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Comparative transcriptome analysis on the alteration of gene expression in ayu （Plecoglossus altivelis）larvae associated with salinity change

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Zoological Research 2016年第3期37卷 126-135页

作者： Xin-Jiang LU Hao ZHANG Guan-Jun YANG Ming-Yun LI Jiong CHEN Laboratory of Biochemistry and Molecular Biology School of Marine Sciences Ningbo University Ningbo 315211 China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo University Ningbo 315211 China

Ayu （Plecoglossus altivelis） fish, which are an amphidromous species distributed in East Asia, live in brackish water （BW） during their larval stage and in fresh water （FW） during their adult stage. In this study, we found that FW-acclimated ayu larvae exhibited a slower growth ratio compared with that of BW-acclimated larvae. However, the mechanism underlying FW acclimation on growth suppression is poorly known. We employed transcriptome analysis to investigate the differential gene expression of FW acclimation by RNA sequencing. We identified 158 upregulated and 139 downregulated transcripts in FW-acclimated ayu larvae compared with that in BW-acclimated larvae. As determined by Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway mapping, functional annotation of the genes covered diverse biological functions and processes, and included neuroendo- crinology, osmotic regulation, energy metabolism, and the cytoskeleton. Transcriptional expression of several differentially expressed genes in response to FW acclimation was further confirmed by real-time quantitative PCR. In accordance with transcriptome analysis, iodothyronine deiodinase （ID）, pro-opiom- elanocortin （POMC）, betaine-homocysteine S-meth- yltransferase 1 （BHMT）, fructose-bisphosphate aldolase B （aldolase B）, tyrosine aminotransferase （TAT）, and Na＋-K＋ ATPase （NKA） were upregulated after FW acclimation. Furthermore, the mRNA expressions of b-type natriurefic peptide （BNP） and transgelin were downregulated after FW acclimation. Our data indicate that FW acclimation reduced the growth rate of ayu larvae, which might result from the expression alteration of genes related to endocrine hormones, energy metabolism, and direct osmoregulation.

关键词： Plecoglossus altivelis salinity change Transcriptome analysis Growth rate Real-time quantitative PCR

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Research on soil salt transfer under freeze-thawing condition

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Global Geology 2011年第2期14卷 106-109页

作者： LIU Lei LI Xin WEN Hao College of Construction Engineering Jilin University Changchun 130026 China Institute of Water Conservancy Survey and Design Changchun 130062 China

Soil salt transformation plays an important role in the freeze-thawing process,which is also one of basic problems of cryopedology. The very special law is made up of the two time salt-moisture transfer under freeze-thawing condition. Based on the latest research at home and abroad,through the investigation of soil moisture-salt change in the freeze-thawing process,the conclusion is made that the soil water potential gradient is the main driving force of soil salt movement and the factors are of quantities. The research shows that,when freezing,temperature drops,salt and moisture move towards frozen layer. All make the salinity content of the frozen layer increase significantly. In the thawing process,salinity and moisture in the soil move up again with evaporation and makes the salt second migration.

关键词： frozen soil salinity change two-time migration soil water potential gradient

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