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The effect of vitamin D on sperm motility and the underlying mechanism

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Asian Journal of Andrology 2019年第4期21卷 400-407页

作者： Kadiliya Jueraitetibaike Zheng Ding Dan-Dan Wang Long-Ping Peng Jun Jing Li Chen Xie Ge Xu-Hua Qiu Bing Yao Center of Reproductive Medicine Nanjing Jinling Hospital Clinical School of Medical College Nanjing University Nanjing 210002 China The Affiliated Jiangning Hospital of Nanjing Medical School Nanjing 210000 China Urology Department Shunde Hospital of Southern Medical University Foshan 528300 China

Vitamin D deficiency is a common health issue around the world. We therefore evaluated the associations of semen quality with both serum and seminal plasma vitamin D levels and studied the mechanisms underlying these by incubating spermatozoa with 1,25(OH)2D in vitro. Two hun dred and twenty-two men were in eluded in our study. Vitamin D was detected using an electrochemilumi nesce nee method. Spermatozoa used for in vitro experiments were isolated by density gradient centrifugation. Positive relationships of serum 25(OH)D with semen volume and seminal plasma fructose were identified. Seminal plasma 25(OH)D level showed no relationship with serum 25(OH)D level, while it was inversely associated with sperm concentration and positively correlated with semen volume and sperm kinetic values. In vitro, sperm kinetic parameters in creased after in cubation with 1,25(OH)2D, especially upon in cubation for 30 min with it at a concen tration of 0.1 nmol l-1. Under these in cubation conditions, the upward migratio n of spermatozoa in creased remarkably with increasing ade nosine triphosphate (ATP) con centratio n. The concentrati on of cyclic ade nosine mono phosphate (cAMP) and the activity of protei n kinase A (PKA) were both elevated, and the PKA inhibitor, N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89) reversed the in crease of ATP producti on. The conce ntrations of cytoplasmic calcium ions and n icotinamide adenine dinucleotide (NADH) were both enhanced, while mitochondrial calcium uniporter (MCU) inhibitor, Ruthenium 360 (Ru360) did not reverse the increase of ATP production. Therefore, seminal plasma vitamin D may be invoIved in regulating sperm motility, and 1,25(OH)2D may enhance sperm motility by promoting the synthesis of ATP both through the cAMP/PKA pathway and the in crease in in tracellular calcium ions.

关键词： ade no sine triphosphate semi nal plasma sperm motility vitamin D

基于sines分子标记的拟南芥不同生态型的鉴定与划分

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基于SINEs分子标记的拟南芥不同生态型的鉴定与划分

作者：赵宁西藏大学

学位级别：硕士

我们首次在被称为“世界第三极”的青藏高原发现植物研究的模式种拟南芥。本次研究首先在拟南芥发现的基础上探讨了关于生态型鉴定和划分的方法;并根据现有方法从表型、海拔、系统进化树和sines分子标记等方面提出在青藏高原发现的拟南... 详细信息

我们首次在被称为“世界第三极”的青藏高原发现植物研究的模式种拟南芥。本次研究首先在拟南芥发现的基础上探讨了关于生态型鉴定和划分的方法;并根据现有方法从表型、海拔、系统进化树和sines分子标记等方面提出在青藏高原发现的拟南芥很有可能是一种新的拟南芥生态型。其次在对拟南芥西藏个体全基因组测序的基础上以Col为参考,提取拟南芥西藏个体的131个sines序列,统计出在已知全基因序列的20个拟南芥生态型中的每个sine的存在和缺失,筛选出至少在一个拟南芥生态型中有缺失的40个sines。并以此为基础,筛选出两组sines的组合可以将这20个拟南芥生态型区分开。最后在全基因组测序的基础上,针对全基因组测序结果中缺失或存在gap的30个sines以Can或Bur的全基因组序列中的sines为模板,在sine前后100-800bp范围内设计引物,扩增出相应的片段并送测序。拿到的测序结果与全基因组测序结果进行比较,进一步验证全基因组测序的准备性并获得全基因测序未读到的序列信息。

关键词：拟南芥 sine 生态型

Au Elements and Their Evolution in Some AIIopolyploid Genomes of Aegilops

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Agricultural Sciences in China 2006年第12期5卷 895-904页

作者： GONG Han-yu WANG Jian-bo Key Laboratory of MOE for Plant Developmental Biology/College of Life Science Wuhan University Wuhan 430072 P.R. China College of Life Sciences South-Central University for Nationalities Wuhan 430074 P.R.China

To study the sequences of short interspersed nuclear elements （sines） evolution in some allopolyploid genomes of Aegilops, 108 Au element fragments （a novel kind of plant sine） were amplified and sequenced in 10 species of Aegilops, which were clustered into three different groups （A, B and C） based on their related geuome types. The sequences of these Au element fragments were heterogouous in di-, tetra-, and hexa-ploids, and the deudrograms of Au element obtained from phylogenetic analysis were very complex in each group and could be clustered into 15, 15 and 22 families, respectively. In this study, three rules about Au elements evolution have been drawn from the results： i. Most families were composed of Au element members with different host species in three groups; ii. Family 1-6 in Group A, Family 1-6 in Group B, Family 1-4 and Family 6-13 in Group C contained only one, apparently highly degenerate Au dement member （a single representative elemeut）; iii. Elements generally fell into clades that were species-specific with respect to their host species. The potential mechanisms of Au element evolution in Aegilops were discussed.

关键词： Aegilops Au element genomic evolution sine

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出核转运抑制剂的抗肿瘤临床转化研究进展

中国肿瘤生物治疗杂志 2016年第1期23卷 106-113页

作者：刘沁颖应敏刚郑秋红福建省肿瘤医院福建医科大学教学医院福建省肿瘤生物治疗重点实验室福建福州350014

真核生物由于双层核膜的存在,蛋白及大分子物质需依赖核质转运受体进行跨核膜转运。核输出蛋白1(exportin1,XPO1)是最重要的出核转运受体之一,负责多种蛋白和RNAs的出核转运。XPO1往往在肿瘤细胞中过量表达,导致多种抑癌基因蛋白及生长... 详细信息

真核生物由于双层核膜的存在,蛋白及大分子物质需依赖核质转运受体进行跨核膜转运。核输出蛋白1(exportin1,XPO1)是最重要的出核转运受体之一,负责多种蛋白和RNAs的出核转运。XPO1往往在肿瘤细胞中过量表达,导致多种抑癌基因蛋白及生长调节蛋白亚细胞定位及功能的紊乱,从而促进了肿瘤的发生发展。XPO1不仅可作为肿瘤患者预后判断的指标,还是抗肿瘤治疗的潜在靶标。近年来,一系列XPO1小分子抑制剂的研发,尤其是高效低毒的sine系列抑制剂的抗肿瘤临床研究,揭示了XPO1抑制剂在治疗难治性、耐药性、转移复发性血液恶性肿瘤及实体肿瘤的独特疗效,并引起广泛关注。本文就出核转运抑制剂的临床转化研究进展进行综述。

关键词：核输出蛋白1 出核转运受体出核转运信号出核转运抑制剂 sine

A Logarithmic Finite Difference Method for Troesch’s Problem

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Applied Mathematics 2018年第5期9卷 550-559页

作者： M. S. Ismail K. S. Al-Basyoni Department of Mathematics College of Science King Abdulaziz University Jeddah KSA

The aim of this paper is to derive a numerical scheme for Troesch’s problem and to overcome the difficulty which faces the existing numerical methods when considering the Troesch’s problem with large values of λ. A logarithmic finite difference method is derived for solving the Troesch’s problem. The method is very simple and works well for arbitrarily large values of the Troesch’s parameter. To test the proposed method, we have used a wide range of the Troesch’s parameter λ. A comparison with some existing methods is given. The numerical results show the robustness and the superiority of the proposed scheme over most of the existing numerical methods for the Troesch’s problem.

关键词： Troesch’s Problem Logarithmic Finite Difference Method Inverse sine Hyperbolic

Flow of Generalized Burgers’ Fluid Induced by Sawtooth Pulses Stress with Quadratic Edges

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Journal of Applied Mathematics and Physics 2018年第6期6卷 1258-1277页

作者： Qamar Sultan Zaib Un Nisa Mudassar Nazar Centre for Advanced Studies in Pure and Applied Mathematics Bahauddin Zakariya University Multan Pakistan School of Mathematical Sciences University of Science and Technology of China Hefei China

This paper presents the unsteady magnetohydrodynamic (MHD) flow of a generalized Burgers’ fluid between two side walls perpendicular to a plate. The flow is generated from rest at time ?induced by stress applied on the bottom plate. The stress is assumed to be like sawtooth pulses with quadratic edges. The solutions obtained by means of the Laplace, and Fourier cosine and sine transforms are presented as a sum between the corresponding Newtonian and non-Newtonian contributions. Graphs are sketched for various parameters of interest.

关键词： Generalized Burgers’ Fluid Sawtooth Pulses Stress Fourier Cosine and sine Transforms

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水稻蜡质基因中两种类似转座因子的顺序

中国科学（B辑） 1993年第6期23卷 595-603页

作者：王宗阳郑霏琴高继平王小全吴敏张景六洪孟民中国科学院上海植物生理研究所上海200032

本实验从粳稻、籼稻与野生稻中分别克隆出了它们的蜡质基因(Wx-R-jp,Wx-R-id与Wx-R-sp),并且测定了它们的全顺序。将这3个蜡质基因的核苷酸顺序进行比较,结果表明在粳稻与籼稻蜡质基因的两个内含子与5′上游区中含有两种类似转座因子的... 详细信息

本实验从粳稻、籼稻与野生稻中分别克隆出了它们的蜡质基因(Wx-R-jp,Wx-R-id与Wx-R-sp),并且测定了它们的全顺序。将这3个蜡质基因的核苷酸顺序进行比较,结果表明在粳稻与籼稻蜡质基因的两个内含子与5′上游区中含有两种类似转座因子的结构,称之为RTL-1和RTL-2。RTL-1与缬氨酸tRNA基因及RNA多聚酶Ⅲ内部启动子顺序有一定程度的同源性。RTL-2能形成“茎环”结构。邻接RTL-1或RTL-2的两端都有短的顺向重复顺序。从分子结构上推测RTL-1类似于哺乳动物中的转座因子sine (Short Interspersed Repeated DNA Elements),而RTL-2则与果蝇中的转座因子FB(foldback)有许多相似之处。

关键词：转座因子 sine 水稻蜡质基因

The Sweetness of Chongqing Cuisine

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China Today 2020年第8期69卷 F0003-F0003页

SOUTHWEST China’s Chongqing is the city of hot *** just like the character of a person,Chongqing has another side other than what it is most famous *** the spicy thick broth of the hot pot represents Chongqing’s stubbornness and appeal,then there also hides a trace of sweetness and softness in Chongqing cuisine,which is quite rewarding when discovered.

关键词： Chongqing character sine

A Flying Spots Detection and Recovery Scheme Based on Hybrid-Waveform Recognition Method

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Energy and Power Engineering 2017年第4期9卷 63-69页

作者： Qiyue Huang Changjin Hu Zhenquan Sun Xiaoning Kang Xuze Zhang Hao Wang Chong Zhao Yali Ma Shaanxi Key Laboratory of Smart Grid (Xi’an Jiaotong University) Xi’an Jiaotong University Xi’an China Shaanxi Regional Electric Power Group CO. LTD Xi’an China Tianjin Power Transmission and Transformation Engineering Company Tianjin China

Flying plots detection has been the focus of relay protection in power system for a long time. With the promotion of Smart substation in our country, the number of SV devices is greatly increased. Abnormal data (flying plot) caused by sampling device itself has brought tremendous pressure to the power system. The traditional flying plot detection algorithm has plenty of defects, such as low pertinence, low sensitivity and long sampling period. This paper proposes a new algorithm to identify flying plot by analyzing the wave form characteristics of sampling data. The traditional waveform recognition methods are combined in this algorithm. It has the concept of standard wave window and can distinguish flying plot in a short time. In addition, sine recovery algorithm is used to recover the flying plot. This paper uses PSCAD software to verify the validity of this algorithm. Simulation results show that the proposed method has high reliability.

关键词： Hybrid-Waveform Recognition Flying Plot Abnormal Sampling sine Recovery Standard Wave Window