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Antioxidant, Anti-Inflammatory Efficacy and HPLC Analysis of <i>Annona muricata</i>Leaves extracts from Republic of Benin

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American Journal of Plant Sciences 2020年第6期11卷 803-818页

作者： Kohonou Arnaud Chabi Nicodème Dah-Nouvlessounon Durand Nounagnon Martial Sognigbe Basile Sina Haziz N’Tcha Christine Kohonou A. Christian Lehmane Halfane Dougnon Victorien Pacôme Noumavo Baba-Moussa Lamine Laboratory of Research in Applied Biology Polytechnic School of Abomey-Calavi University of Abomey-Calavi Cotonou Benin Laboratory of Biology and Molecular Typing in Microbiology Department of Biochemistry and Cell Biology Faculty of Science and Technology University of Abomey-Calavi Cotonou Bénin Research Unit in Applied Microbiology and Pharmacology of Natural Substances Laboratory of Research in Applied Biology Polytechnic School of Abomey-Calavi University of Abomey-Calavi Cotonou Benin

Annona muricata L. (Soursop or Graviola) is a naturally occurring plant seen in Southern part of Africa, traditionally used in Benin to treat various diseases. The present study aimed to investigate phytochemical composition and antioxidant, anti-inflammatory activities of A. muricata leaves extracts. The secondary metabolites of ethanolic and hemi-ethanolic extracts were analysed by HPLC method. The DPPH and FRAP methods were used to evaluate the antioxidant activity. Inhibition of albumin denaturation method was used to evaluate anti-inflammatory activity of the tested extracts of which larval cytotoxicity was studied. The major identified compounds were gallic acid, chlorogenic acid, cafeic acid, tannic acid, ferrulic acid, Rutin. Ascorbic acid exhibited the highest inhibition percentage (83.33% ± 0.50%) of DPPH radical with the lowest IC50 (45.1 ± 0.28 μg/ml). The inhibition of the ferric ion Fe3+ varied (p = 0.0013) according to the extracts type. IC50 values of ferric ion inhibition range from 119.5 ± 3.10 to 250.8 ± 2.13 μg/ml respectively for A. muricata

关键词： A. muricata secondary metabolites extracts Screening Biological Activities Benin

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