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2012国际生理学学术大会

作者： Yan Z. Wu L. Xue D.D. Chen W.F. Department of Physiology Medical College of Qingdao University

＜正＞Both ginsenoside Rg1 and insulin-like growth factor-I（IGF-I） has been reported to exert neurotropic and neuroprotective effects on the nervous *** present study was designed to investigate the neuroprotective effects of Rgl,IGF-I or Rg1 combined with IGF-I on 6-OHDA-induced neurotoxicity in sk-n-sh *** with Rgl or IGF-I significantly increased the cell viability against 6-OHDA-induced *** Rgl or IGF-I could attenuate 6-OHDA-induced decrease in mitochondrial membrane potential.6-OHDA-induced up-regulation of Bax protein expression could be restored by Rgl or IGF-I treatment. Cotreatment with Rgl and IGF-I resulted in an enhancement of cell *** results suggest that ginsenoside Rgl and IGF-I have neuroprotective effects against 6-OHDA-induced apoptosis in sk-n-sh *** with Rgl and IGF-I have synergistic effect on cell survival in sk-n-sh cells.

关键词： ginsenoside Rg1 6-OHDA sk-n-sh cells insulin-like growth factor-Ⅰ

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Molecular characterization and functional expression of opioid receptor-libe1 receptor

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Cell Research 1997年第1期7卷 69-77页

作者： WU YA LAn LU PU KUn LInG JIAn ZHAO ZHI JIE CHEnG LAn MA GAnG PEI(shanghai Institute of Cell Biology, Chinese Academy of Sciences, shanghai 200031, China)(shanghai Medical University, shanghai 200032, China) shanghai Medical University Shanghai 200032 China

The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DnAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demonstrated that ORL expressed in human neuroblastoma sk-n-sh and sh-SY5Y cell lines by radioligand binding assay, reverse transcription polymerase chain reaction (RT-PCR) and northern analysis in the present study. Stimulation with ORL1 specific agonist, nociceptin/orphanin Fo, increased [34S]GTPrγS binding to sk-n-sh cell membranes (EC50 = 14 ±0.45 nM), and attenuated forskolin-stimulated accumulation of cellular cAMP (EC50= 0.80 ±0.45 nM, indicative that activation of ORL1 activates G proteins and inhibits adenylyl cyclase. Activation of ORL1 receptor was also accessed using CHO:hORL1 cell line by microphysiometer. Treatment of nociceptin/orphanin FQ increased extracellular acidification rate significantly.

关键词： Opioid receptor-libe1 receptor (ORL1) nociceptin/orphanin FQ (n/OFQ) sk-n-sh cells extracellular acidification functional expression

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