检索结果-南通市图书馆

NOVA1 promotes smn2 exon 7 splicing by binding the UCAC motif and increases smn protein expression

Neural Regeneration Research 2022年第11期17卷 2530-2536页

作者： Li-Li Du Jun-Jie Sun Zhi-Heng Chen Yi-Xiang Shao Liu-Cheng Wu Institute of Comparative Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-Innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina Laboratory Animal Center Nanjing University of Chinese MedicineNanjingJiangsu ProvinceChina Institute of Neuroscience Soochow UniversitySuzhouJiangsu ProvinceChina

Spinal muscular atrophy(SMA)is a rare hereditary neuromuscular disease with a high lethality rate in *** in the homologous genes survival of motor neuron(smn)1 and smn2 have been reported to be SMA pathogenic *** studies showed that a highinclusion rate of smn2 exon 7 increased smn expression,which in turn reduced the severity of *** inclusion rate of smn2 exon 7 was higher in neural tissues than in non-neural ***-oncological ventral antigen(NOVA)is a splicing factor that is specifically and highly expressed in *** plays a key role in nervous system development and in the induction of nervous system *** r,it remains unclear whether this splicing factor affects *** this study,we analyzed the inclusion of smn2 exon 7 in different tissues in a mouse model of SMA(genotype smn^(-/-)smn2^(2 tg/0))and litter mate controls(genotype smn^(+/-)smn2^(2 tg/0)).We found that inclusion level of smn2 exon 7 was high in the brain and spinal co rd tissue,and that NOVA1 was also highly expressed in nervous system *** addition,smn2 exon 7 and NOVA1 were expressed synchronously in the central nervous *** further investigated the effects of NOVA1 on disease and found that the number of neurons in the anterior horn of spinal cord decreased in the mouse model of SMA during postnatal days 1-7,and that NOVA1 expression levels in motor neurons decreased simultaneously as spinal muscular atrophy *** also found that in vitro expression of NOVA1 increased the inclusion of smn2 exon 7 and expression ofthe smn2 protein in the U87 MG cell line,whereas the opposite was observed when NOVA1 was knocked ***,point mutation and RNA pull-down showed that the UCAC motif in smn2 exon 7 plays a critical role in NOVA1 binding and promoting the inclusion of exon *** r,CA was more essential for the inclusion of exon 7 than the order of Y residues in the ***,these findings indicate that NOVA1 intera

关键词： exon 7 inclusion motor neuron neuro-oncological ventral antigen 1 smn2 splicing spinal cord spinal muscular atrophy splicing factors UCAC motif

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：