者： FANG Li-xiu XIN Rui CHE Yi XU Shi-qing Department of Applied Biology Medical College Soochow University

sex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, we constructed a male-to- female sex-reversal model of chick induced by diethylstilbestrol （DES） at onset of incubation （E0）. The results of semi- quantitative PCR showed that the expression of Sf1, the orphan nuclear receptor steroidogenic factor-1 gene, was put forward from E7d to E5d and up-regulated during E5-7d; the Dmrt1, the double sex and the Mab-3 related to transcription factor 1 gene, was down-regulated during E3-7d. Meanwhile, anti-Müllerian hormone gene （Amh） expressed at a similar level in the genetic females and sex-reversal females before E7d, while no expression products of the three female-specific genes Wpkci, Fet1 and Foxl2 were detected in male-to-female embryos. These findings suggest that the expression of some certain sex-related genes, induced by the exogenous estrogen during period of sex determination and gonadal differentiation, results in the male-to-female sex reversal. Moreover, high activity of Sf1 gene during E5-7d might be related to the profemale process, while low activity of Dmrt1 gene during E3-5d might be anti-male. The expression activity of Amh gene might only contribute to the promale process after E7d, however, it is possibly not an anti-female gene in chick embryos.

关键词： sex reversal diethylstilbestrol chicken sex determination sexual differentiation sex-related gene

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sexual plasticity in bony fishes:Analyzing morphological to molecular changes of sex reversal

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Aquaculture and Fisheries 2022年第5期7卷 525-539页

作者： Swathi Tenugu Balasubramanian Senthilkumaran Department of Animal Biology School of Life SciencesUniversity of HyderabadP.O.Central UniversityHyderabad500046India

sex reversal is one of the characteristic properties of sexual plasticity in bony fishes wherein both natural and induced sex change happens at various stages of life cycle in different *** determination in gonochoristic species is genetically regulated,wherein the same sex is retained throughout their life span whereas hermaphrodites change their sex during development or *** sequential hermaphrodites,serial sex change occurs at different points of life ***,synchronous hermaphrodites function as both the sexes during *** variables like temperature,pH and social factors can trigger sex reversal in *** reversal through gene mutations and chemicals/hormones,including sex steroids,can be induced mostly at early developmental stages but natural sex reversal can occur at any *** reversal mechanism shows morphological to molecular changes,which are ideal for identification of sex-specific gene *** fact,gonadal transdifferentiation occurs at the molecular level through differential expression of transcription factors and steroidogenic enzyme genes vis-a-vis hormones,thereby imparting phenotypic or structural *** addition,brain shows sexual dimorphism which is mostly consequential to gonadal sex development and occasionally either *** major breakthrough in this line is the identification of sex determining genes such as dmy/dmrt1Yb,gsdfY,sox3 in the Japanese medaka and amhY in Patagonian ***,the induction of mono-sex population by favouring one sex due to sex-specific differences in growth is an important economic boom for *** review comprehensively highlights key molecular factors involved in natural and induced sex reversal conditions to illustrate teleostean sexual plasticity and its application perspectives.

关键词： sex reversal Sequential hermaphrodite sex steroids Gonadal transdifferentiation sexual dimorphism Teleost

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sex reversal of Black-Chinned Tilapia (Sarotherodon melanotheron) Fry Using 17α-Methyltestosterone

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Journal of Agricultural Science and Technology(A) 2024年第4期14卷 167-180页

作者： Lawrence Armah Ahiah Fisheries Commission Ministry of Fisheries and Aquaculture DevelopmentAccra(GA-184)Ghana

This study was conducted to determine the effective dose of 17α-methyltestosterone(MT)that produces all-male fry of black-chinned tilapia,Sarotherodon *** concentrations:0,30,60,90 and 120 mg 17α-methyltestosterone(17α-MT)per kilogram feed were prepared using the ethanol evaporation method,and administered orally to black-chinned tilapia fry for 28 days in triplicate aquarium glass *** the treatment period,triplicates of 60 fry from each treatment were stocked in hapas and further cultured in freshwater *** were fed with commercial fish feed ad *** sex ratio,growth performance and survival were evaluated after 60 and 180 *** the end of the 60-day period,the sex ratio of 30 fish from each treatment was determined by using the gonadal squash *** remaining 30 fish from each treatment were grown further for 180 days and their sex ratio determined using the genital *** results demonstrated that 17α-methyltestosterone was effective in producing phenotypic male in ***,in both 60-day and 180-day experiments,no treatment gave 100%male *** 90 mg/kg MT yielded the highest all-male fish of 84.13%,highest survival of 70%and highest final mean body weight of 103.99±7.92 g.

关键词： 17α-methyltestosterone sex reversal tilapia growth performance masculinization

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Male sex determination： insights into molecular mechanisms

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Asian Journal of Andrology 2012年第1期14卷 164-171,180页

作者： Kathryn McClelland Josephine Bowles Peter Koopman Institute for Molecular Bioscience The University of Queensland Brisbane QLD 4072 Australia

Disorders of sex development often arise from anomalies in the molecular or cellular networks that guide the differentiation of the embryonic gonad into either a testis or an ovary, two functionally distinct organs. The activation of the Y-linked gene Sry （sex- determining region Y） and its downstream target Sox9 （Sry box-containing gene 9） triggers testis differentiation by stimulating the differentiation of Sertoli cells, which then direct testis morphogenesis. Once engaged, a genetic pathway promotes the testis development while actively suppressing genes involved in ovarian development. This review focuses on the events of testis determination and the struggle to maintain male fate in the face of antagonistic pressure from the underlying female programme.

关键词： gonadal knockout mice sex determination sex-determining region Y protein sex disorders sex reversal sexual development testis

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The role of sex chromosomes in mammalian germ cell differentiation： can the germ cells carrying X and Y chromosomes differentiate into fertile oocytes？

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Asian Journal of Andrology 2015年第3期17卷 360-366页

作者： Teruko Taketo Department of Surgery Research Institute of MUHC McGill University Montreal Quebec Canada Department of Biology McGill University Montreal Quebec Canada Department of Obstetrics and Gynecology McGill University Montreal Quebec Canada

The sexual differentiation of germ cells into spermatozoa or oocytes is strictly regulated by their gonadal environment, testis or ovary, which is determined by the presence or absence of the Y chromosome, respectively. Hence, in normal mammalian development, male germ cells differentiate in the presence of X and Y chromosomes, and female germ cells do so in the presence of two X chromosomes. However, gonadal sex reversal occurs in humans as well as in other mammalian species, and the resultant XX males and XY females can lead healthy lives, except for a complete or partial loss of fertility. Germ cells carrying an abnormal set of sex chromosomes are efficiently eliminated by multilayered surveillance mechanisms in the testis, and also, though more variably, in the ovary. Studying the molecular basis for sex-specific responses to a set of sex chromosomes during gametogenesis will promote our understanding of meiotic processes contributing to the evolution of sex determining mechanisms. This review discusses the fate of germ cells carrying various sex chromosomal compositions in mouse models, the limitation of which may be overcome by recent successes in the differentiation of functional germ cells from embryonic stem cells under experimental conditions.

关键词： embryonic stem cells germ cells oocyte sex chromosomes sex reversal

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sex determination mechanisms and sex control approaches in aquaculture animals

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Science China(Life Sciences) 2022年第6期65卷 1091-1122页

作者： Xi-Yin Li Jie Mei Chu-Tian Ge Xiao-Li Liu Jian-Fang Gui State Key Laboratory of Freshwater Ecology and Biotechnology Hubei Hongshan LaboratoryThe Innovative Academy of Seed DesignInstitute of HydrobiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesWuhan 430072China College of Fisheries Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China College of Biological and Environmental Sciences Zhejiang Wanli UniversityNingbo 315100China Key Laboratory of Tropical&Subtropical Fishery Resource Application&Cultivation of Ministry of Agriculture and Rural Affairs Pearl River Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhou 510380China

Aquaculture is one of the most efficient modes of animal protein production and plays an important role in global food *** animals exhibit extraordinarily diverse sexual phenotypes and underlying mechanisms,providing an ideal system to perform sex determination research,one of the important areas in life ***,sex is also one of the most valuable traits because sexual dimorphism in growth,size,and other economic characteristics commonly exist in aquaculture ***,we synthesize current knowledge of sex determination mechanisms,sex chromosome evolution,reproduction strategies,and sexual dimorphism,and also review several approaches for sex control in aquaculture animals,including artificial gynogenesis,application of sex-specific or sex chromosome-linked markers,artificial sex reversal,as well as gene *** anticipate that better understanding of sex determination mechanisms and innovation of sex control approaches will facilitate sustainable development of aquaculture.

关键词： sex determination sex control approach sexual dimorphism sex chromosome sex change reproduction strategy sex reversal

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Overexpression of Kdm6b induces testicular differentiation in a temperature-dependent sex determination system

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Zoological Research 2024年第5期45卷 1108-1115页

作者： Qiran Chen Wei Sun Lin Jin Yingjie Zhou Fang Li Chutian Ge College of Biological and Environmental Sciences Zhejiang Wanli UniversityNingboZhejiang 315100China Institute of Animal sex and Development Zhejiang Wanli UniversityNingboZhejiang 315100China

In reptiles,such as the red-eared slider turtle(Trachemys scripta elegans),gonadal sex determination is highly dependent on the environmental temperature during embryonic *** complex process,which leads to differentiation into either testes or ovaries,is governed by the finely tuned expression of upstream genes,notably the testis-promoting gene Dmrt1 and the ovary-promoting gene *** studies have identified epigenetic regulation as a crucial factor in testis development,with the H3K27me3 demethylase KDM6B being essential for Dmrt1 expression in ***,whether KDM6B alone can induce testicular differentiation remains *** this study,we found that overexpression of Kdm6b in *** embryos induced the male development pathway,accompanied by a rapid increase in the gonadal expression of Dmrt1 at 31°C,a temperature typically resulting in female ***,this sex reversal could be entirely rescued by Dmrt1 *** findings demonstrate that Kdm6b is sufficient for commitment to the male pathway,underscoring its role as a critical epigenetic regulator in the sex determination of the red-eared slider turtle.

关键词： Temperature-dependent sex determination sex reversal Kdm6b Dmrt1 T.s.elegans

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