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High-throughput transcriptional profiling of perturbations by Panax ginseng saponins and Panax notoginseng saponins using TCM-seq

引用

Journal of Pharmaceutical Analysis 2023年第4期13卷 376-387页

作者： Junyun Cheng Jie Chen Jie Liao Tianhao Wang Xin Shao Jinbo Long Penghui Yang Anyao Li Zheng Wang Xiaoyan Lu Xiaohui Fan Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou310058China Future Health Laboratory Innovation Center of Yangtze River DeltaZhejiang UniversityJiaxingZhejiang314100China State Key Laboratory of Medical Molecular Biology Department of Biochemistry and Molecular BiologyInstitute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100005China Innovation Center in Zhejiang University State Key Laboratory of Component-Based Chinese MedicineHangzhou310058China Jinhua Institute of Zhejiang University JinhuaZhejiang321016China

Panax ginseng(PG)and Panax notoginseng(PN)are highly valuable Chinese medicines(CM).Although both CMs have similar active constituents,their clinical applications are clearly *** the past decade,RNA sequencing(RNA-seq)analysis has been employed to investigate the molecular mechanisms of extracts or ***,owing to the limited number of samples in standard RNA-seq,few studies have systematically compared the effects of PG and PN spanning multiple conditions at the transcriptomic ***,we developed an approach that simultaneously profiles transcriptome changes for multiplexed samples using RNA-seq(TCM-seq),a high-throughput,low-cost workflow to molecularly evaluate CM perturbations.A species-mixing experiment was conducted to illustrate the accuracy of sample multiplexing in *** from repeated samples were used to verify the robustness of *** then focused on the primary active components,Panax notoginseng saponins(PNS)and Panax ginseng saponins(PGS)extracted from PN and PG,*** also characterized the transcriptome changes of 10 cell lines,treated with four different doses of PNS and PGS,using TCM-seq to compare the differences in their perturbing effects on genes,functional pathways,gene modules,and molecular *** results of transcriptional data analysis showed that the transcriptional patterns of various cell lines were significantly *** exhibited a stronger regulatory effect on genes involved in cardiovascular disease,whereas PNS resulted in a greater coagulation effect on vascular endothelial *** study proposes a paradigm to comprehensively explore the differences in mechanisms of action between CMs based on transcriptome readouts.

关键词： Panax ginseng Panax notoginseng sample multiplexing RNA sequencing

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