检索结果-南通市图书馆

Journal of Pharmaceutical Analysis 2015年第6期5卷 378-382页

作者： Vinayagam Rambabu Subramaniyan Suba Suburamaniyan Vijayakumar P.G. and Research Department of Botany and Microbiology A.V.V.M. Sri Pushpam College (Autonomous) K.K Biotec Lab Service

Cancer is a communal health hazard worldwide. The present investigation attempts to evaluate anti- microbial and anticancer potential of kosinostatin on mammary carcinoma cell line （MCF-7）. The anticancer and antiproliferative activities of kosinostatin were analyzed on MCF cell line by MTT assay and cytotoxicity assays like lactate dehydrogenase （LDH） and glutathione （GSH）. The secondary metabolite kosinostatin exhibited its apoptotic nature by expressing p53 protein. Collectively, the results acquired from this study promise that kosinostatin shows the potent anticancer activity.

关键词： Kosinostatin secondary metabolite Streptomyces sp. Cytotoxic activity MCF-7

Anticancer Activity of Rice Callus Suspension Cultures from Aromatic Varieties and metabolites Regulated in Treated Cancer Cell Lines

在线全文

学校读者我要写书评

暂无评论

Rice science 2024年第4期31卷 449-462,I0027-I0030页

作者： Anuradha KUMARI Wusirika RAMAKRISHNA Department of Biochemistry Central University of PunjabPunjab 151401India

Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.

关键词： anticancer cytotoxicity metabolite profiling plant tissue culture rice callus suspension culture secondary metabolite

Paenarthrobacter nitroguajacolicus can promote cucumber growth and control cucumber corynespora leaf spot

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2024年第3期11卷 485-498页

作者： Min LI Yahui HUANG Xiaomin DONG Xu ZHANG Qing MA College of Horticulture South China Agricultural University State Key Laboratory of Crop Stress Biology for Arid Areas/College of Plant Protection Northwest A&F University College of Forestry and Landscape Architecture South China Agricultural University

Currently, the disease control in cucumber mainly depends on agrochemicals,which is not an environmentally benign strategy. Biocontrol bacteria not only resist plant pathogens but also promote plant growth, which is ecofriendly and sustainable option. A biocontrol bacterial strain BJ-5 was screened using Corynespora cassiicola as the target pathogen, and BJ-5 was determined to be Paenarthrobacter nitroguajacolicus by morphological and molecular *** effect of BJ-5 on C. cassiicola was studied, including the spore germination, cell membrane permeability and infected cucumbers. BJ-5 inhibited the germination of C. cassiicola spores in vitro and led to atrophy and deformation of the C. cassiicola budding tubes. BJ-5 caused the relative extracellular conductivity of C. cassiicola mycelia to increase compared with the control. Additionally, BJ-5 reduced the severity of cucumber corynespora leaf spot of cucumber infected with C. cassiicola. The inhibition efficacy of BJ-5 suspension as a foliar spray against cucumber corynespora leaf spot reached 63% inhibition, which is higher than a 5000-fold dilution of Luna-Son SC fungicide. In addition, BJ-5 was tested on the emergence of cucumber seedlings, recording the biomass and photosynthesis of cucumber during the growth period. BJ-5 at 1.5 × 10～5 CFU·m L-1promoted the germination of cucumber seeds and increased biomass and photosynthesis at the adult plant stage. Also, the secondary metabolites of BJ-5 were determined. BJ-5 could produce chitinases, siderophore, cellulase, amylase and protease in the respective medium. Finally, adaptation assay of BJ-5 showed good salt tolerance and good adaptability in alkaline conditions, and that BJ-5 retains inhibition of fungi activity at higher temperatures. This is the first report of the biocontrol by P. nitroguajacolicus with antagonism to C. cassiicola and promote cucumber growth. This study indicates that P. nitroguajacolicus may serve as potential biocontrol agents against

关键词： Adaptability antagonistic bacterium disease resistance growth promotion secondary metabolite

A Comprehensive Review on Preclinical Safety and Toxicity of Medicinal Plants

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Clinical Complementary Medicine and Pharmacology 2024年第1期4卷 95-111页

作者： Madhav Nilakanth Mugale Kapil Dev Bhumika S.More Vaishali Sunil Mishra Kaveri R.Washimkar Kishan Singh Rakesh Maurya Srikanta Kumar Rath Debprasad Chattopadhyay Naibedya Chattopadhyay Division of Toxicology and Experimental Medicine CSIR-Central Drug Research Institute(CSIR-CDRI)Lucknow 226031India Division of Phytochemistry CSIR-Central Institute of Medicinal and Aromatic Plants(CSIR-CIMAP)Lucknow 226015India Medicinal and Process Chemistry Division CSIR-CDRI Lucknow 226031India Division of Endocrinology CSIR-CDRI Lucknow 226031India Division of Ethnomedicine ICMR-National Institute of Traditional MedicineNehru NagarBelagavi 590010and ICMR-Virus UnitKolkata 700010India Department of Health Research NSHM Knowledge CampusKolkata 700051India

Background:Globally,80%people use plant-derived products for treating or preventing *** prevalent perception about medicinal plants is that they are safe and devoid of adverse ***,approximately 1,50,000 plants contain toxic ***:The present review focuses on medicinal plant extracts/fractions toxicity assessments made in preclin-ical models by oral ***:Detail studies were searched from databases including PubMed and Google Scholar.A manual reference screening of the selected studies was done to identify relevant articles,with no language restriction being imposed at the time of ***:The studies included were performed in rodents,and the test substances were administered *** search revealed 33 widely used plants or products with significant toxicity,and phytochemicals from these plants have been *** a systematic review,we identified a plethora of medicinal plant extracts reporting safety and toxicity ***:In the future,preclinical toxicokinetic studies of herbs and the determination of their no-observed-adverse-effect levels are required for a complete safety ***,the interaction of herbs with com-monly used/over-the-counter drugs in terms of the latter’s metabolic profile should be undertaken.

关键词： Extract Herbal medicine Toxicity Rodent secondary metabolite

Engineering of a genome-reduced host: practical application of synthetic biology in the overproduction of desired secondary metabolites

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2010年第7期1卷 621-626页

作者： Hong Gao Ying Zhuo Elizabeth Ashforth Lixin Zhang CAS Key Laboratory of Pathogenic Microbiology&Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)Beijing 100101China Graduate University of Chinese Academy of Sciences Beijing 100049China

Synthetic biology aims to design and build new biological systems with desirable properties,providing the foundation for the biosynthesis of secondary *** most prominent representation of synthetic biology has been used in microbial engineering by recombinant DNA ***,there are advantages of using a deleted host,and therefore an increasing number of biotechnology studies follow similar strategies to dissect cellular networks and construct genomereduced *** review will give an overview of the strategies used for constructing and engineering reduced-genome factories by synthetic biology to improve production of secondary metabolites.

关键词： synthetic biology reduced-genome secondary metabolite

In silico methods for linking genes and secondary metabolites: The way forward

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2016年第2期1卷 80-88页

作者： Shradha Khater Swadha Anand Debasisa Mohanty Bioinformatics Center National Institute of ImmunologyAruna Asaf Ali MargNew Delhi 110067India

In silico methods for linking genomic space to chemical space have played a crucial role in genomics driven discovery of new natural products as well as biosynthesis of altered natural products by engineering of biosynthetic *** we give an overview of available computational tools and then briefly describe a novel computational framework,namely retro-biosynthetic enumeration of biosynthetic reactions,which can add to the repertoire of computational tools available for connecting natural products to their biosynthetic gene *** of the currently available bioinformatics tools for analysis of secondary metabolite biosynthetic gene clusters utilize the“Genes to metabolites”*** contrast to the“Genes to metabolites”approach,the“metabolites to Genes”or retro-biosynthetic approach would involve enumerating the various biochemical transformations or enzymatic reactions which would generate the given chemical moiety starting from a set of precursor molecules and identifying enzymatic domains which can potentially catalyze the enumerated biochemical *** this article,we first give a brief overview of the presently available in silico tools and approaches for analysis of secondary metabolite biosynthetic *** also discuss our preliminary work on development of algorithms for retro-biosynthetic enumeration of biochemical transformations to formulate a novel computational method for identifying genes associated with biosynthesis of a given polyketide or nonribosomal peptide.

关键词： secondary metabolite Polyketides Nonribosomal peptides Genes to metabolites metabolites to genes Genome mining Retro-biosynthetic enumeration Biosynthetic gene cluster

Epigenetic modification associated with climate regulates betulin biosynthesis in birch

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Forestry Research 2023年第1期34卷 21-35页

作者： Jiang Wang Bowei Chen Shahid Ali Tianxu Zhang Yu Wang He Zhang Lishan Wang Yonglan Zhang Linan Xie Tingbo Jiang Jing Yin Heike W.Sederoff Gaurav Zinta Ronald R.Sederoff Yuhua Li Qingzhu Zhang State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040People’s Republic of China College of Life Sciences Northeast Forestry UniversityHarbin 150040People’s Republic of China Forest Biotechnology Group Department of Forestry and Environmental ResourcesNorth Carolina State UniversityRaleighNC 27695USA Biotechnology Division CSIR-Institute of Himalayan Bioresource TechnologyPalampur 176061India

The Betula genus contains pentacyclic triterpenoid betulin known for its environmental adaptation and medicinal ***,the mechanisms underlying betulin biosynthesis responding to climate change remain *** this study,the role of epigenetic modification(DNA methylation) in betulin biosynthesis was examined and how climatic factors influence ***-genome bisulfite sequencing was performed for greenhouse-grown Chinese white birch(Betula platyphylla Sukaczev) treated with DNA methylation inhibitor zebularine(ZEB) and a natural birch population in Northeast *** treatment significantly affected the CHH methylation level of transposable elements and betulin content in a hormesis dose-dependent *** methylation and expression of bHLH9,a key transcriptional factor controlling betulin biosynthesis,were also consistently affected by ZEB treatment as a hormetic *** the natural population,there was a positive correlation between promoter methylation of bHLH9 and summer precipitation,while winter temperature was negatively *** climate-dependent methylation of bHLH9 regulates the expression of downstream genes involved in betulin *** study highlights the role of environmental signals to induce epigenetic changes that result in betulin production,possibly helping to develop resilient plants to combat ongoing climate change and enhance secondary metabolite production.

关键词： Epigenetics DNA methylation Betulin bHLH9 transcription factor Hormesis Climate change secondary metabolite

RNA Methylome Reveals the m^(6)A-mediated Regulation of Flavor metabolites in Tea Leaves under Solar-withering

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2023年第4期21卷 769-787页

作者： Chen Zhu Shuting Zhang Chengzhe Zhou Caiyun Tian Biying Shi Kai Xu Linjie Huang Yun Sun Yuling Lin Zhongxiong Lai Yuqiong Guo College of Horticulture Fujian Agriculture and Forestry UniversityFuzhou 350002China Institute of Horticultural Biotechnology Fujian Agriculture and Forestry UniversityFuzhou 350002China Tea Industry Research Institute Fujian Agriculture and Forestry UniversityFuzhou 350002China

The epitranscriptomic mark N6-methyladenosine(m^(6)A),which is the predominant internal modification in RNA,is important for plant responses to diverse *** environmental stresses caused by the tea-withering process can greatly influence the accumulation of specialized metabolites and the formation of tea ***,the effects of the m^(6)A-mediated regulatory mechanism on flavor-related metabolic pathways in tea leaves remain relatively *** performed an integrated RNA methylome and transcriptome analysis to explore the m^(6)Amediated regulatory mechanism and its effects on flavonoid and terpenoid metabolism in tea(Camellia sinensis)leaves under solar-withering *** changes in global m^(6)A level in tea leaves were mainly controlled by two m^(6)A erasers(CsALKBH4A and CsALKBH4B)during solar-withering *** methylated peak-associated genes following solarwithering treatments with different shading rates were assigned to terpenoid biosynthesis and spliceosome *** analyses indicated that CsALKBH4-driven RNA demethylation can directly affect the accumulation of volatile terpenoids by mediating the stability and abundance of terpenoid biosynthesis-related transcripts and also indirectly influence the flavonoid,catechin,and theaflavin contents by triggering alternative splicing-mediated *** findings revealed a novel layer of epitranscriptomic gene regulation in tea flavor-related metabolic pathways and established a link between the m^(6)A-mediated regulatory mechanism and the formation of tea flavor under solar-withering conditions.

关键词： Camellia sinensis RNA methylation Epitranscriptome secondary metabolite Withering

Transcriptional regulation of secondary metabolism and autophagy genes in response to DNA replication stress in Setosphaeria turcica

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2023年第4期22卷 1068-1081页

作者： MENG Ya-nan ZHANG Xin-jie ZENG Fan-li ZHAI Wei-bo LI Pan HU Jing-jing XIAO Sheng-lin HAO Zhi-min CAO Zhi-yan CHEN Chuan DONG Jin-gao State Key Laboratory of North China Crop Improvement and Regulation Hebei Agricultural UniversityBaoding 071001P.R.China College of Life Sciences Hebei Agricultural UniversityBaoding 071001P.R.China Key Laboratory of Microbial Diversity Research and Application of Hebei Province School of Life SciencesHebei UniversityBaoding 071001P.R.China Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaoding 071001P.R.China College of Plant Protection Hebei Agricultural UniversityBaoding 071001P.R.China

The fungal pathogen Setosphaeria turcica causes northern corn leaf blight(NCLB),which leads to considerable crop *** turcica elaborates a specialized infection structures called appressorium for maize ***,we demonstrated that the *** triggers an S-phase checkpoint and ATR(Ataxia Telangiectasia and Rad3 related)-dependent self-protective response to DNA genotoxic insults during maize ***,how the regulatory mechanism works was still largely ***,we report a genome wide transcriptional profile analysis during appressorium formation in the present of DNA replication *** performed RNA-Seq analysis to identify *** genes responsive to DNA replication *** the current work,we found that appressorium-mediated maize infection by *** is significantly blocked by S-phase checkpoint.A large serial of secondary metabolite and melanin biosynthesis genes were blocked in appressorium formation of *** during the replication *** secondary metabolite biosynthesis genes including alcohol dehydrogenase GroES-like domain,multicopper oxidase,ABCtransporter families,cytochrome P450 and FAD-containing monooxygenase were related to plant pathogen *** addition,we demonstrated that autophagy in *** is up-regulated by ATR as a defense response to *** identified StATG3,StATG4,StATG5,StATG7 and StATG16 genes for autophagy were induced by ATR-mediated S-phase *** therefore propose that in response to genotoxic stress,*** utilizes ATR-dependent pathway to turn off transcription of genes governing appressorium-mediated infection,and meanwhile inducing transcription of autophagy genes likely as a mechanism of self-protection,aside from the more conservative responses in eukaryotes.

关键词： Setosphaeria turcica appressorium secondary metabolite autophagy DNA replication stress

Improve Anthocyanin and Zinc Concentration in Purple Rice by Nitrogen and Zinc Fertilizer Application

在线全文

学校读者我要写书评

暂无评论

Rice science 2022年第5期29卷 435-450页

作者： Suchila UTASEE Sansanee JAMJOD Sittisavet LORDKAEW Chanakan PROM-U-THAI Department of Plant and Soil Sciences Faculty of Agriculture/Center of Agricultural Resource SystemsFaculty of Agriculture/Lanna Rice Research CenterChiang Mai UniversityChiang Mai 50200Thailand

Zinc(Zn)is an essential micronutrient for plant growth and development,and anthocyanin is a secondary metabolite compound generally produced under stress conditions;both have benefits to human *** is a staple food crop for most of the world’s population,and purple rice is well known as a natural source of Zn and anthocyanins,but their stability depends upon many *** review focuses on the opportunity to increase Zn and anthocyanin compounds in purple rice grains via Zn and nitrogen(N)management during *** in grain Zn concentration and anthocyanin compounds is found among purple rice varieties,thus presenting a challenge for breeding programs aiming at high grain Zn and anthocyanin *** engineering has successfully achieved a high-efficiency vector system comprising two regulatory genes and six structural anthocyanin-related genes driven by endosperm-specific promoters to engineer purple endosperm rice that can provide new high-anthocyanin *** Zn and anthocyanin concentrations in rice can also be affected by environmental factors during cultivation,e.g.,light,temperature,soil salinity and nutrient(fertilizer)*** N and Zn fertilizer is found to influence the physiological mechanisms of Zn absorption,uptake,transport and remobilization to promote grain Zn accumulation in rice,while N application can improve anthocyanin synthesis by promoting its biosynthesis pathway via the use of phenylalanine as a *** summary,there is an opportunity to improve both grain Zn and anthocyanin in purple rice by appropriate management of Zn and N fertilizers during cultivation for specific varieties.

关键词： purple rice anthocyanin secondary metabolite zinc deficiency fertilizer