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Protective effects of effective ingredients of Danshen (Radix salviae Miltiorrhizae)and Honghua (Flos Carthami)compatibility after rat hippocampal neurons induced by hypoxia injury

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Journal of Traditional Chinese Medicine 2018年第5期38卷 685-697页

作者： Yu Li Wan Haofang Jin Weifeng Yang Jiehong Li Chang Dai Liuling Ge Lijun Zhou Huifen Wan Haitong He Yu College of Life Science Zhejiang Chinese Medical UniversityHangzhou 310053China College of Pharmaceutical Science Zhejiang Chinese Medical UniversityHangzhou 310053China College of Basic Medical Sciences Zhejiang Chinese Medical UniversityHangzhou 310053China College of Life Science Zhejiang Chinese Medical UniversityHangzhou 3100.53China

OBJECTIVE: To investigate the effective ingredients of Danshen(Radix salviae Miltiorrhizae) and Honghua(Flos Carthami)(Danhong) on protective properties towards neonatal rat hippocampal neurons under hypoxia ***: Primary culture of neonatal rat hippocampal neurons was used to model hypoxia damage on the hippocampus. Methyl thiazolyl tetrazolium(MTT) assay and preliminary experiments were conducted to identify the four effective ingredients of Danhong, along with the injection of positive drug onto hippocampal neurons at a non-toxic dosage level. The cultured cells were randomly divided into 12 groups: the normal group, model group,positive drug control group and nine compatibility groups of the four effective ingredients. Different test methods were applied to determine lactate dehydrogenasein(LDH), total superoxide dismutase(T-SOD), malondialdehyde(MDA), 6-keto prostaglandin F_(la)(6-keto-PGF_(1 a)), thromboxane B_2(TXB_2),mitochondrial membrane potential(MMP), free calcium ions concentration([Ca^(2 +)]i) and early onsetcell ***: Different compatibility groups could inhibit the content of LDH and intracellular calcium overload, increase activity in T-SOD, decrease level of MDA and TXB2, improve level of 6-keto-PGF1 aand MMP, and prevent the early onset cell ***: The compatibility of four effective ingredients of Danhong had protective effect toward hippocampi hypoxia. The mechanism might be related to inhibit oxidative stress damage and cell apoptosis, resist thrombosis, and reduce the intracellular calcium ion of overload.

关键词： salviae Miltiorrhiza,Carthamus tinctorius Hippocampus Neurons Cell hypoxia Antioxidants Thrombosis Apoptosis

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THE EFFECT OF RADIX salviae MILTIORRHIZAE ON THE INHIBITORY ACTIVITY OF VASCULAR SMOOTH MUSCLE CELLS TO THE PLASMINOGEN ACTIVATORS SECRETED BY ENDOTHELIAL CELLS

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Medical Bulletin of Shanghai Jiaotong University 1993年第1期16卷 37-42页

作者：毛申兰张彩英黄桂秋王振义 Shanghai Institute of Hematology Department of PathophysiologySSMU

Cultured porcine endothelial cells (EC) produce and secrete plasminogenactivators (PA). If the serum free media incubated by vascular smooth muscle cells(SMC-CM) were mixed with the same media incubated by endothelial cells (EC-CM),the PA activities of the latter decreased significantly. Cocultivation of EC with SMC alsoresulted in a significant decrease (70.7%) of PA activities produced by EC. Sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of SMC-CMfollowed by reverse fibrin autography demonstrated that the PA inhibitor had a molecularweight of 49000-62000. In this study we also investigated the effect of a Chinese herbalmedicine-Radix salviae Miltiorrhizae (RSM) on the inhibitory activity of SMC. The re-sults showed that RSM significantly decreased the inhibitory activity of SMC against thePA secreted by EC.

关键词： smooth muscle cells endothelial cells plasminogen activator plasminogen activator inhibitor Radix salviae Miltiorrhizae

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EFFECT OF RADIX salviae MILTIORRHIZAE ON GROWTH OF ISOLATED CELLS FROM EMBRYONIC CHICKEN FRONTAL BONE CULTURED IN VITRO (A HISTOCHEMICAL STUDY) Ⅱ.THE DEVELOPMENT AND MATURATION OF OSTEOBLAST-LIKE CELLS

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Medical Bulletin of Shanghai Jiaotong University 1993年第2期16卷 47-53页

作者：徐荣辉柴本甫朱雅萍 Shanghai Institute of Traumatology and Orthopedics SSMU

The isolated osteoblast-like cells from embryonic chicken frontal bone werecultured in vitro and histochemical methods adopted to observe the effect of RadixSalviac Miltiorrhizae (RSM) on proliferation, differentiation, and osteogenic capacity ofthese cells. It was found that: 1. The mitosis and proliferation of the osteoblast-like cellscould be accelerated by RSM, resulting in increased density of the cells in RSM groupas compared with the control. 2. After 48 h, the pseudopodia stretched out and drew backactively in osteoblast-like cells in RSM group. Small particles produced in the cells weresecreted through exocytosis to the extracellular medium. However, in the control group,the capacity to form and secrete these particles was limited. These particles showed posi-tive Alcian blue staining in Alcian blue-Sirius red reaction, so they were acidmucopolysaccharide particles. 3. The osteoblast-like cells could secrete vesicular particles 3micra in diameter. These vesicular particles could be stained with Alcian blue in earlystage, then they could be stained with Sirius red, and finally by Alizarin red S. Thesevesicular particles could aggregate and fuse around the cell colonies, forming bonenodules and bone flakes. The quantity and volume of the bone nodules and flakes inRSM group were larger than in the control group. 4. The bone nodules and flakes couldbe labeled vitally with tetracycline, and show strong yellow fluorescence under thefluorescence microscope. Therefore, these substances were the newly formed bone sub-stances.

关键词： Radix salviae Miltiorrhizae histochemistry osteoblast-like cells cultured in vitro osteogenic capacity

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EFFECT OF RADIX salviae MILTIORRHIZAE ON THE PROCOAGULANT AND FIBRINOLYTIC ACTIVITIES INDUCED BY ENDOTOXIN-TREATED BOVINE ENDOTHELIAL CELLS

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Medical Bulletin of Shanghai Jiaotong University 1992年第2期15卷 75-82页

作者：沈传陆张彩英王振义 Department of Pathophysiology Shanghai Institute of Hematology SSMU Shanghai

Effect of endotoxin, and endotoxin plus Radix salviae Miltiorrhizae （RSM） on bovine aortic endothelial cells （BAECs） for the production of tissue factor （TF）, plasminogen activator （PA） and plasminogen activator inhibitor （PAI） activities as well as prostacyclin （PGI2） content were studied. Stimulation of BAECs with endotoxin （1 μg/ ml） increased the expression of cell surface TF activity, the secretions of PAI activity and PGI2 content. However, PA activity in endotoxin-treated groups didn’t change significantly in comparison with that of the control. RSM could stimulate the secretion of PA activity and prevent the increase in TF and PAI activities from endotoxin-treated BAECs. However, there was no significant difference in PGI2 production between endotoxin-and endotoxin+RSM-treated BAECs. These results indicated that increased procoagulant function and decreased fibrinolytic activity of endothelial cells （ECs） might be one of the mechanisms of DIC in gram-negative septicemia and RSM would be a very important thug in the prevention and the treatment of DIC in gram-negative sepsis.

关键词： Radix salviae Miltiorrhizae endotoxin endothelial cell tissue factor disseminated intravascular coagulation

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Clinical study of adjuvant therapy on ischemic stroke with salviae miltiorrhizae and ligustrazine

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Journal of Acute Disease 2017年第1期6卷 36-40页

作者： Jing-Qin Tian Wei Zhang Seventh Department of Encephalopathy Weifang Hospital of Traditional Chinese MedicineWeifang 261041ShandongChina Second Department of Encephalopathy Weifang Hospital of Traditional Chinese MedicineWeifang 261041ShandongChina

Objective: To study the effect of adjuvant therapy with salviae miltiorrhizae and ligustrazine injection on neural function injury, degree of oxidative stress reaction, chemotactic factor and adhesion molecule in patients with ischemic stroke. Methods: Patients with ischemic stroke admitted in our hospital from August 2013 to October 2015 were retrospectively analyzed and divided into conventional treatment (CT) group receiving conventional treatment and traditional Chinese medicine auxiliary (TCMA) group receiving adjuvant therapy with salviae miltiorrhizae and ligustrazine injection. After the treatment of the first month and the third month, serum was collected to detect the contents of nerve injury molecules, oxidative stress parameters, chemotactic factor and adhesion molecule. Results: After the 4-week treatment, the contents of serum neuron specific enolase, S100 calcium-binding protein B, heart-type fatty acid binding protein, malondialdehyde, advanced oxidation protein products, 8-hydroxy-2'-deoxyguanosine, monocyte chemoattractant protein-1,CD40,CD40 ligand, vascular endothelial cadherin, soluble intercellular adhesion molecule 1 and soluble vascular adhesion molecule 1 in patients ofTCMA group were all significantly lower than those of CT group, and the contents of superoxidase dismutase, glutathione peroxidase and catalase were significantly higher than those of CT group. After the 8-week treatment, the contents of serum neuron specific enolase, S100 calcium-binding protein B, heart-type fatty acid binding protein, malondialdehyde, advanced oxidation protein products, 8-hydroxy-2'-deoxyguanosine, monocyte chemoattractant protein-1,CD40, CD40 ligand, vascular endothelial cadherin, soluble intercellular adhesion molecule 1 and soluble vascular adhesion molecule 1 in patients ofTCMA group were all significantly lower than those of CT group and the contents of superoxidase dismutase, glutathione peroxidase and catalase were significantly higher tha

关键词： Ischemic stroke salviae miltiorrhizae and ligustrazine Neural function Oxidative stress reaction Chemotactic factor Adhesion molecule

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CLINICAL ANALYSIS ON TREATMENT OF 40 CASES OF HYPERLIPEMIA WITH POINT-INJECTION OF RADIX salviae MILTIORRZHIZAE INJECTION

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World Journal of Acupuncture-Moxibustion 1998年第4期8卷 20-22页

作者：王华

In the present paper, the effect of point-injection of Radix salviae Miltiorrhizae injectio on blood-lipid was observed in 65 cases of hyperlipemia. The 65 patients were divided into point-injection group (40 cases who were treated with point-injection of Radix salviae Miltiorrhizae injectio and oral administration of Shujiangzhi) and control group (25 cases who were treated with oral administration of Shujiangzhi only). Results showed that the total effective rate of the point-injection group was significantly higher than that of the control group (Psalviae Miltiorrhizae injectio could effectively raise the level of HDL-C, decrease lower LDL-C and regulate estrogen level of women. The slow releasing action of red sage root solution in the topical acupoint region prolonged and enhanced its effect in lowering blood-lipid. There was a significant difference between the two groups in the effect of lowering blood-fat (P< 0. 01).

关键词： Hyperlipemia Point-injection therapy Hydro-acupuncture Radix salviae Miltiorrhizae injectio

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Simultaneous Determination of Four Phenolic Acids in Radix salviae Miltiorrhizae Formula Granules by QAMS

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Medicinal Plant 2019年第6期10卷 27-29,38页

作者： Hongyan WEI Qiongxi YU Jinguang LU Zhihong JIANG Jingzheng SONG Jian ZHOU Purapharm(Nanning)Pharmaceutical Co. Ltd.Nanning 530007China Macao University of Science and Technology Macao 519020China

[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenolic acids,salvianolic acid B,tanshinol,lithospermic acid and rosmarinic acid in Radix salviae Miltiorrhizae by HPLC and conduct methodological investigation.[Methods]Salvianolic acid B,with moderate retention time,good resolution,great response value and easy availability was selected as the internal standard,and the relative retention values and relative correction factors between tanshinol,rosmarinic acid,lithospermic acid and salvianolic acid B were *** results of QAMS were compared with those of external standard method to verify the accuracy and applicability of QAMS.[Results]There were no significant differences between the calculated values of QAMS and the measured values of external standard *** relative deviation was less than 3%,and the relative correction factors obtained from the experiment were credible.[Conclusions]QAMS can be used to determine the contents of phenolic acids in Radix salviae Miltiorrhizae formula granules.

关键词： Radix salviae Miltiorrhizae formula granule HPLC QAMS Phenolic acid Relative correction factor

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Effect of Danshen on apoptosis and NF-кB protein expression of the intestinal mucosa of rats with severe acute pancreatitis or obstructive jaundice

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Hepatobiliary & Pancreatic Diseases International 2010年第5期9卷 537-546页

作者： Xi-Ping Zhang Jun Jiang Ya-Ping Yu Qi-Hui Cheng Bin Chen Department of General Surgery Hangzhou First People's Hospital Hangzhou 310006 China. zxp99688@***

BACKGROUND:Intestinal mucosa injury in cases of severe acute pancreatitis(SAP) or obstructive jaundice(OJ) is one of the main reasons for the accelerated aggravation of these *** being an organ to digest and absorb nutrients,the intestine is also a unique immune *** SAP and OJ develop,the destruction of the intestinal mucosa barrier is an important contributing factor for the development of bacterial translocation,systemic inflammatory response syndrome,and multiple organ dysfunction *** is important to protect the intestinal mucosa in the therapy for SAP and *** this study,we determined the effect of Radix salviae Miltiorrhizae(Danshen) injection on apoptosis and NF-κB P65 protein expression in the intestinal mucosa of rats with SAP or OJ,and explored the protective mechanism of Danshen in their ***:Sprague-Dawley rats were used in the SAP and OJ *** rats were randomly divided into shamoperated,model control,and treated *** various times after operation,the mortality rates were ***,the rats were killed to assess the pathological changes,the expression levels of Bax and NF-κB proteins,and the apoptosis indices in the intestinal ***:Compared to the corresponding model control group,the number of SAP or OJ rats that died in the treated group decreased but showed no statistically significant *** all time points after operation,there was no significant difference between the treated and model control groups in the staining intensity as well as the product of staining intensity and positive staining rate of Bax protein in the intestinal mucosa of SAP and OJ *** 3 hours after operation,the apoptosis index of the intestinal mucosa of SAP rats in the treated group was lower than that in the model control group(P<0.01).At 12 hours after operation in SAP rats and 28 days after operation in OJ rats,the staining intensity as well as the product of staining intensity and

关键词： severe acute pancreatitis obstructive jaundice Radix salviae Miltiorrhizae traditional Chinese medicine intestinal mucosa NF-κB apoptosis tissue microarray

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丹参的提取工艺研究

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安徽农业科学 2012年第21期40卷 10873-10875页

作者：贾永艳田效志李九席贾永魏超娟河南中医学院河南郑州450008

[目的]优选丹参的提取工艺条件。[方法]采用均匀试验设计方法,以丹参酮ⅡA、丹酚酸B和总酚酸量为考察指标,优选丹参的超临界萃取及醇提工艺条件。[结果]丹参的最佳超临界萃取条件为:萃取压力25 Mpa,萃取温度40℃,萃取时间2 h,料液比为10... 详细信息

[目的]优选丹参的提取工艺条件。[方法]采用均匀试验设计方法,以丹参酮ⅡA、丹酚酸B和总酚酸量为考察指标,优选丹参的超临界萃取及醇提工艺条件。[结果]丹参的最佳超临界萃取条件为:萃取压力25 Mpa,萃取温度40℃,萃取时间2 h,料液比为10∶3(g/ml);在此条件下,丹参酮ⅡA的转移率为69.03%。丹参的最佳醇提条件为:料液比1∶11(g/ml),乙醇浓度30%,回流3次,每次0.5h。[结论]优化后的工艺合理、稳定、可行,适于丹参的提取。

关键词：丹参(RADIX ET RHIZOMA salviae MILTIORRHIZAE) 丹参酮ⅡA 丹酚酸B 提取工艺均匀试验设计

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PROLONGED PARALYSIS OF CHILDREN TREATED WITH SCOPOLAMINE ACUPOINT INJECTION

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World Journal of Acupuncture-Moxibustion 1993年第1期3卷 27-30,38页

作者： Zhang Xiuhui Xiong Cuifeng Deng Jihua Kong Qingfang Tu Junjie Consultant: Wei Jia, Jiangxi College of TCM, Nanchang, Jiangxi 330006, China

Authors reported 209 cases of childish paralysis. Of them 104 cases were treatedwith scopolamine acupoint injection, and another 105 cases were divided into 3 groups and treatedwith scopolamine intramuscular injection, salviae miltiorrhizae acupoint injection and acupuncture re-spectively as control. The results show that the therapeutic effect of scopolamine acupoint injection ismuch better than that of all other groups.

关键词： Scopolamine salviae Paralysis Acupoint injection

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