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Exploring the attenuation mechanisms of Dalbergia odorifera leaves extract on cerebral ischemia-reperfusion based on weighted gene co-expression network analysis

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BIOCELL 2023年第7期47卷 1611-1622页

作者： JINFANG HU JIANGEN AO LONGSHENG FU YAOQI WU FENG SHAO TIANTIAN XU MINGJIN JIANG SHAOFENG XIONG YANNI LV Department of Pharmacy The First Affiliated Hospital of Nanchang UniversityNanchang330006China Department of Pharmacy The Second Affiliated Hospital of Nanchang UniversityNanchang330006China Key Laboratory of Modern Preparation of TCM Ministry of EducationJiangxi University of Traditional Chinese MedicineNanchang330006China Jiangxi Provincial Institute of Translational Medicine The First Affiliated Hospital of Nanchang UniversityNanchang330006China

The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion(I/R)is little *** candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity *** molecular mechanism of *** leaves on cerebral I/R was ***:serial affinity chromatography based on *** leaves extract(DLE)affinity matrices were applied to find specific binding proteins in the brain tissues implemented on C57BL/6 mice by intraluminal middle cerebral artery occlusion for 1 h and reperfusion for 24 *** binding proteins were subjected to mass-spectrometry to search for the differentially expressed proteins between control and DLE-affinity *** hub genes were screened based on weighted gene co-expression network analysis(WGCNA).Then,predictive biology and potential experimental verification were performed for the candidate *** protective role of DLE in blood-brain barrier damage in cerebral I/R mice was evaluated by the leakage of Evans blue,western blotting,immunohistochemistry,and immunofluorescent ***:952 differentially expressed proteins were classified into seven modules based on WGCNA under soft threshold *** on WGCNA,AKT1,PIK3CA,NOS3,SMAD3,SMAD1,IL6,MAPK1,TGFBR2,TGFBR1,MAPK3,IGF1R,LRG1,mTOR,ROCK1,TGFB1,IL1B,SMAD2,and SMAD518 candidate hub proteins were involved in turquoise ***-β,MAPK,focal adhesion,and adherens junction signaling pathway were associated with candidate hub *** ontology analysis demonstrated that candidate hub proteins were related to the TGF-βreceptor signaling pathway,common-partner SMAD protein phosphorylation,*** could significantly reduce the leakage of Evans blue in mice with cerebral I/R,while attenuating the expression of occludin,claudin-5,and zonula *** blotting demonstrated that regulation of TGF-β/SMAD signaling pathway played an essential role in the protective effect of ***:

关键词： Dalbergia odorifera leaves serial affinity chromatography WGCNA Cerebral ischemia-reperfusion TGF-β SMADS

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