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First Italian outbreak of VIM-producing serratia marcescens in an adult polyvalent intensive care unit, August-October 2018: A case report and literature review

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World Journal of Clinical Cases 2019年第21期7卷 3535-3548页

作者： Maria Rosaria Iovene Vincenzo Pota Massimiliano Galdiero Giusy Corvino Federica Maria Di Lella Debora Stelitano Maria Beatrice Passavanti Maria Caterina Pace Aniello Alfieri Sveva Di Franco Caterina Aurilio Pasquale Sansone Vettakkara Kandy Muhammed Niyas Marco Fiore Department of Experimental Medicine University of Campania"Luigi Vanvitelli"Naples 80138Italy Department of Women Child and General and Specialized SurgeryUniversity of Campania"Luigi Vanvitelli"Naples 80138Italy Department of Medicine All India Institute of Medical SciencesNew Delhi 110029India

BACKGROUND Carbapenem-resistant Enterobacteriaceae has become a significant public health concern as hospital outbreaks are now being frequently reported and these organisms are becoming difficult to treat with the available *** SUMMARY An outbreak of VIM-producing serratia marcescens occurred over a period of 11 wk(August,1 to October,18)in patients admitted to the adult polyvalent intensive care unit of the University of Campania“Luigi Vanvitelli”located in *** episodes occurred in three patients(two patients infected,and one patient colonized).All the strains revealed the production of *** After three decades of carbapenem antibiotics use,the emergence of carbapenemresistance in Enterobacteriaceae has become a significant concern and a stricter control to preserve its clinical application is *** is,to our knowledge,the first outbreak of VIM-producing serratia marcescens in *** policies must be implemented to avoid future outbreaks.

关键词： serratia marcescens Carbapenamase VIM Intensive care unit Outbreak Case report

沙雷氏菌serratia ***-98发酵生产2-酮基-D-葡萄糖酸的工艺优化及动力学研究

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化工学报 2011年第5期62卷 1371-1376页

作者：张炜谢志鹏罗玮张建国浙江大学生物化学研究所浙江杭州310058 浙江大学生物工程研究所浙江杭州310027

引言 2-酮基-D-葡萄糖酸（2-KDG）有着广泛的用途,它能被用作食品添加剂、水泥增塑剂、洗涤剂,是照片显影剂的重要成分;同时它也是除草剂、D-核酮糖、D-阿拉伯糖,特别是D-异抗坏血酸合成过程中的重要前体。

关键词： 2-酮基-D-葡萄糖酸发酵工艺优化动力学模型 serratia sp.BK-98

Continuous Fermentation of a Prodigiosin-Producing serratia marcescens Strain Isolated from Soil

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Advances in Bioscience and Biotechnology 2019年第4期10卷 98-108页

作者： Qi Fu Yujuan Xiao Xuehui Duan Huabin Huang Zhixia Zhuang Jinhai Shen Yangyue Pei Haojia Xu Meiyu Gan Department of Science and Technology for Inspection Xiamen Huaxia University Xiamen China State Key Laboratory of Food Science and Technology Nanchang China

A red-pigmented bacterial strain NS-17 was isolated from soil, and physiological and biochemical characteristics and 16S rDNA analysis established this organism as a strain of serratia marcescens. The red pigment isolated from cells of NS-17 was identified as prodigiosin. By analyzing factors affecting the production of prodigiosin, modified medium and culture conditions were set up, and a continuous fermentation method was carried out to take advantage of the mass production of foam during fermentation. Continuous fermentation was achieved in the following conditions: 32℃, 1:1 ventilation ratio, and medium with a nutrient concentration 5-fold higher than that of the fermentation medium (with half the inorganic salt concentration) supplied as a feed medium at a flow rate of 8 mL/min. For the first time of continuous fermentation of serratia marcescens coupling with foam flotation, a high yield of prodigiosin was obtained. After 56 h of culturing, the total harvest of cells was enhanced 2.33-fold compared to that of batch fermentation, the total amount of prodigiosin was enhanced 2.70-fold compared to that of batch fermentation, and cells and prodigiosin were concentrated in the efflux broth automatically.

关键词： serratia marcescens Prodigiosin Foam Continuous Fermentation Automatic Concentration

Phosphate Solubilization by Bacillus subtilisand serratia marcescensIsolated from Tomato Plant Rhizosphere

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Journal of Environmental Protection 2018年第3期9卷 266-277页

作者： Eman A. H. Mohamed Azza G. Farag Sahar A. Youssef Botany and Microbiology Department Faculty of Science Damanhour University Damanhour Egypt Department of Biotechnology Faculty of Science Taif University Taif KSA Department of Virus and Phytoplasma Research Institute of Plant Pathology Research Agriculture Research Centre Cairo Egypt

Plants need phosphorus for many physiological activities in a form of phosphate anions. Three different bacterial strains (Bacillus subtilis PH, serratia marcescens PH1, and serratia marcescens PH2), recently isolated from tomato plant rhizosphere, have high phosphate solubilization index (SI from 2.8 to 3.2) on Pikovskaya agar medium (which contains calcium phosphate). Moreover, phosphate release from calcium in Pikovskaya broth over 5 days is increasing with cell growth for the different isolates. The most efficient phosphate solubilization case is the mixed culture of the 3 strains (OD475 is almost 1). On the other hand, pH values decreased dramatically with time due to organic acids secretion and the maximum acidification level is recoded for serratia marcescens PH2 (pH = 1.94). Interestingly, the isolates are resistance to important pesticides (oxamyl, thiophanate methyl, and captan) that are commonly used in the sampling area. This resistance is very favorable and increases the persistence of the phosphate solubilizing bacteria in contaminated soils. The isolates are therefore plant symbionts and growth promoting agents.

关键词： Phosphate Solubilization Pikovskaya Medium Bacillus subtilis serratia marcescens

serratia marcescens非特异性核酸内切酶的原核表达及其应用

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中国生物制品学杂志 2009年第7期22卷 667-670页

作者：张开俊杨莉成都恩多施生物工程技术有限公司成都610041 四川大学生物治疗国家重点实验室成都610041

目的在大肠杆菌中表达serratia marcescens非特异性核酸内切酶(SMNE),并进行纯化、活性检测及应用。方法合成smne基因,应用PCR技术在基因的5′端引入6个组氨酸标签序列,将其插入分泌表达载体pET-20b(+)中,转化大肠杆菌BL21(DE3)pLysS,I... 详细信息

目的在大肠杆菌中表达serratia marcescens非特异性核酸内切酶(SMNE),并进行纯化、活性检测及应用。方法合成smne基因,应用PCR技术在基因的5′端引入6个组氨酸标签序列,将其插入分泌表达载体pET-20b(+)中,转化大肠杆菌BL21(DE3)pLysS,IPTG诱导表达。表达产物经镍离子螯合琼脂糖凝胶一步纯化后,检测其活性并计算比活。将纯化的SMNE用于重组腺病毒的制备,对外源性核酸进行降解,并采用Southern blot对外源性DNA残留量进行测定。结果重组表达质粒pET-20b-smne经PCR、双酶切和测序证明构建正确。重组蛋白的表达量为8.0 mg/L,纯化后纯度达95%,比活达1.1×106 U/mg。在重组腺病毒制备过程中使用后,成品中的外源性DNA残留量≤10 ng/5.0×1011 VP。结论已成功地在大肠杆菌中表达了SMNE,纯化的SMNE活性高,有望应用于重组生物制品制备过程中外源性核酸的去除。

关键词： serratia marcescens 非特异性核酸内切酶原核表达外源性DNA

serratia marcescensRhabdomyolysis

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Advances in Infectious Diseases 2013年第2期3卷 63-64页

作者： Miguel F. Carrascosa José R. Salcines-Caviedes María Carmen Farinas Internal Medicine Department Hospital of Laredo Laredo Spain

serratia marcescens has been recognized as an important cause of nosocomial and community-acquired infections. To our knowledge, we describe the first case of S. marcescens rhabdomyolysis, most probably related to acute cholecystitis and secondary bacteremia. The condition was successfully managed with levofloxacin. Keeping in mind the relevant morbidity and mortality associated with bacterial rhabdomyolysis, physicians should consider this possibility in patients with suspected or proven bacterial disease. We suggest S. marcescens should be regarded as a new causative agent of infectious rhabdomyolysis.

关键词： Acute Cholecystitis Acute Renal Failure Myositis Rhabdomyolysis serratia marcescens

昆虫病原线虫共生菌serratia nematodiphila DR186对果蝇致病机制的研究

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南京农业大学学报 2017年第5期40卷 827-834页

作者：徐漫蔡翰林张克云南京农业大学生命科学学院江苏南京210095

[目的]通过昆虫病原线虫共生菌serratia nematodiphila DR186侵染黑腹果蝇的野生型和免疫突变体,研究该共生菌侵染果蝇成虫的致病机制。[方法]利用饲喂和针刺方式将*** DR186接入野生型黑腹果蝇和其体液免疫途径突变体的肠道和血腔中引... 详细信息

[目的]通过昆虫病原线虫共生菌serratia nematodiphila DR186侵染黑腹果蝇的野生型和免疫突变体,研究该共生菌侵染果蝇成虫的致病机制。[方法]利用饲喂和针刺方式将*** DR186接入野生型黑腹果蝇和其体液免疫途径突变体的肠道和血腔中引起侵染;通过统计2种侵染条件下各个突变体的存活率,血腔中共生菌的菌体数,结合荧光定量PCR检测两类抗菌肽Diptericin和Drosomycin的表达水平来研究*** DR186对果蝇的致病机制。[结果]*** DR186对野生型黑腹果蝇的肠道侵染存活率在7 d降至0,血腔侵染存活率在19 h降至0;*** DR186血腔侵染后,Imd途径的PGRP-LC、Dredd、Relish突变体相对Toll途径的Dif突变体和野生型更加敏感,在12 h内存活率低于50%,并在随后短时间内全部死亡。荧光定量PCR分析显示Diptericin和Drosomycin的相对表达水平在血腔侵染过程中均呈先上升再逐渐降低的趋势。Dredd突变体Drosomycin相对表达量在6 h达到88%,Dif突变体和野生型的Diptericin相对表达量在6 h分别达到75%和62%。*** DR186侵染肠道后对Imd途径和Toll途径突变体及野生型的存活率在7 d降至0。针刺法侵染的致病性高于饲喂法侵染,在19 h存活率降至0。[结论]*** DR186可能采用免疫逃逸机制从果蝇肠道成功侵染果蝇。果蝇抵抗*** DR186侵染的主要免疫途径是Imd体液免疫途径。

关键词：昆虫病原线虫共生菌黑腹果蝇体液免疫 serratia nematodiphila DR186

冰川细菌serratia sp.A_(29)-2发酵产低温蛋白酶条件的优化

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食品与发酵工业 2014年第4期40卷 71-76页

作者：武运张晓燕周建中尹明远王小标苗森韩艳鹏马蕊新疆农业大学食品科学与药学学院新疆乌鲁木齐830052

为提高新疆冰川细菌serratia sp.A29-2的低温蛋白酶产酶活性,在单因素试验的基础上,通过正交试验和响应面试验,对其发酵培养基和发酵条件进行了优化。研究表明:其最佳培养基组成为蛋白胨15.0 g/L、酵母粉5.0 g/L、麦芽糖10.0 g/L、CaCl2... 详细信息

为提高新疆冰川细菌serratia sp.A29-2的低温蛋白酶产酶活性,在单因素试验的基础上,通过正交试验和响应面试验,对其发酵培养基和发酵条件进行了优化。研究表明:其最佳培养基组成为蛋白胨15.0 g/L、酵母粉5.0 g/L、麦芽糖10.0 g/L、CaCl20.5 g/L。最优发酵条件是:温度22℃,pH值7.0,时间60 h,NaCl质量分数1.0%。以优化培养基为基础,在最适发酵条件下,菌株serratia sp.A29-2产低温蛋白酶酶活可达54.25 U/mL,是初始酶活的1.93倍。

关键词：低温蛋白酶 serratia sp.A29-2 产酶优化

Isolation　and　purification　of　CWF－Ⅰ　derived　from　serratia　marcescens　and　its　antitumor　activities　in　vitro

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Journal of Medical Colleges of PLA(China) 1994年第3期9卷 194-198页

作者：焦炳华赵虎余伟明周丙荣许伟群王梁华

The antitumor effect of serratia marcescens was recognized as early as in the 1930s,and the mechanism involved was proposed to be mediated by endotoxin moieties present in the outer membrane of its cell wall.A new active component which shares potential antitumor activities in vitro was isolated and purified to homogeneity by the extraction and purification protocol established in our *** its tumor cytocidal effect was studied using light,scanning and transmission electron microscopies.

关键词： serratia marcesces cell wall fraction preparation morphological examinations