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The effect of interaction between Bacillus subtilis DBM and soil minerals on Cu(Ⅱ) and Pb(Ⅱ) adsorption

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Journal of Environmental Sciences 2019年第4期31卷 328-337页

作者： Jun Bai Yuanqing Chao Yanmei Chen Shizhong Wang Rongliang Qiu School of Environmental Science and Engineering Sun Yat-sen University School of Public Health Southwest Medical University Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology Sun Yat-sen University Guangdong Provincial Engineering Research Center for Heavy Metal Contaminated Soil Remediation Sun Yat-sen University

The effects of interaction between Bacillus subtilis DBM and soil minerals on Cu(Ⅱ)and Pb(Ⅱ)adsorption were *** combination with DBM,the Cu(Ⅱ)and Pb(Ⅱ)adsorption capacities of kaolinite and goethite improved compared with the application of the minerals *** modeling results of potentiometric titration data proved that the site concentrations of kaolinite and goethite increased by 80%and 30%,respectively after combination with ***,the involvement of functional groups in the DBM/mineral combinations resulted in lower concentrations of observed sites than the theoretical values and led to the enhancement of desorption rates by NH_4NO_3 and EDTA-Na_*** DBM-mineral complexes might also help to prevent heavy metals from entering DBM cells to improve the survivability of DBM in heavy metal-contaminated *** the combination process,the extracellular proteins of DBM provided more binding sites for the minerals to absorb Cu(Ⅱ)and Pb(Ⅱ).In particular,an especially stable complexation site was formed between goethite and phosphodiester bonds from EPS to enhance the Pb(Ⅱ)adsorption ***,we can conclude that the DBM–mineral complexes could improve the Cu(Ⅱ)and Pb(Ⅱ)adsorption capacities of minerals and protect DBM in heavy metal-contaminated environments.

关键词： Bacillus subtilis DBM Goethite Kaolinite Cu Pb

Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli

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Journal of Animal Science and Biotechnology 2019年第4期10卷 969-980页

作者： Kwangwook Kim Yijie He Xia Xiong Amy Ehrlich Xunde Li Helen Raybould Edward R.Atwill Elizabeth A.Maga Jens JΦrgensen Yanhong Liu Department of Animal Science University of CaliforniaDavisCA 95616USA School of Veterinary Medicine University of CaliforniaDavisCA 95616USA Chr.Hansen A/S HoersholmDenmark

Background: There is growing evidence to support the beneficial effects of supplementing direct-fed microbials(DFM) on performance, health status, and immune responses of weaned pigs. Therefore, the objective of this study was to investigate dietary supplementation of Bacillus subtilis(DSM 25841) on growth performance, diarrhea, gut permeability and immunity of weaned pigs experimentally infected with a pathogenic F-18 Escherichia coli(E. coli).Results: The F18 E. coli infection reduced(P subtilis linearly enhanced average daily gain of E. coli infected pigs from d 0 to 5 post-inoculation(PI)(P subtilis reduced(P subtilis linearly up-regulated(P subtilis to control diet linearly down-regulated gene expression of IL1 A on d 5 PI(P = 0.07) and IL6 on d 11 PI(P subtilis(DSM 25841) enhanced growth r

关键词： Bacillus subtilis Growth rate Gut barrier function Intestinal inflammation Pathogenic Escherichia coli Weaned pigs

Fermentation, formulation and evaluation of PGPR Bacillus subtilis isolate as a bioagent for reducing occurrence of peanut soil-borne diseases

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Journal of Integrative Agriculture 2019年第9期18卷 2080-2092页

作者： Abdel-Gayed M.Ahmad Abo-Zaid G.Attia Matar S.Mohamed Hafez E.Elsayed Onion Garlic and Oil Crops Research DepartmentPlant Pathology Research InstituteAgricultural Research CenterGiza 12619Egypt Bioprocess Development Department Genetic Engineering and Biotechnology Research InstituteCity of Scientific Research and Technological ApplicationsNew Borg El-Arab CityAlexandria 21934Egypt Chemical Engineering Department Faculty of EngineeringJazan UniversityJazan 45142Saudi Arabia Plant Protection and Biomolecular Diagnosis Department Arid Lands Cultivation Research InstituteCity of Scientific Research and Technological ApplicationsNew Borg El-Arab CityAlexandria 21934Egypt

Four isolates of Bacillus subtilis coded,B4,B7,B8 and B10 were examined as biocontrol agents for their abilities and antagonistic effect on the in vitro growth of certain phytopathogenic fungi of peanut,Rhizoctonia solani and Sclerotium *** subtilis isolate B4(GenBank accession ***150884)was the highly effective one for inhibiting the fungal mycelial *** fermentation of *** isolate B4 was carried out and the maximum biomass achieved was 4.53 g L-1 at 11 *** subtilis isolate B4 was formulated and evaluated as a biofungicide to reduce peanut soil-borne diseases under greenhouse and field conditions at the side of Rizolex-T(fungicide)as *** by formulated plant growth-promoting rhizobacteria(PGPR)*** B4 and Rizolex-T in a soil infested with ***,*** and mixture of them were more effective in decreasing percentage of damping-off,root and pod rot disease incidence(%)in greenhouse and open field environment during the two seasons 2015 and *** by PGPR gave highly dry weight and number of healthy pods compared to control of fungi treatment which was nearby to dry weights of healthy pods achieved by treatments by Rizolex-T in a soil infested with ***,*** and mixture of *** PGPR *** B4 gave higher increasing of yield percentage than treatment by Rizolex-T in the two evaluated seasons 2015 and *** can conclude that the produced bioforumlated agent was more efficient as fungicide when compared with the other chemical synthesized fungicides,safe for human and the environment and economy.

关键词： peanut soil-borne diseases Bacillus subtilis biocontrol fermentation formulation

Transcriptional profile of gene clusters involved in the methylerythritol phosphate pathway in Bacillus subtilis 916

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Journal of Integrative Agriculture 2019年第3期18卷 644-655页

作者： XIAO Ya-jing GAO Tan-tan PENG Qi ZHANG Jie SUN Dong-mei SONG Fu-ping College of Life Science and Technology Heilongjiang Bayi Agricultural University State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences

The methylerythritol phosphate pathway is responsible for the biosynthesis of terpenoids, the largest class of secondary metabolites. Although the structures and functions of the proteins involved in this pathway have been well studied in Bacillus subtilis, only a few studies have reported the transcriptional profile of the genes involved. Therefore, we analyzed methylerythritol phosphate pathway genes in the genome of B. subtilis 916, which has been developed as a biological control agent against some rice diseases in China. Our results showed that methylerythritol phosphate pathway genes were distributed throughout the genome of this strain. These genes were transcribed during both the exponential and stationary phases. We further confirmed the transcription units of dxs, dxr, ispD, ispF, ipK, ispG, ispH, idi, and ispA in B. subtilis 916 through reverse transcription-PCR analyses; the results showed that these nine genes were located in seven different operons. The transcript start sites of the seven different operons were determined by 5′-rapid amplification of cDNA ends-PCR. Thus, our study provides a molecular basis at the transcriptional level for investigating homoterpene synthesis in the methylerythritol phosphate pathway of B. subtilis 916.

关键词： methylerythritol phosphate pathway transcription Bacillus subtilis biological control

Influence of nano-MgO particle size on bactericidal action against Bacillus subtilis var. niger

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Chinese Science Bulletin 2005年第6期50卷 514-519页

作者： HUANGLei LIDianqing LINYanjun DavidG.Evans DUANXue KeyLaboratoryofScienceandTechnologyofControllableChemicalReactions MinistryofEducationBeijingUniversityofChemicalTechnologyBeijing100029China

Nano-MgO with various particle sizes, synthe- sized by different methods using Mg(NO3)2·6H2O, Na2CO3, Na2SO4, urea and ammonia solution as reactants, was used to carry out bactericidal experiments on Bacillus subtilis var. niger. The results were compared with the effect of TiO2, a common kind of photocatalytic material. The materials were characterized by X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), low temperature N2 adsorp- tion-desorption measurements and FT-IR, and the results showed that the bactericidal ability of MgO increases with decreasing particle size. Nano-MgO and an interior wall-paint containing the material have better bactericidal effects than nono-TiO2 in both presence and absence of light. The bactericidal mechanism is discussed. The surface of MgO can generate high concentrations of O?2 which is highly active and can react with the peptide linkages in the coating walls of the spores. The spores are destroyed by the resulting damage to their structure.

关键词：氧化镁合成方法 “Bacillus subtilis var.niger” 杀菌活性细菌氧离子晶体

Effect of Saccharomyces boulardii and Bacillus subtilis B10 on gut microbiota modulation in broilers

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Animal Nutrition 2018年第4期4卷 358-366页

作者： Chubin Qin Li Gong Xiaoping Zhang Yuanyuan Wang Yibin Wang Baikui Wang Yali Li Weifen Li Key Laboratory of Molecular Animal Nutrition Ministry of Education College of Animal Science Zhejiang University China National Bamboo Research Center Key Laboratory of High Efficient Processing of Bamboo of Zhejiang Province

The gut microbiota plays important roles in animal overall health and productiveness. Balancing host gut microbiota by probiotics has been documented. Our previous study showed that Saccharomyces boulardii(Sb) and Bacillus subtilis B10(Bs) significantly improve growth performance and modulate the intestinal histomorphology in broilers. To increase the knowledge regarding Sb and Bs, this study investigated the effects of these 2 probiotic strains on the gut microbiota in broilers. Three hundred 1-day-old Sanhuang broilers(Chinese cross breed) were randomly divided into 3 groups, each group with 5 replications(n = 20). The control group(CK) was fed a basal diet containing an antibiotic(virginiamycin, 20 mg/kg) and the other 2 groups received Sb and Bs(1 × 10~8cfu/kg of feed) in addition to the basal diet. After 72 d of treatment, pyrosequencing revealed that the bacterial communities varied along the section of intestinal tract in the control and Bs groups, but not in the Sb group. No difference in microbial diversity was observed among 3 groups. The major phyla observed along the GI tract of broilers(particularly in the duodenum and cecum) were Firmicutes, Bacteroidetes, Proteobacteria, and Verrucomicrobia, which were considered potentially growth performance-related. Bacteroidetes, Proteobacteria, and Verrucomicrobia were observed at a much higher abundance in the jejunums and ileums of the Sb group(P < 0.05). In addition, the jejunal microbial communities formed 3 different clusters at either the genus level or the category of metabolism among the groups, based on the principal component analyses. These data indicated that Sb and Bs can modulate the microbial ecosystem, and subsequently enhance the health status of broilers.

关键词： Saccharomyces boulardii Bacillus subtilis B10 Gastrointestinal tract Microbiota Broiler

Spontaneous cerebral abscess due to Bacillus subtilis in an immunocompetent male patient: A case report and review of literature

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World Journal of Clinical Cases 2018年第16期6卷 1169-1174页

作者： Ioannis Tsonis Lydia Karamani Panagiota Xaplanteri Fevronia Kolonitsiou Petros Zampakis Georgios Gatzounis Markos Marangos Stelios F Assimakopoulos Department of Neurosurgery University of Patras Medical School Department of Microbiology University of Patras Medical School Department of Radiology University of Patras Medical School Department of Internal Medicine Division of Infectious Diseases University of Patras Medical School

BACKGROUND Bacillus subtilis(B. subtilis) is considered a nonpathogenic microorganism of the genus Bacillus and a common laboratory contaminant. Only scarce reports of B. subtilis central nervous system infection have been reported, mainly in the form of pyogenic meningitis,usually in cases of direct inoculation by trauma or *** SUMMARY A 51-year-old man, with a free previous medical history, presented to the Emergency Department of our hospital complaining of recurrent episodes of left upper limb weakness, during the last month, which had been worsened the last 48 h. During his presentation in Emergency Department he experienced a generalized tonic-clonic grand mal seizure. Brain magnetic resonance imaging(MRI) scan with intravenous Gadolinium revealed a 3.3 cm × 2.7 cm lesion at the right parietal lobe surrounded by mild vasogenic edema, which included the posterior central gyrus. The core of the lesion showed relatively homogenous restricted diffusion. Post Gadolinium T1 W1 image, revealed a ring-shaped enhancement. Due to the imaging findings, brain abscess was our primary consideration. Detailed examination for clinical signs of infectious foci revealed only poor oral hygiene with severe tooth decay and periodontal disease, but without detection of dental abscess. The patient underwent surgical treatment with right parietal craniotomy and total excision of the lesion. Pus and capsule tissue grew B. subtilis and according to antibiogram intravenous ceftriaxone 2 g bids was administered for 4 wk. The patient remained asymptomatic and follow-up MRI scan two months after operation showed complete removal of the *** This case highlights the ultimate importance of appropriate oral hygiene and dental care to avoid potentially serious infectious complications and second, B. subtilis should not be considered merely as laboratory contaminant especially when cultivated by appropriate central nervous system specimen.

关键词： Bacillus subtilis Brain abscess Central nervous system infection Craniotomy Meningitis Case report

Identification of a disulfide bonded protein from cytoplasm of Bacillus subtilis

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Chinese Science Bulletin 1997年第20期42卷 1756-1760页

作者： Wang, T Kadokura, H Yamasaki, M Chinese Acad Sci Inst Bot Beijing 100095 Peoples R China

DISULFIDE bonds are important for some proteins to form stable secondary or tertiary conforma-tion. These known disulfide bonded proteins are mainly present in periplasm or cel1ular or-ganelles, most of which are secreted proteins or membrane proteins. It is generally recog-nized that cytoplasmic proteins do not have disulfide bonds because of reducing environment

关键词： Bacillus subtilis cytoplasm disulfide bonded protein.

NMR studies of the interaction between Bacillis subtilis neutral proteinase and inorganic metal compounds

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Chinese Science Bulletin 2000年第19期45卷 1764-1766页

作者： ZHENG Xuefang HU Jiehan XU Yongting CHENG Guobao GAI Hongwei Deparlmenl of Chemistry and Chemical Engineering Dalian UniversityDalian 116622China Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023China Experimental Centre of Liaoning Normal University Dalian 116021China

The interaction between Bacillis subtilis neutral proteinase (***) and inorganic metal compounds (CoCI2, NiCI2) was investigated by 1H NMR spectroscopy. It has been shown that the Zn(Ⅱ) ion in the active center of the native enzyme may directly interact with external CoCI2 and NiCI2, producing Co(Ⅱ)- and Ni(Ⅱ)-substituted derivatives, and their 1H NMR spectra were obtained for the first time. From the 1H NMR spectra, the coordinated structure of the active center in the native enzyme was described.

关键词： Bacillis subtilis neutral proteinase CoCl2 NiCl2 interaction NMR.