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Comparison of Genetic Diversity Between Local Cultivated Aromatic and Non-aromatic Rice in Yunnan Province

Agricultural Science & Technology 2009年第1期10卷 56-59页

作者：白现广程在全蔺忠龙吕广磊黄兴奇云南大学生命科学学院云南昆明650091 云南省农业科学院生物技术与种质资源研究所云南昆明650223 云南农业大学农业与生物技术学院云南昆明650201

[ Objective] The genetic diversity of the local cultivated aromatic rice and non-aromatic rice in Yunnan Province were compared to provide further genetic resources for breeding practice. [Method] Genetic diversity of 10 aromatic rice and 45 non-aromatic rice were analyzed by 64 ssr primers covered on 12 rice chromosomes. [ Result] Per locus 5.44 and 7.98 alleles in average were detected, ranging from 2 to 12 and from 2 to 17 in aromatic and non-aromatic rice, respectively. Average genetic multiplicity index（Hs） was 0.46 and 0.67 respectively. The average polymorphism information content （PIC） was 0.43 and 0.58 in aromatic and non-aromatic rice respectively. [ Conclusion] The results indicated that genetic diversity was higher in non-aromatic rice than in aromatic rice.

关键词： Aromatic rice Local cultivated rice ssr markers Cluster analysis Genetic diversity

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Identification and mapping of a new powdery mildew resistance allele in the Chinese wheat landrace Hongyoumai

Identification and mapping of a new powdery mildew resistanc...

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第八届全国小麦基因组学及分子育种大会

作者： Bisheng Fu Zhiliang Zhang Qiaofeng Zhang Xiaoyou Wu Jizhong Wu Shibin Cai Institute of Crop Germplasm and Biotechnology Jiangsu Academy of Agricultural Sciences/Jiangsu Province Platform for Conservation and Utilization of Agricultural Germplasm Nanjing Agricultural University

Powdery mildew(Pm) caused by Blumeria graminis f ***(Bgt) is one of the world’s major wheat diseases and results in large grain yield *** discovery and utilization of Pm resistance genes is the most common strategy for wheat Pm ***,a wheat landrace from Henan Province in China,is an excellent source of resistance against *** order to identify its Pm inheritance,we set up a segregating population and carried out genetic analysis,which showed that a single recessive resistance gene confers Pm resistance in *** have temporarily named this gene *** marker analysis,chromosomal location,resistance spectrum analysis,and an allehsm test showed that pmHYM is tocated on the long arm of chromosome 7 B(7 BL) and is most likely a new recessive resistance gene allelic with Pm5 e and *** using 90 Kb SNP sequence in the BLASTn analysis against the wheat 7 BL genome sequence,twelve new simple sequence repeat(ssr) markers linked with pmHYM were developed to map pmHYM co-segregating with marker Xmp1207 and between markers Xmp925 and Xmp1158,at genetic distances of 2.8 cM and 2.7 cM,*** addition,physical mapping of the markers linked with pmHYM using Chinese Spring deletion lines indicated that it resides in the 0.86-1.00 bin of 7 BL.

关键词： Powdery mildew Molecular mapping ssr markers pmHYM

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Identification and mapping of a new powdery mildew resistance allele in the Chinese wheat landrace Hongyoumai

Identification and mapping of a new powdery mildew resistanc...

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江苏省遗传学会2017年学术研讨会——“技术创新与遗传学发展”

作者：付必胜 Zhiliang Zhang Qiaofeng Zhang Xiaoyou Wu 吴纪中 Shibin Cai Jiangsu Academy of Agricultural Sciences/The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm Nanjing Agricultural University

Powdery mildew(Pm) caused by Blumeria graminis f. sp. tritici(Bgt) is one of the world’s major wheat diseases and results in large grain yield losses. Discovery and utilization of Pm resistance genes constitutes the most common strategy for wheat Pm control. Hongyoumai, a wheat landrace from Henan Province in China, has excellent resistance to infection by Bgt. In order to identify the basis of such Pm resistance, a segregating population was submitted to genetic analysis, which showed that Pm resistance in Hongyoumai was conferred by a single recessive resistance gene. This gene was temporarily named pmHYM. Molecular marker analysis, chromosomal location, resistance spectrum analysis and an allelism test showed that pmHYM was located on the long arm of chromosome 7 B(7 BL), most likely representing a new recessive resistance gene allelic with Pm5 e and mlXBD. By using 90 kb single nucleotide polymorphisms sequences(SNP) in the BLASTn analysis against the wheat 7 BL genome sequence, twelve new simple sequence repeat(ssr) markers linked with pmHYM were developed to map pmHYM co-segregating with the marker Xmp1207 and between markers Xmp925 and Xmp1158, at genetic distances of 2.8 c M and 2.7 cM, respectively. In addition, physical mapping of the markers linked with pmHYM using Chinese Spring deletion lines indicated a location in the 0.86–1.00 bin of 7 BL.

关键词： powdery mildew Chinese wheat landrace molecular mapping ssr markers pmHYM

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73个我国育成的紫肉甘薯品种指纹图谱构建（英文）

73个我国育成的紫肉甘薯品种指纹图谱构建（英文）

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2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者：高闫飞苏在兴后猛唐维刘旋旋李强江苏徐淮地区徐州农业科学研究所/农业部甘薯生物学与遗传育种重点实验室/中国农业科学院甘薯研究所江苏师范大学生命科学学院

Purple-fleshed sweetpotatoes(PFSP) are not only rich in anthocyanin, but also edible and processed. Many researchers have proved that anthocyanins have strong antioxidant properties and can prevent aging and increase human immunity effectively. In the past 20 years, more than 70 PFSP cultivars have been released in China. In order to enhance the PFSP breeding efficiency and improve PFSP breeding, the genetic diversity of PFSP reseased in China should be studied and a fingerprint of PFSP should be built. Simple sequence repeat(ssr) markers have been shown to be a powerful tool for varieties identification and fingerprinting in plants. In this study, a total of 124 ssr primer pairs were used to analyze diversity of PFSP, and two highly polymorphic ssr primer pairs(ssr15 A and ssr63 A) showed good polymorphism. Two primer pairs of ssr were used to amplify 73 PFSP, and 23 polymorphic bands(92%) were gotten. ssrbased Genetic similarity coefficients varied from 0.6393-0.9262,with an average of 0.7827 among these varieties. Thus, these PFSP varieties exhibited high levels of genetic similarity and had distinct fingerprint profiles. The ssr fingerprints of the 73 PFSP had been constructed successfully by use 2 highly polymorphic ssr primer pairsThe two highly polymorphic primer pairs developed in this study have the potential to be used as core primer pairs for variety identification and linkage map construction in PFSP or other sweetpotato varieties.

关键词： Sweetpotato Purple flesh ssr markers Fingerprinting

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