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Early embryonic transcriptomes of Zeugodacus tau provide insight into sex determination and differentiation genes

Insect Science 2022年第3期29卷 915-931页

作者： Peipei Liu Wenping Zheng Jiao Qiao Ziniu Li Zhurong Deng Yimin Yuan Hongyu Zhang Key Laboratory of Horticultural Plant Biology(MOE) Hubei Hongshan LaboratoryChina-Australia Joint Research Center for Horticultural and Urban PestsInstitute of Urban and Horticultural EntomologyCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina College of Environmental Design Wuhan Institute of Design and SciencesWuhanChina

Zeugodacus tau(Walker)is an invasive pest.The sterile insect technique is an environment-friendly method for pest control.Understanding the mechanism of sex determination will contribute to improving efficiency of this technique.In this study,we identified the transformer(tra)gene in Z.tau.One female-specific and two male-specific isoforms of tra were found in Z.tau,and the male-specific splicing pattern of tra was found to occur 5 h after egg laying.We performed transcriptome sequencing at 1 h(E1),5 h(E5),and 9 h(E9)after egg laying and obtained high-quality transcriptome libraries of early embryo development.We identified 13297 and 11713 differentially expressed genes(DEGs)from E5 versus E1 and E9 versus E1 comparisons,respectively.To explore the potential functions of the DEGs during embryonic development,Gene Ontology,Clusters of Orthologous Groups of proteins,and Kyoto Encyclopedia of Genes and Genomes analyses were performed.Twenty-six genes potentially involved in sex determination or differentiation,including Maleness-on-the-Y(MoY),were identified in Z.tau.To verify the transcriptome results,15 genes were selected for quantitative real-time PCR validation.The results were consistent with the transcriptome sequencing results.Moreover,U2 small nuclear riboprotein auxiliary factor(U2AF-50),female lethal d(fl(2)d),and virilizer(vir)were highly expressed at E5,indicating that they may be related to the sex-specific splicing of tra.Further functional analysis is needed to confirm this speculation.Our data provide an insight into the mechanism underlying sex determination and differentiation in tephritid species.

关键词： RNA-Seq sex determination sex differentiation transformer Zeugodacus tau

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A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish

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Water Biology and Security 2022年第3期1卷 77-87页

作者： Ding Ye Yi-Xuan Tu Houpeng Wang Mudan He Yaqing Wang Zhengfang Chen Zhen-Xia Chen Yonghua Sun State Key Laboratory of Freshwater Ecology and Biotechnology Hongshan LaboratoryInstitute of HydrobiologyInnovation Academy for Seed DesignChinese Academy of SciencesWuhan430072China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing100049China Hubei Hongshan Laboratory Huazhong Agricultural UniversityWuhan430070China Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityShenzhen518000China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen518000China

Zebrafish(Danio rerio)has been used as a promising animal model to study gonadal development and gametogenesis.Although previous studies have identified critical molecules participating in zebrafish gonad differentiation,a landscape view of the biological processes involved in this process is still lacking.Here we isolated intact zebrafish differentiating gonads,at 25 days post-fertilization(dpf)and 30 dpf and conducted RNA-seq analyses on the juvenile gonads that tended to develop into ovaries or testes.Our study demonstrates that the juvenile ovary and testis at 25 dpf and 30 dpf are different at the biological process level.During ovary differentiation,the biological processes related to metabolic activities in the production of energy and maternal substances,RNA degradation,and DNA repair were enriched.During testis differentiation,the biological processes related to cell proliferation,differentiation,and morphogenesis were enriched,with a total of 15 signaling pathways.Notably,we reveal that the immune-related processes are extensively involved in the regulation of testis development.Overall,this study provides a landscape of differentiated biological processes and novel insights into the initiation of sex differentiation in zebrafish.

关键词： Gonad differentiation Maternal materials Ovary sex differentiation Spermatogenesistestis Zebrafish

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Impact of xenoestrogens on sex differentiation and reproduction in teleosts

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Aquaculture and Fisheries 2022年第5期7卷 562-571页

作者： Brototi Roy Reetuparna Basak Umesh Rai Department of Zoology Maitreyi CollegeUniversity of DelhiDelhi110021India Department of Zoology Bhaskaracharya College of Applied SciencesUniversity of DelhiDelhi110075India Department of Zoology University of DelhiDelhi110007India

Among vertebrates,teleosts display a wide array of sex determination and differentiation mechanisms ranging from chromosomal sex determination on one end of the spectrum to environmental sex determination on the other end.However,the interplay of both these mechanisms is also not uncommon.Several gonochoristic fishes exhibit gonadal plasticity often resulting in sex reversal.The major manipulation of sex differentiation in teleost is affected by sex steroids.In this context,the increasing contamination of aquatic ecosystems by estrogen-like compounds,commonly known as xenoestrogens,is of major concern.This often leads to deleterious effects on the reproductive success of fish and thereby adversely impacts aquatic biodiversity.In the present review,we have focused on impact of xenoestrogen at different levels of the reproductive system influencing not only gonadal differentiation in teleosts but also their reproductive functions.The review would also explore the mitigation strategies and regulations in place for aquatic xenoestrogen management.

关键词： Xenoestrogen Teleost sex differentiation Gonad Intersex Reproduction

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Two sex pheromone receptors for sexual communication in the American cockroach

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Science China(Life Sciences) 2024年第7期67卷 1455-1467页

作者： Na Li Renke Dong Huanchao Zeng Yan Zhang Run Huang Wei Liu Fengming Cao Jincong Yu Mingtao Liao Jingyou Chen Wenlei Zhang Zejian Huang Jiahui Wang Li Li Shen Zhu Danyan Huang Zining Li Xiaoshuai Zhang Dongwei Yuan Nan Chen Yongliang Fan Guirong Wang Coby Schal Yufeng Pan Sheng Li Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou 510631China Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhou 514589China Lingnan Guangdong Laboratory of Modern Agriculture Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of AgricultureNorthwest A&F UniversityYangling 712100China Department of Entomology and Plant Pathology North Carolina State UniversityRaleigh 27695USA The Key Laboratory of Developmental Genes and Human Disease School of Life Science and TechnologySoutheast UniversityNanjing 210096China

Volatile sex pheromones are vital for sexual communication between males and females.Females of the American cockroach,Periplaneta americana,produce and emit two sex pheromone components,periplanone-A(PA)and periplanone-B(PB).Although PB is the major sex attractant and can attract males,how it interacts with PA in regulating sexual behaviors is still unknown.In this study,we found that in male cockroaches,PA counteracted PB attraction.We identified two odorant receptors(ORs),OR53 and OR100,as PB/PA and PA receptors,respectively.OR53 and OR100 were predominantly expressed in the antennae of sexually mature males,and their expression levels were regulated by the sex differentiation pathway and nutrition-responsive signals.Cellular localization of OR53 and OR100 in male antennae further revealed that two types of sensilla coordinate a complex two-pheromone-two-receptor pathway in regulating cockroach sexual behaviors.These findings indicate distinct functions of the two sex pheromone components,identify their receptors and possible regulatory mechanisms underlying the male-specific and age-dependent sexual behaviors,and can guide novel strategies for pest management.

关键词： sex pheromone odorant receptor sexual attraction sex differentiation cockroach

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Self-fertilization in a gonochoristic fish yellow catfish

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Aquaculture and Fisheries 2024年第2期9卷 234-244页

作者： Yue Yu Ziyi Lu Min Chen Ya Liu Yang Ding Chenhao Hu Zhigang Shen College of Fisheries Key Lab of Freshwater Animal BreedingMinistry of Agriculture and Rural AffairsWuhan430070China Key Lab of Agricultural Animal Genetics Breeding and Reproduction of Ministry of EducationWuhan430070China Engineering Technology Research Center for Fish Breeding and Culture in Hubei Province Wuhan430070China Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt of Ministry of Education Huazhong Agricultural UniversityWuhan430070China Hubei Huangyouyuan Fishery Development Limited Company Wuhan430070China

sex control techniques are in great demand for aquaculture because of the huge benefits of mono-sex production.In a previous study on yellow catfish(Tachysurus fulvidraco),intersex individuals were produced by administrating 17α-methyltestosterone(MT)or aromatase inhibitor letrozole(LZ).The ovarian and testicular portions of intersexual gonads developed well and were completely separated,arousing our interest in producing self-fertilizable fish and clone-like fish populations.In this study,yellow catfish fry were treated with a combination of MT and LZ at a low dose(5 mg kg^(-1)MT,300 mg kg^(-1)LZ)at a low water temperature(27.0℃,mixed sex-producing temperature,LL group)and a high temperature(33.5◦C,male-producing temperature,HL group),and with a high dose(10 mg kg^(-1) MT,600 mg kg^(-1) LZ)at a low water temperature(LH group),during 12-65 days post-hatching,the critical period of sex differentiation.Both XX and XY intersex were produced with different percentages at the same time through co-treatment with MT and LZ,with or without the help of male-producing temperature.The highest percentage of XY intersex(53%in XY,36%in XX and XY)and XX intersex(47%in XX,25%in XX and XY)was produced in LH and HL groups,respectively.Results of gonadal morphology,histology,and sperm dynamic analysis indicate that some XX and XY intersex individuals were capable of self-fertilization through artificial insemination.The reproductive performance of these intersex individuals was then investigated by injecting spawning agents,dissecting gonads,and artificially fertilizing eggs with their own sperm.The XX,XY,and YY genotypic individuals were produced by the self-fertilization of XY intersex individuals.The present study successfully produced high percentages of XX and XY intersex and conducted self-fertilization by mating the eggs and sperm of a single intersex individual.Self-fertilization in fish will show great potential in aquaculture genetics and breeding,for example,generating highly homozygous(or"clonal")genetic material,produc

关键词： sex differentiation Self-fertilization sex reversal Mono-sex Clone

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Identification and Characterization of Long Non-Coding RNAs Involved in sex-Related Gene Regulation in Kelp Saccharina japonica

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Journal of Ocean University of China 2023年第3期22卷 755-765页

作者： BAI Meihan YUE Shutao WANG Weiwei DU Xinxin LI Xiaojie YANG Guanpin ZHANG Linan School of Marine Science and Engineering Qingdao Agricultural UniversityQingdao 266109China National Engineering Science Research&Development Center of Algae and Sea Cucumbers of China Provincial Key Laboratory of Genetic Improvement&Efficient Culture of Marine Algae of ShandongShandong Oriental Ocean Sci-Tech Co.Ltd.Yantai 264003China Marine Science Research Institute of Shandong Province Qingdao 266104China College of Marine Life Sciences Ocean University of ChinaQingdao 266003China

Long non-coding RNAs(lncRNAs)regulate a variety of biological processes,including sexual reproduction and differentiation.Saccharina japonica,a commercially important brown alga in China,shows remarkable sexual dimorphism in haploid gametophytes.The sex of Saccharina japonica gametophytes is determined by UV sexual system.However,no results have been reported on the lncRNAs involved in the sex-related gene regulation of S.japonica.This study identified a number of lncRNAs and assessed their expression levels in male and female gametophytes.Among them,a total of 405 lncRNAs and 211 mRNAs showed differential expressions.Furthermore,the functions of target genes of differentially expressed lncRNAs(DELs)differed from those of differentially expressed genes(DEGs),suggesting that lncRNA may interact with other functional proteins,in addition to DEGs,to involve sex regulation in S.japonica.There were 32 and 90 potential cis-regulatory and trans-regulatory interactions between DELsDEGs,respectively.Five of these lncRNAs(LNC_002974,LNC_021059,LNC_038466,LNC_051584,and LNC_027400)interacted with putative male sex determination region(SDR)genes,suggesting that they act as regulators in gametophytes'sex regulation potentially.Findings from this study contribute to our understanding of the roles of lncRNAs in sex differentiation and lay the foundation for functional studies of candidate lncRNAs in the future.

关键词： Saccharina japonica long non-coding RNAs sex differentiation gametophyte brown algae

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Dynamics of sexual development in teleosts with a note on Mugil cephalus

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Aquaculture and Fisheries 2022年第5期7卷 507-518页

作者： J.Logamanya Tilak Angeline Samuel A.Kalarani R.Moses Inbaraj Endocrinology Unit Department of ZoologyMadras Christian CollegeTambaramChennai600059India

The process of sex determination(SD)and sex differentiation(Sd)with associated sex-specific behaviour in teleosts is brought about by genetic factors and environmental factors under the influence of chemical messengers.SD is initiated by inherited genes,which in turn influence Sd by the production of hormones.To understand the plasticity of SD in fishes,the functional role of genes must be clearly elucidated.During early development,the dimorphic expression of male and female are mediated by the differentially sexualized brain.In mammals,SD region(SDR)on the Y chromosome(SRY)can be considered as male-specific copy of SOX3 and in the absence of SRY in teleosts suggests SD might be regulated by alternate genes.DM(doublesex/mab)-related genes,AMH(anti-mullerian hormone),TGF-b(transforming growth factor beta),GSDF(gonadal soma derived growth factor)and other genes(around 18)located in chromosomes of teleosts are found to be responsible for SD.The SD gene,sdY(sexually dimorphic on the Y chromosome)has been detected in more than 15 salmonid species,and in medaka and tilapia SD system is governed by heterogametic mechanism.In mullet,Mugil cephalus Dor et al.identified 27 SDR genes and suggested to be potential candidate genes for SD.Recently whole-genome sequencing data were produced from mullet and assembled into a draft genome sequence in which>30 loci are potentially associated with SD.Further analysis is required to know the involvement of each sex-biased gene for its functional influence on sex.Investigators attempted to know the sex-linkage group in tilapia and found that 2 linkage group i.e.LG1 and LG22 show SD loci,whereas in mullet it was documented that LG8 is the SD loci.Rigorous study with all male population(YY)or knockout of male marker gene,might give conclusive understanding on SD in future.The review highlights the absence of genomic understanding of SD and SDR genes for futuristic direction of research.

关键词： sex determination SRY sdY sex differentiation Mugil cephalus

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Transcriptional diversity of DMRT1(dsx-and mab3.related transcription factor1)in human testis

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Cell Research 2006年第4期16卷 389-393页

作者： Ken McElreavey Department of Genetics and Center for Developmental Biology College of Life Sciences Wuhan University Wuhan 430072 China Department of Developmental Biology Institut Pasteur 25 rue du Dr Roux 75724 Paris Cedex 15 France

Recent advances in the evolutionary genetics of sex determination indicate that the only molecular similarity in sexdetermination found so far among phyla is between the fly doublesex,worm mab-3 and vertebrate DMRTI(dsx-andmab3-related transcription factor 1)/DMY genes.Each of these factors encodes a zinc-finger-like DNA-binding motif,DM domain.Insights into the evolution and functions of human DMRT1 gene could reveal evolutionary mechanismsof sexual development.Here we report the identification and characterization of multiple isoforms of human DMRT1in the testis.These transcripts encode predicted proteins with 373,275 and 175 amino acids and they were generatedby alternative splicing at 3’region.Expression level of DMRT1a is higher than those of both DMRT1b and c,and theDMRTlc expression was the lowest in testis,based on comparisons of mean values from real-time fluorescent quantita-tive RT-PCR analysis.Both DMRTlb and c result from exonization of intronic sequences,including the exonization ofan Alu element.A further search for Aiu elements within the DMRT1 gene demonstrated that all 99 Alu elements arenon-randomly distributed among the non-coding regions on both directions.These new characteristics of DMRT1 wouldhave an important impact on the evolution of sexual development mechanisms.

关键词： evolution alternative splicing doublesex sex differentiation

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Proteome landscape and spatial map of mouse primordial germ cells

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Science China(Life Sciences) 2021年第6期64卷 966-981页

作者： Pan Wang Yilong Miao Xiao-Han Li Na Zhang Qilong Wang Wei Yue Shao-Chen Sun Bo Xiong Jie Qiao Mo Li Center for Reproductive Medicine Peking University Third HospitalBeijing 100191China College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China National Clinical Research Center for Obstetrics and Gynecology Beijing 100191China Key Laboratory of Assisted Reproduction(Peking University) Ministry of EducationBeijing 100191China

Primordial germ cells(PGCs)are precursors of both male and female gametes as fundamental materials for organism development.The transcriptome,methylome,and chromatin accessibility profiles of PGCs in both mice and humans have been recently reported.However,little is known about the characteristics of PGCs at the protein levels,which directly exert cellular functions.Here,we construct landscapes of both proteome and 3D spatial distribution of mouse PGCs at E11.5,E13.5 and E16.5 days,the three critical developmental windows for PGCs'sex differentiation,female meiosis initiation and male mitotic arrest.In each developmental stage of PGCs,nearly 2,000–3,000 proteins are identified,among which specific functional pathways such as oxidative phosphorylation,DNA damage repair,and meiotic cell cycle are involved for different events during PGCs development.Interestingly,by 3D modeling we find that PGCs spatially cluster into around 1,300 nests in genital ridge at E11.5 and the nest number is not increased by the exponential proliferation of PGCs.Comparative analysis of our proteomic data with published transcriptomic data does not show a close correlation,meaning that the practically executive factors are beyond the transcriptome.Thus,our work offers a valuable resource for the systematic investigations of PGC development at protein level and spatial map.

关键词： PGCs proteome spatial map sex differentiation meiosis

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A CCCH zinc finger gene regulates doublesex alternative splicing and male development in Bombyx mori

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Insect Science 2021年第5期28卷 1253-1261页

作者： Fangying Yang Zhongjie Zhang Bo Hu Ye Yu Anjiang Tan Key Laboratory of Insect Developmental and Evolutionary Biology CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai200032China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeiing100049China

Recent identification of a Piwi-interacting RNA(piRNA)-initiated sex determination cascade in the silkworm,Bombyx mori,provides novel insights into high diversity of insect sex determination pathways.In this system,the W-chromosome-derived Fem piRNA is the primary sex determination signal.A CCCH-type zinc finger gene Masculinizer(Masc),which is targeted by Fem piRNA-PIWI complex in female animals,is indispensable for male-specific splicing of B.mori doublesex(Bmdsx).Although many genes involved in this cascade have been identified,the regulatory mechanisms of silk-worm sex determination remain to be elucidated.Here we show that another CCCH-type zinc finger gene,Bmznf-2,is a masculinization factor in B.mori.Bmznf-2 shows testisabundant expression and loss of Bmznf-2 function via clustered regularly interspaced short palindromic repeats 1 single-guide RNA-mediated mutagenesis results in feminized differentiation and appearance of the female-specific splicing variants of Bmdsx transcripts in males.In contrast,there is no phenotypic consequence in mutant females.In mutant males,relative messenger RNA expression levels of female-dominant genes such as vitellogenin and sex-specific storage protein I are significantly clevated while several male-dominant genes are significantly down-regulated.Furthermore,male mutants show delayed developmental timing,smaller body sizes of larvae and malformation of moth wings.Our data thus reveal that Bmznf-2 plays an indispensable role in silkworm male sexual differentiation.

关键词： Bmznf-2 Bombyx mori CRISPR/Cas9 sex differentiation

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