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simultaneous quantification of Five Stereoisomeric Hexoses in Nine Biological Matrices Using Ultrahigh Performance Liquid Chromatography with Tandem Mass Spectrometry

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Journal of Analysis and Testing 2020年第4期4卷 249-256页

作者： Jiali Zuo Runxian Cai Yanpeng An Huiru Tang State Key Laboratory of Genetic Engineering Metabonomics and Systems Biology Laboratory At Shanghai International Centre for Molecular PhenomicsZhongshan Hospital and School of Life SciencesHuman Phenome InstituteFudan UniversityShanghai 200438China

Stereoisomeric hexoses are present in almost all biological organisms in the forms of aldoses and ketoses,with diverse physi-ological and pathophysiological *** and simultaneous quantification is vital for understanding their functions ***,such analysis remains challenging owing to their highly similar behavior in chromatography and mass *** combining the pre-column 3-nitrophenylhydrazine derivatization and ultrahigh performance liquid chroma-tography tandem mass spectrometry(UHPLC-MS/MS),here,we developed a method for simultaneous quantification of five important stereoisomeric hexoses including D-glucose,D-galactose,D-mannose,D-fructose and L-sorbose representing both aldoses and *** method achieved baseline-separation for all these five derivatized hexoses chromatographically and had high sensitivity(LOD,femtomole on column),excellent linearity(R2>0.995)and efficiency with stable-isotope *** this method,we further quantified these hexoses in nine biological matrices including human biofluids(serum,urine and saliva),human cells,human and mouse feces,rat liver tissue,mung-bean seeds and peach *** results provided quantitative data for these hexoses in multiple biological samples and showed significant concentration diversity for these hexoses in different biological samples,which demonstrated the applicability of the method for simultaneous quantification of these hexose phenotypes of biological systems.

关键词： Hexose simultaneous quantification Biological matrices 3-nitrophenylhydrazine Ultrahigh performance liquid chromatography-tandem mass spectrometry

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simultaneous chromatographic fingerprinting and quantitative analysis of flavonoids in Pollen Typhae by high-performance capillary electrophoresis

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Acta Pharmaceutica Sinica B 2012年第6期2卷 602-609页

作者： Le Han Xunhong Liu Nianyun Yang Junsong Li Baochang Cai Shi Cheng Pharmaceutical College Nanjing University of Chinese MedicineNanjing 210029China

To evaluate the quality of Pollen Typhae as used in traditional Chinese medicine,a high-performance capillary electrophoresis(HPCE)method has been developed,validated and applied to chromatographic fingerprinting and quantitation of its eight main bioactive flavonoids(naringenin,isorhamnetin 3-0-(2^(G)-α-L-rhamnosy)-rutinoside,rhamnetin 3-0-neohesperidoside,isorhamnetin,quercetin 3-0-(2^(G)-α-L-rhamnosyl)-rutinoside,quercetin 3-O-neohesperidoside,kaempferol and quer-cetin).Fingerprinting was based on the selection of nine characteristic chromatographic *** quantitative analysis,the recovery of all eight compounds was in the range 98.5-102.2% with good linearity(r^(2)>0.9919)over a relatively wide concentration *** assay was successfully applied to the analysis of the eight bioactive flavonoids in 14 different *** results indicate that the assay is reproducible and precise and can be used for convenient quality assessment of Pollen Typhae.

关键词： High-performance capillary electrophoresis Pollen Typhae Fingerprint simultaneous quantification Flavonoids

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