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Synergistic effect of schizandrin a and DNaseⅠknockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration

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中医杂志:英文版 2023年第4期43卷 661-666页

作者： ZHU Bin YU Ning WANG Lei TIAN Yue WU Mingfen ZHAO Zhigang Department of Pharmacy Beijing Tiantan HospitalCapital Medical UniversityBeijing 100070China Accreditation Center of Traditional Chinese Medicine Physician of National Administration of Traditional Chinese Medicine Beijing 100120China Department of Endocrinology the Third Affiliated Hospital of Beijing University of Chinese MedicineBeijing 100050China

OBJECTIVE:To explore the synergistic effect of deoxyribonucleaseⅠ(DNaseⅠ)knockdown combined with schizandrin a(Sch A)in protecting islet beta-cells(β-cells)from apoptosis under high-glucose(HG)***:The concentration of Sch A was detected by Cell Counting Kit-8(CCK-8).High glucose-cultured rat insulinoma beta cell line(RIN-M5F)cells were treated with Sch A and transfected with DNaseⅠsmall interfering RNA(siRNA).Cell apoptosis rate and apoptosis-related protein level were examined by flow cytometry and Western blot method *** addition,Na^(+)-K^(+)-adenosine triphosphatease(Na^(+)-K^(+)-ATPase)and Ca^(2+)-Mg^(2+)-ATPase activity,cell membrane potential,and intracellular Ca^(2+)concentration was also examined ***:Our study revealed that HG stimulation can cause a significant increase in DNaseⅠlevel and cell apoptosis ***,Sch A combined with DNaseⅠknockdown can significantly decrease the cell apoptosis rate and apoptosis-related protein levels such as BAX(P<0.05)and Caspase-3(P<0.01).In addition,we also found that the combination of Sch A and DNaseⅠknockdown can dramatically increase cell membrane potential level,Na+-K+-ATPase,and Ca^(2+)-Mg^(2+)-ATPase ***,intracellular Ca^(2+)concentration was also found to be significantly decreased by the synergistic effect of Sch A and DNaseⅠ***:Overall,our study reveals a synergistic effect of Sch A and DNaseⅠknockdown in protectingβ-cells from HG-induced apoptosis.

关键词： high glucose diabetes β-cell apoptosis synergistic effect schizandrin a

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