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Effect of heat treatment on antimycotic activity of sahara honey

Journal of Coastal Life Medicine 2014年第11期 876-881页

作者： Moussa Ahmed Saad Aissat Noureddine Djebli Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem UniversityMostaganemAlgeria

Objective:To evaluate the influence of the temperature on honey colour,polyphenol contents and antimycotic capacity and to evaluate the correlation between these ***:sahara honey were heated up to 25,50,75 and 100℃for 15,30 and 60 min,and their colour intensity,polyphenol contents and antimycotic *** Folin-Ciocalteu test was used to determine the total polyphenol contents(TPC).The antimycotic activity was evaluated both by agar diffusion method and micro wells dilution method against the Candida albicans(***)and Candida glabrata(***).Results:Initial values for TPC in sahara honey ranged from 0.55 to 1.14 mg of gallic acid per kg of honey,with the average value of 0.78 mg of gallic acid per kg of *** TPC values after heat-treatment were 0.54 to 1.54 with the average value of 1.49 *** minimal inhibitory concentrations before heat-treatment of sahara honey against *** and *** ranged from 3.06%-12.5%and 50%*** heat-treatment the minimal inhibitory concentrations between 12.5%and 50%for *** and ***,*** diameters of inhibition zones of sahara honey with 50%concentration varied from(12.67-15.00)mm by *** to(14.33-15.67)mm by *** diameters of inhibition zones after heat-treatment at 25 and 50°C for 15.30 and 60 min ranged from(2.00-18.67)mm by *** to(8.00-16.67)mm by *** significant relations between the TPC and the colour intensity of sahara honey(r=0.99,Psahara honey.

关键词： sahara honey Antimycotic capacity Heat treatment

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Preliminary comparative study of anti-inlfammatory effect of unheated and heat-treated sahara honey:In vivo approach

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Journal of Coastal Life Medicine 2015年第11期 875-878页

作者： Moussa Ahmed Saad Aissat Noureddine Djebli Pharmacognosy and Api-Phytotherapy Research Laboratory Mostaganem UniversityMostaganemAlgeria

Objective:To investigate the effect of unheated and heat-treated of sahara ***:A total of 24 Swiss albino mice weighing 25-35 g were divided into four groups(n=6).Anti-inflammatory effect was assessed at 1,2,3,4,5 and 6 h after subplantar injection of carrageenan(0.5 mL of a 1%solution in normal saline).In addition,total phenolic content was determined by modified Folin-Ciocalteu ***:The total phenolic content capacity of the sahara honey before and after heat treatment was between 72 and 97.9 mg of gallic acid equivalents/100 g of honey *** of unheated honey(oral administration)reduced significantly(Phoney showed insignificant inhibition of carrageenan and induced paw edema at 1 h(31.16%),3 h(0.25%)and 6 h(34.19%).The 50 mg/kg diclofenac exhibited percent reduction in paw volume 16.12%,8.90%and 15.32%after 1 h,3 h and 6 h,respectively,when compared with control *** toxicity was ***:Our results suggest that unheated sahara honey has anti-inflammatory effects by reducing the mice paw edema size while heat-treated sahara honey decreases the antiinflammatory activity.

关键词： sahara honey Heating Anti-inflammatory Total phenolic content

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