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Separation and purification of GST-glycerol-3-phosphate Dehydrogenase

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Agricultural Biotechnology 2016年第5期5卷 44-45页

作者： Hongmei ZHAO Shihai LI Yasuo WATANABE College of Animal Sciences Yangtze University Agriculture Department Ehime University

In order to investigate the expression of glycerol-3 -phosphate dehydrogenase by GCY1 gene in recombinant Saccharomyces cerevisiae, induction culture of the S. cerevisiaestrain was performed with SD-URA 2% galactose, 3 × YP ＋ 6% glucose, SC-URA 2% galactose, and SC-URA 2% galactose ＋ 5% NaCI glyeerol-3-phosphate dehydregenase, the cultured S. cerevisiaewas comminuted followed by full-automatic high-speed purification, and SDS-PAGE gel electrophoresis was performed for molecular weight of the GST fusion protein. The results showed that after shaking culture of the S. cerevisiae containing GCY1 at 25 ℃, the OD values of its 3 × YP ＋ 6% glucose culture and SC-URA 2% galaetose ＋ 5% NaC1 culture were 8.75 and 7.35, respectively. It was shown by purification with a Profinia low-pressure liquid chromatograph that only the S. cerevisiae cultured in SC-URA 2% galactose ＋ 5% NaC1 medium expressed glycerel-3-phosphate de- hydrogenase, the molecular weight of which was detected as 65 ku by SDS-PAGE gel electrophoresis.

关键词： Saccharomyces cerevisiae Glycerol-3-phosphate dehydrogenase Galactose SDS-PAGE gel electrophoresis Separation and purification

Recovery of platinum from spent automotive catalyst based on hydrometallurgy

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Rare Metals 2023年第4期42卷 1118-1137页

作者： Tao Ge Jin-Dong He Liang Xu Yan-Hang Xiong Ling Wang Xiao-Wei Zhou Yong-Pan Tian Zhuo Zhao School of Metallurgical Engineering Anhui University of TechnologyMa’anshan243032China State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization Kunming650093China Low-Carbon Research Institute Anhui University of TechnologyMa’anshan243032China

Platinum(Pt)is a critical raw material for automotive catalytic converters due to its high-temperature stability,corrosion resistance and catalytic activity,whereas its limited primary resources and uneven distribution make it hard to meet the growing demand of platinum.Spent automotive catalyst(SAC)is currently the most important secondary resource of platinum,of which the platinum content is much higher than that of the primary platinum resources.The recovery process of platinum from spent automobile catalyst mainly consists of pretreatment followed by enrichment and refining,involving pyro-and hydrometallurgical techniques,among which enrichment and refining processes are extremely important for platinum recovery from spent automobile catalyst.This paper provides an overview of the technologies for platinum recovery from spent automotive catalyst.The emphasis is placed on the processes of enrichment and refining based on hydrometallurgical techniques.Future directions of research and development of platinum recovery from spent automobile catalyst are also proposed.

关键词： Platinum(Pt) Platinum group metals Spent automotive catalyst(SAC) Hydrometallurgy Solvent extraction Separation and purification

A new microfiltration membrane with three-dimensional reticular architecture for Nano-pollutants removal from wastewater

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Progress in Natural Science:Materials International 2021年第3期31卷 414-419页

作者： Wanyuan Gui Jingru Liu Xiping Song Hui Zhang Junpin Lin Benli Luan National Center for Materials Service Safety State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing Department of Chemistry Western University Qian Xuesen Laboratory of Space Technology China Academy of Space Technology

This research provides a new insight into designing next-generation filtration materials for Nano-pollutants removal from wastewater, and potentially for gas/solid or liquid/solid separation in corrosive environment.Considering the high corrosion and oxidation resistance, Ti–40 Al–10 Nb–10 Cr porous alloy was prepared by powder metallurgy and used as a mechanical support with a pore size of around 10 μm. Subsequently, metallic Zn nanoparticles was coated on the Ti–40 Al–10 Nb–10 Cr porous framework by means of chemical vapor deposition,obtaining a novel microfiltration membrane with three-dimensional reticular architecture. Furthermore, the influence of Zn coating on surface morphology, pore feature, and filtration capability of Ti–40 Al–10 Nb–10 Cr porous alloy was investigated. It is of note that the as-prepared microfiltration membrane exhibits an excellent Nanopollutants filtration efficiency of greater than 99.989% from wastewater. Most importantly, such a threedimensional reticular architecture provides excellent structural stability, allowing the reutilization of the microfiltration membrane for at least 200 filtration cycles of wastewater treatment.

关键词： Porous materials Microfiltration membrane Separation and purification Intermetallic materials Water purification

Technology development for rare earth cleaner hydrometallurgy in China

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Rare Metals 2015年第4期34卷 215-222页

作者： Xiao-Wei Huang Zhi-Qi Long Liang-Shi Wang Zong-Yu Feng National Engineering Research Center for Rare Earth Materials General Research Institute for Nonferrous Metals Grirem Advanced Materials Co. Ltd.

China is the global leader in rare earth （RE） production using hydrometallurgical processes. Advantageous extraction techniques of rare earths from Baotou mixed rare earth minerals, bastnaesite, and ion-adsorption clays of rare earth deposits have been developed in China. The Separation and purification technologies have also achieved rapid development. The industrial application processes for rare earth hydrometallurgy were summarized in the present paper. With the large demands and rapid development of rare earths, the issues of resources and environment are more prominent. This review gives an overview of the main processes that were developed in the past toward greener hydrometallurgy of rare earths in China. Based on the development of the rare earth industry, comprehensive utilization and cleaner production should still be focused on in the future, to support the sustainable development.

关键词： Rare earths Cleaner production Separation and purification Hydrometallurgy

purification and characterization of Al_(13) species in coagulant polyaluminum chloride

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Journal of Environmental Sciences 2009年第1期21卷 18-22页

作者： GAO Baoyu CHU Yongbao YUE Qinyan WANG Yan School of Environmental Science and Engineering Shondong University Jinan 250100 China. School of Environment and Safety Engineering Qingdong University of Science and Technology Qingdao 266042 China Key Laboratory of Colloid and Interface Chemistry Minstry of Education Shandong University Jinan 250100 China

Nano-Al13 was separated and purified by four methods to investigate its characteristic, and was analyzed by Al-Ferron timed complexation spectrophotometer, ^27Al-NMR （nuclear magnetic resonance）, and transmission electron microscopy （TEM）. Coagulation efficiency of nano-Al13, polyaluminum chloride （PAC）, and AlCl3 in synthetic water were also investigated by jar test. The dynamic process and aggregation state of kaolin suspensions coagulating with nano-Al13, PAC, and AlCl3 were also investigated. The experimental results indicated that the efficiency of gel column chromatography method was the highest for separating PAC solution with low Al concentration. Ethanol and acetone method was simple and could separated PAC solution with different Al concentrations, while silicon alkylation white block column chromatography method could separate PAC solution only with low Al concentration. The SO4^2-/Ba^2＋ displacement method could separate PAC solution with high Al concentration, but extra inorganic cation and anion could be introduced into the solution during the separation. The coagulation efficiency and dynamic experimental results showed that nano- Al13 with a high positive-charged species was the main species of electric neutralization in coagulation process, and it could reduce the turbidity and increase the effective particles collision rate efficiently in coagulation process. Its coagulation speed and the particle size of coagulant formed were of greatest value in this study.

关键词： nano-Al13 Separation and purification coagulation dynamic processes

Isolation and characterization of lignans from the leaves of Isatis indigotica Fortune

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Asian Journal of Traditional Medicines 2020年第3期15卷 103-112页

作者： Shuang Qiu Peng Zhao Yufei Xi Xiaoxiao Huang Shaojiang Song Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research&Development Liaoning ProvinceSchool of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyang 110016China

A phytochemical investigation of 80%EtOH exact of Isatis indigotica Fortune leaves yielded ten lignans(1-10),including six furofurans lignans(1-6),one arylnaphthalenes lignans(7)and three tetrahydrofurans lignans(8-10).Their structures were determined by HRESIMS spectroscopic data and extensive NMR analyses.Among them,compounds 6,9 and 10 were isolated from the genus oi Isatis for the first time.

关键词： Isatis indigotica Separation and purification lignans structural identification

Phenolic compounds from Elephantopus scaber L.

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Asian Journal of Traditional Medicines 2020年第3期15卷 121-126页

作者： Yijie Zhang Ming Bai Weiyu Zhou Xiaoxiao Huang Shaojiang Song Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research&Development Liaoning ProvinceSchool of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyang 110016China

Eight compounds,(+)-syringaresinol(1),apigenin(2),3,5-dimethoxy-4-hydxyphenylpropanol(3),3,4,5-trimethoxy benzoic acid,(4)ferulaic acid(5),trans-p-hydroxycinnamic methyl ester(6),4-hydroxy-3-methoxybenz-oic acid(7)and p-methoxy benzoic acid(8)were isolated from the 70%ethanol extract of the whole herb of Elephantopus scaber L.The structures of all compounds were identified by spectral data analysis.All compounds were obtained for the first time from Elephantopus scaber L..

关键词： Elephantopus scaber L. Separation and purification structural analysis phenolic compounds

Isolation and identification of chemical constituents from Elephantopus scaber Linn.

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Asian Journal of Traditional Medicines 2021年第2期16卷 98-104页

作者： Wei Xu Ming Bai Xiaoxiao Huang Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research&Development Liaoning ProvinceSchool of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyang 110016China

Elephantopus scaber L.,as a popular Chinese medicinal herb of the Compositae family,is widely distributed in America,Africa and Asia.In the present study,five compounds were isolated from the 70%EtOH extract of the whole herb from Elephantopus scaber L.,including two lignans and three flavones.Their chemical structures were identified based on NMR spectroscopic analysis and comparison with the data reported in literature.It is noteworthy that compounds 1-5 were isolated from Elephantopus scaber L.for the first time and their chemotaxonomic significance was discussed.

关键词： Elephantopus scaber L. Separation and purification chemical constituents structural elucidation

Phenolic compounds from Peanut testa

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Asian Journal of Traditional Medicines 2020年第4期15卷 186-192页

作者： Zhiyong Xu Yufei Xi Xiaoxiao Huang Shaojiang Song Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research&Development Liaoning ProvinceSchool of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyang 110016China

Phytochemical investigation of peanut testa(the seed coat of Arachis hypogaea L.)led to the isolation of eight phenolic compounds,including caffeic acid(1),methyl caffeate(2),ethyl caffeate(3),methyl protocatechuate(4),ethyl protocatechuate(5),butyl protocatechuate(6),(E)-p-hydroxycinnamic acid methyl ester(7),and resveratrol(8).The structures of the compounds were elucidated through spectroscopic data analysis and comparison with the previously reported literature.Among them,compounds 2,3,5,and 6 were obtained from Arachis hypogaea L.for the first time.

关键词： peanut testa A rachis hypogaea L. Separation and purification phenolic compoimds