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Analysis on Genetic Differences between Parents of Two-line Medium Indica Hybrid Rice by ssr markers

Agricultural Science & Technology 2017年第10期18卷 1800-1804页

作者： Shilong XU Tianze YAN Richao ZHONG Peng QIN Kai WANG Yuanzhu YANG College of Agronomy Hunan Agricultural University Changsha 410128 China Hunan Longping High-tech Seed Science Research Institute Co. Ltd. Changsha 410119 China Hunan Engineering Laboratory for Disease and Pest Resistant Rice Breeding Yuan Longping High-tech Agriculture Co. Ltd Changsha 410119 China Hunan University of Humanities Science and Technology Loudi 417000 China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research Center Changsha 410125 China

The genetic diversity of 50 parents or improved lines of two-line medium Indica hybrid rice were analyzed based on 48 pairs of ssr markers. The results showed that the clustering results were highly accordant with the parental pedigree information of the tested materials. The male sterile lines and the restoring lines were clustered into 2 distinct groups （Ⅰ and Ⅱ）, respectively. There were no significant difference in intra-group average genetic distances （GD） between the 2 groups. Most of the male sterile lines from Hunan Longping High-tech Seed Science Research Institute Co., Ltd. were grouped into a single subgroup （Ⅰ-4） under the male sterile group with the largest average GD compared with the rest male sterile subgroups. Huazhan derived lines and Guangzhou inbred lines were founded to be independent of 9311 and its derivatives or closely-related lines. Meanwhile, they have obviously been differentiated into 2 subgroups （Ⅱ-2 and Ⅱ-3）. The lines derived from Huazhan （or genetic closely related lines） showed a genetic homogenization phenomenon. Therefore, the Huazhan-derived lines should be further improved by the utilization of germplasms with good quality, high resistance and wide adaptability from Southern China to widen the GD between Huazhan derived lines and the male sterile subgroup developed by Hunan Longping High-tech Seed Science Research Institute Co., Ltd., so as to further promote the heterosis level between the groups.

关键词： Two-line hybrid rice Parent ssr marker

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Physiological Character and Gene Mapping in a New Green-revertible Albino Mutant in Rice

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Journal of Genetics and Genomics 2007年第4期34卷 331-338页

作者：陈涛张亚东赵凌朱镇林静张所兵王才林南京农业大学农学院南京210095 江苏省农业科学院粮食作物研究所江苏省优质水稻工程技术研究中心南京210014

A green-revertible albino mutant-Qiufeng M was found from the japonica rice （Oryza sativa L. ssp. japonica） Qiufeng in the field. The first three leaves of the mutant were albino with some green. The leaf color became pale green since the fourth leaf and the glume had the same phenomenon as the first three leaves. The measuring data of the pigment content confirmed the visually observed results. It truly had a remarkable changing process in the leaf color in Qiufeng M. Comparison of the main agronomic characters between Qiufeng and Qiufeng M indicated that the neck length and grain weight showed significant difference at the 1% level, and other characters were not different. Genetic analysis showed that the green-revertible albino trait was controlled by a single recessive nucleic gene. Using 209 recessive mutant individuals in the F2 population derived from the cross Pei＇ai 64S × Qiufeng M, a gene, tentatively named gra（t）, was located between the ssr markers of RM475 and RM2-22 on the long arm of chromosome 2. The genetic distance were 17.3 cM and 2.9 cM respectively.

关键词： rice green-revertible albino mutant gene mapping ssr marker

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Genetics and Molecular marker Identification of a Resistance to Glomerella Leaf Spot in Apple

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Horticultural Plant Journal 2016年第3期2卷 121-125页

作者： LIU Yuanxia LI Baohua WANG Caihong LIU Chunxiao KONG Xianghua ZHU Jun DAI Hongyi Horticulture & Landscape College Hunan Agricultural University Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants College of HorticultureQingdao Agricultural University College of Agronomy and Crop Protection Qingdao Agricultural University

Apple Glomerella leaf spot(GLS) is a destructive fungal disease that damages apple leaves during the summer in *** new diseaseresistant varieties is considered to be the best way of controlling GLS.A genetic study of resistance to Glomerella leaf spot(GLS) in apple was conducted by using four F_1 hybrid groups(‘Fuji' ×‘Golden Delicious',‘Golden Delicious' ×‘Fuji',‘Gala' ×‘Fuji',and ‘Fuji' ×‘QF-2') generated from two highly resistant varieties or selections,‘Fuji' and ‘QF-2',and two highly susceptible varieties,‘Golden Delicious' and ‘Gala'.The results showed that the separation ratios of resistant plants to the susceptible ones in the four F_1 hybrid groups were statistically consistent with the theoretical ratios of 1:1,1:1,0:1,and 1:*** analysis enabled us to generate the following conclusions:GLS resistance in apple may be controlled by a single recessive *** genotype of the resistant plants was rr,whereas the genotypes of the susceptible ones were RR and *** using ‘Golden Delicious' ×‘Fuji' F_1 hybrid groups and the bulked segregation analysis(BSA) method,the marker S0506206-243 bp associated with disease resistance character to GLS was identified through screening 500 ssr primers encompassing the entire apple genome with even coverage,and the genetic distance between the marker and the GLS resistance gene was 9.8 cM.

关键词： apple Glomerella leaf spot inheritance of resistance ssr marker

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维普期刊数据库

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Review of the Methods for Developing ssr Molecular markers

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Journal of Northeast Agricultural University(English Edition) 2008年第1期15卷 62-66页

作者： ZHAO Xue CHANG Wei HAN Yingpeng LI Wenbin Soybean Research Institute Key Laboratory of Soybean Biology of Chinese Education Ministry Northeast Agricultural University Harbin 150030China

Microsatellite marker （or Simple Sequence Repeate, ssr） is a marker technology based on DNA molecular length poly morphism. It is also one of the most commonly used molecular markers. Traditional ssr marker development methods are relatively time-consuming and mostly relying on the known genome sequence information while recently developed methods of ssr marker based on RAPD, Issr-PCR ssr, the use of hybrid options, sequence tag ssr library access and screening EST-ssr have been widely used. This paper gave an overview of the methods mentioned above for the development of ssr markers.

关键词： ssr marker development method EST-ssr

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QTL mapping of adult-plant resistance to leaf rust in Romanian wheat line Fundulea 900

QTL mapping of adult-plant resistance to leaf rust in Romani...

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中国植物病理学会2015年学术年会

作者： ZHANG Pei-pei LI Qing-luo WANG Xin-hai LI Zai-feng LIU Da-qun College of Plant Protection Hebei Agricultural UniversityBiological Control Center for Plant Diseases and Plant Pests of Hebei

Wheat leaf rust is a destructive worldwide foliar disease of common wheat(Triticum aestivum L.).The most effective,economic means to control the disease is planting resistant cultivars with adult plant resistance(APR).The Romanian wheat line Fundulea 900 showed high leaf rust resistance in the *** identify leaf rust APR genes in Fundulea 900,the mapping populations with 188 Flines from the cross Fundulea 900/Thatcher was phenotyped for leaf rust severities during the2010-2011,2011-2012 and 2012—2013 cropping seasons in the field at Baoding,Hebei province.A total of 1,215 ssr markers were used to identify the quantitative trait loci(QTL) for leaf rust APR in the *** the Fundulea 900/Thatcher population,three QTL were detected,and designated as ***-1BL,***-2DS and *** on chromosome positions and molecular marker tests,***-lBL is Lr46,and ***-TDS is ***-2DS mapped on chromosome 2DS might be a new *** QTL and their closely linked markers are potentially useful for improving leaf rust resistance in wheat breeding.

关键词： Triticum aestivum Puccinia triticina APR ssr marker

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Genetic Analysis and Taste Quality of Main Conventional Japonica Rice Varieties Grown in Jiangsu Province

Genetic Analysis and Taste Quality of Main Conventional Japo...

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江苏省遗传学会2016年学术年会

作者： Hongbing Gong Shengyuan Zeng Chuang Li Shimin Zuo Dedao Jing Tianzi Lin Zongxiang Chen Yafang Zhang Huafei Qian Bo Yu Shenglan Sheng Xuebiao Pan Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of Ministry ofEducationYangzhou University Zhenjiang Agricultural Research Institute

Fifty indica-japonica specific ssr markers were employed to genotype 86 conventional japonica rice cultivars mainly planted in Jiangsu province and 41 loci showed polymorphism among *** amplifying in 86 rice varieties,a total of 113 alleles were detected at the 41 ssr loci,and the allele number ranged from two to six,with an average of 2.76 in each *** information content（PIC） value of these 41 ssr markers ranged from 0.02 to 0.84,with an average value of 0.48;Nei’s expected heterozygosity value of the 41 markers ranged from 0.02 to 0.68,and the average value was *** on the coefficient index ranged from 0.72 to 1.00,we divided the 86 cultivars into three groups by *** analysis of taste quality traits investigated under different environments indicated that the taste quality of conventional japonica rice cultivars in Jiangsu was correlated with the genetic *** long-day（LD） condition,the amylose content（AC） and protein content（PC） manifested a tendency of group I ＞ group II ＞ group III,while the taste value（TV） was *** under short-day（SD） condition,taste quality traits of group II and group III varied significantly compared with that under LD condition,while the traits of group I had no significant *** results indicated that the main cultivars grown in Jiangsu have a general taste quality and narrow genetic background,broadening the genetic basis is an important approach to breed japonica rice cultivars with environment insensitivity and good eating quality.

关键词： ssr marker Japonica rice Taste quality Genetic basis

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Development and QTL analysis of chromosome segment substitution lines derived from backcross indica recipient ‘93-11’ and japonica donar ‘Nipponbare’

Development and QTL analysis of chromosome segment substitut...

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江苏省遗传学会第七届二次代表大会暨学术研讨会

作者： Wenyin Zhu, Dewei Yang, Jing Lin, Ling Zhao, Yadong Zhang, Zhen Zhu, Tao Chen, Cailin Wang (Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Jiangsu High Quality Rice Research and Development Center, Nanjing 210014, China)

＜正＞Most traits for crop breeding are quantitative in nature, which are controlled by polygenes. It is very important for crop improvement to accurately identify and finely map quantitative trait loci

关键词： Rice Chromosome segment substitution lines ssr marker

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Cloning and Furictional Verification of Major Genes in Wheat Grain Number

Cloning and Furictional Verification of Major Genes in Wheat...

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第十届全国小麦基因组学及分子育种大会

作者： Liuping Chen Qiaoyan Chen Wen-hui Wei Life of Science and Technology Henan Institute of Science and Technology

Wheat is one of the world’s major food crops,with approximately35-40% of the world’s population being *** order to meet the demand for food in the future population increase,it is necessary to increase the output per unit *** the number of spikes per unit area reaches a certain range,the yield increase effect has reached a critical *** to the characteristics of the variety and environmental conditions,the increase in grain weight is *** number of kernels per spike is the most active factor in the yield component and can be adjusted to a greater ***,when wheat yield reaches a high level,increasing the number of wheat spikes becomes the main limiting factor for further increasing wheat *** this experiment,the F and low-generation backcross populations of Xianyang chaodasui and Baiqi Mai were *** target major QTL was identified by ssr marker combined with BSA method and wheat 660 K chip,and the molecular interval of the main QTL was *** near-isogenic line of the genetic locus uses the method of map-based cloning to clone the key genes controlling the number of kernels,and validates its biological function by OE and RNAi methods,and studies the expression of key genes in multi-peripheral number *** patterns and genetic interactions provide a theoretical basis for subsequent wheat new germplasm innovation and high-yield wheat breeding.

关键词： Spike grain number QTL ssr marker functional verification

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Development of Simple Sequence Repeat markers Based on Whole Genome Sequencing to Reveal the Genetic Diversity of Glomerella cingulate in China

Development of Simple Sequence Repeat Markers Based on Whole...

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中国植物病理学会2017年学术年会

作者： Liu Zhaotao Lian Sen Li Baohua Dong Xiangli Wang Caixian Cho Wonkyong Liang Wenxing College of Crop Protection Qingdao Agricultural Universityand Key Lab of Integrated Crop Pests Management of Shandong Province Department of Agricultural Biotechnology Research Institute of Agriculture and Life Sciencesand Plant Genomics and Breeding InstituteCollege of Agriculture and Life SciencesSeoul National University

Glomerella Leaf Spot(GLS) has been in an outbreak since 2011 and severely affects apple production(Malus domestica Borkh.) in *** pathogen that causes GLS is Glomerella cingulata(Stoneman) Spauld.& ***,which is epidemic in regions with humid climates from May to October each *** spreads more than 200 km per year,and most apple-producing areas have been invaded in the five years since *** investigate the origin,evolution and diversity of *** for better GLSmanagement,we performed whole genome sequencing of *** isolate 030206 and developed 25 simple sequence repeat marker(ssr) markers according to the ssr motifs identified in the genome *** the 25 ssrmarkers,we analyzed thegenetic diversity and population genetic structure of 96 *** isolates collected from eight prevalent GLS areas;Chiping,Feicheng,Haiyang,Muping,Qixia,Yishui,Yiyuan,and Zhanhua in Shandong province,*** expected heterozygosities(H) for the populations were 0.351,0.240,0.471,0.559,0.556,0.077,0.452,and 0.036,respectively,with a mean of 0.343,and the Shannon indices(I) were 0.560,0.385,0.811,1.100,1.032,0.140,0.662,and 0.051,respectively,with a mean of *** gene flow index(Nm) was *** 96 *** isolates were divided into three subpopulations,denoted GC1,GC2 and ***1 contained 32 isolates,most of which were from the western areas of Shandong province,whereas GC2 contained 18 isolates and GC3 contained 46 isolates,most of which were from the eastern areas of Shandong *** addition,the western genetic population,GC1,contained a few isolates from eastern collection sites,such as Haiyang,Muping and Qixia,whereas few isolates contained in GC2 and GC3 were from western *** results indirectly show that the GLS pathogens in western areas were originally from eastern areas of Shandong province.

关键词： Glomerella cingulata Glomerella Leaf Spot ssr marker Population genetic

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Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16

Molecular mapping of leaf rust resistance gene LrBi16 in Chi...

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2010中国作物学会学术年会

作者： Zhou Yue~1,Zhang Hai~1,Xia Xianchun~2,He Zhonghu~(2,3),Li Xing~1,Li Zaifeng~(1*),Liu Daqun~(1*) (1 College of Plant Protection,Agricultural University of Hebei/ Biological Control Center for Plant Diseases and Plant Pests of Hebei Province,Baoding 071001,China 2 Institute of Crop Science,National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement,Chinese Academy of Agricultural Sciences (CAAS),Beijing 100081,China 3 International Maize and Wheat Improvement Center(CIMMYT) China Office,c/o CAAS,Beijing 100081,China)

Leaf rust,caused by Puccinia triticina,is one of the most widespread diseases in common wheat (Triticum aestivum L.) *** the objective of identifying and mapping new genes for resistance to leaf rust,F,F plants and F lines from a cross between resistant cultivar Bimai 16 and susceptible cultivar Thatcher were inoculated with Chinese Puccinia triticina pathotypes FHTT and PHTS in the *** the first seedling test,Bimai 16,Thatcher,20 F plants,359 F plants and 298 F lines were inoculated with pathotype FHTT.A set of 1,255 ssr primers pairs were used to test the parents,

关键词： Common wheat Triticum aestivum ssr marker Puccinia triticina brown rust

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