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Effect of cholesterol-loaded cyclodextrin enriched extenders on the quality of pre- frozen and frozen buffalo semen

Asian pacific Journal of Reproduction 2022年第3期11卷 146-152页

作者： Asmaa A.Mostafa Mohamed S.El-Belely Sayed T.Ismail Reda I.El-Sheshtawy Mohamed I.Shahba Abassia Frozen semen Center General Organization for Veterinary ServicesCairoEgypt Department of Theriogenology Faculty of Veterinary MedicineCairo UniversityGizaEgypt Department of Animal Reproduction and Artificial Insemination National Research CentreDokkiGizaEgypt

Objective:To investigate the effects of non-permeable cryoprotectant,cholesterol-loaded cyclodextrin,when added at different concentrations into cooled and frozen-thawed semen extended with Tris-citrate-fructose egg yolk glycerol and lecithin-based extenders.Methods:A total of 40 ejaculates from four buffalo bulls were collected using artificial vagina.Ejaculates were extended with one of Tris-citrate-fructose egg yolk glycerol and lecithin-based extenders which contained different concentrations[0(control),0.75,1.50,2.25 and 3.00 mg/mL]of cholesterol-loaded cyclodextrin.The extended semen samples were cooled to 5曟and then frozen slowly to-196曟in 0.25 mL ministraws before being stored in liquid nitrogen pending its evaluation.Sperm motility,live sperm,normal sperm morphology,sperm membrane integrity and acrosome morphology were measured.Results:Supplementation of cholesterol-loaded cyclodextrin improved progressive motility,viability,morphology and acrosome as well as plasma membrane integrities at 1.50-2.25 mg/mL depending upon types of used extenders and stages of pre-and post-freezing process(P<0.01).The best concentration was 1.50 mg/mL at pre-freeze stage and 2.25 mg/mL at post-freezing.However,greater concentration(3.00 mg/mL)of cholesterol-loaded cyclodextrin had a detrimental effect compared to the control group with the two evaluated extenders(P<0.01).Conclusions:Cholesterol-loaded cyclodextrin supplementation at 1.50-2.25 mg/mL concentration could improve pre-frozen and post-thawed buffalo sperm quality.The most suitable concentration is 1.50 mg/mL at pre-freeze stage and 2.25 mg/mL at post-freezing.

关键词： Buffalo semen Tris Cooling Cryopreservation Cholesterol-loaded cyclodextrin semen evaluation

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Current updates on laboratory techniques for the diagnosis of male reproductive failure

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Asian Journal of Andrology 2016年第3期18卷 392-401,I0008页

作者： Suresh C Sikka Wayne JG Hellstrom Department of Urology Tulane University School of Medicine New Orleans Louisiana USA.

The incidence of male reproductive failure leading to infertility, whether due to delayed parenthood, environmental issues, genetic factors, drugs, etc., is increasing throughout the world. The diagnosis and prognosis of male subfertility have become a challenge. While the basic semen assessment has been performed for many years, a number of studies question the value of the traditional semen characteristics. This is partly due to inadequate methods and standardization, limited knowledge of technical requirements for quality assurance, and an incomplete understanding of what clinical information a semen assessment can provide. Laboratories currently performing semen and endocrine assessment show great variability. The World Health Organization （WHO） manual for the evaluation of semen has been the core of andrology and fertility evaluation that has helped in further development of this field over many years. These include the physical appearance of the ejaculate, assessments of sperm count, motility, vitality, morphology, and functional aspects of the sperm and semen sample. These tests also include male endocrine profile, biochemical evaluation of the semen, detection of antisperm antibodies in serum, the use of computer-aided sperm analysis （CASA）, sperm DNA integrity, and its damage due to oxidative stress. Assisted reproductive techniques （e.g., IVF, ICSI） have shown great success but are too expensive. Further development in this field with newer techniques and extensive training/instructions can improve accuracy and reduce variability, thus maintaining the quality and standards of such an evaluation. There is an urgent need to have standardized training centers and increased awareness in this area of men＇s health for reproductive success.

关键词： male infertility quality control management reproductive failure semen evaluation World Health Organization manual

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