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Mechanism of dorsal root ganglion SERT in electroacupuncture regulation of P2X3 receptor-mediated visceral hypersensitivity in IBS rats

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Journal of Acupuncture and Tuina Science 2024年第6期22卷 435-446页

作者： HUANG Rong CHAI Jing ZHOU Yun QIAO Yu WENG Zhijun WU Huangan LIU Huirong ZHU Lu MA Jindan ZHU Yi ZHANG Fang Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese MedicineShanghai 200437China Shanghai Research Institute of Acupuncture and Meridian Shanghai 200030China

Objective:To investigate the role of serotonin reuptake transporter(SERT)and P2X3 receptor of dorsal root ganglion(DRG)in regulating visceral hypersensitivity of rats with irritable bowel syndrome(IBS)by electroacupuncture(EA).Methods:Male Sprague-Dawley and SERT^(-/-)rats were subjected to preparing IBS visceral hypersensitivity models with 2,4,6-trinitrobenzene sulfonic acid(TNBS)*** weeks post-modeling,interventions including EA,intrathecal injection,and EA plus intrathecal injection were applied,***-eosin staining and abdominal withdrawal reflex(AWR)score were used to confirm the successful establishment of the IBS *** score,whole-cell patch clamp technique,and Western blotting assay were used to evaluate the changes in visceral pain sensitivity,electrophysiological properties of DRG neurons,and the expression of DRG P2X3 receptor and SERT in IBS ***:Compared to the model group,the AWR score in the EA group decreased significantly(P<0.05),the resting membrane potential(P<0.05)and the number of action potentials(P<0.05)of DRG neurons reduced,and the baseline intensity increased(P<0.05);additionally,the expression of P2X3 receptor in DRG decreased(P<0.01),and the SERT expression increased(P<0.05).Compared to the P2X3 receptor agonist group,the SERT protein expression in DRG was higher in the EA *** SERT^(-/-)rats,the P2X3 receptor expression in DRG increased in the EA group compared to the model group(P<0.01).Conclusion:EA modulates the electrophysiological characteristics of intestinal primary sensory neurons by regulating the expression of SERT and P2X3 receptor in DRG of IBS *** modulation may contribute to the mechanism by which EA alleviates peripheral sensitization of visceral pain in IBS rats.

关键词： Acupuncture Therapy Electroacupuncture Irritable Bowel Syndrome Visceral Hypersensitivity Visceral Pain Receptors,Purinergic P2X3 serotonin reuptake transporter Rats

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