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Asian Pacific Journal of Tropical Biomedicine 2017年第5期7卷 416-420页

作者： Kanchanat Ternchoocheep Damrassamon Surangkul Sukhgij ysothonsreekul Department of Biochemistry Faculty of Medical ScienceNaresaun UniversityMuangPhitsanulok 65000Thailand Department of Scientific Research PTT Global Chemical Public Company LimitedNoenphraMueang RayongRayong 21150Thailand

Objective:To investigate the effect of asiatic acid(AA) on the differentiated human neuroblastoma sh-sy5 y cells cytotoxic-induced by ***:Human neuroblastoma sh-sy5 y cells were either exposed to different concentrations of AA or treated with different doses of cholesterol to reveal their responding viability by MTT *** selective 1 mmol/L concentration of AA was then used to test for either the protective or the recovery effects on the cells treated with 250 mmol/L concentration of ***:AA has a propensity to directly increase the viability of differentiated human neuroblastoma sh-sy5 y *** has significant cytotoxic effect on those cells in a concentration-dependent *** has the ability to slightly recover the viability of the differentiated culture cytotoxic-induced by cholesterol but could not protect those cells from cytotoxic-induced by ***:High concentrations of cholesterol were observed to be harmful to the neurons and AA had a slight effect of reducing neuronal death caused by cholesterol.

关键词： Asiatic acid Cholesterol sh-sy5y cells Cell viability

Identification of differentially expressed proteins in sh-sy5y cells treated with resveratrol

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Neural Regeneration Research 2011年第21期6卷 1612-1617页

作者： ying Wang Zhong Dong Hongyan Fan Ming Chang Guoyi Li Linsen Hu Department of Neurology First Affiliated Hospital Jilin University Changchun 130021 Jilin Province China Department of Pharmacology Jilin Medical College of Basic Medicine Jilin 132011 Jilin Province China

To gain insight into the molecular mechanisms of resveratrol-mediated neuroprotection, two-dimensional difference gel electrophoresis in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to identify proteins differentially-expressed in sh-sy5y cells treated with resveratrol. Compared with the control group, resveratrol treatment significantly affected the expression of four proteins： endoplasmic reticulum oxidoreductin 1-like protein alpha, p21-activated kinase 1, Archain 1, and T cell receptor beta chain. The former three were downregulated and the latter was upregulated. These proteins are primarily associated with endoplasmic reticulum stress, intracellular trafficking, and immune function.

关键词： resveratrol sh-sy5y cells two-dimensional difference gel electrophoresis proteomics neural regeneration

shuyusan-containing serum protects sh-sy5y cells against corticosterone-induced impairment

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Neural Regeneration Research 2013年第22期8卷 2060-2068页

作者： Liping Chen Zhigao Sun Fawei Wang Chengyong Xu Miao Geng Hongyan Chen Dongmei Duan Department of Traditional Chinese Medicine Hainan Branch of Chinese PLA General Hospital Department of Internal Medicine Hainan Branch of Chinese PLA General Hospital Institute of Geriatrics General Hospital of Chinese PLA

The Chinese herb shuyusan, whose main constituent is jasminoidin, has been shown to protect sh-sy5y cells against corticosterone-induced damage. sh-sy5y cells injured by 400 μmol/L cor- ticosterone were treated with 5 and 30 μg/mL shuyusan-containing serum. Results revealed that shuyusan-containing serum elevated the survival rate of sh-sy5y cells, reduced Bax expression, increased Bcl-2 expression, markedly elevated brain-derived neurotrophic factor mRNA expression, and blocked cell apoptosis. Moreover, the effect of high-dose （30 μg/mL） shuyusan-containing se- rum was more remarkable. Therefore, shuyusan-containing serum appears to protect sh-sy5y cells against corticosterone-induced impairment by adjusting the expression of apoptosis-associ- ated proteins and brain-derived neurotrophic factor. Moreover, high-dose shuyusan-containing se- rum has a protective effect on high-dose corticosterone-induced impairment.

关键词： neural regeneration traditional Chinese medicine shuyusan-containing serum sh-sy5y cells corticosterone Bcl-2 Bax apoptosis brain-derived neurotrophic factor grants-supported paper neuroregeneration

Chronic neuroprotective effects of low concentration lithium on sh-sy5y cells:possible involvement of stress proteins and gene expression

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Neural Regeneration Research 2014年第7期9卷 735-740页

作者： Riadh Nciri Ezzeddine Bourogaa Samira Jbahi Mohamed Salah Allagui Abdelfattah Elfeki Christian Vincent Franoise Croute Cellular Biology Laboratory Purpan Medicine Faculty Paul Sabatier University Ecophysiology Laboratory Sciences Faculty of Sfax

To investigate the molecular mechanism underlying the neuroprotective effect of lithium on cells, in this study, we exposed sh-sy5y cells to 0.5 mmol/L lithium carbonate （Li2CO2） for 25-50 weeks and then detected the expression levels of some neurobiology related genes and post-translational modifications of stress proteins in sh-sysy cells, cDNA arrays showed that pyruvate kinase 2 （PKM2） and calmodulin 3 （CaM 3） expression levels were significantly down-regulated, phosphatase protein PP2A expression was lightly down-regulated, and casein kinase II （CK2）, threonine/tyrosine phosphatase 7 （PyST2）, and dopamine beta-hydroxylase （DBH） expression levels were significantly up-regulated. Besides, western blot analysis of stress proteins （HSP27, HSP70, GRP78 and GRP94） showed an over-expression of two proteins： a 105 kDa protein which is a hyper-phosphorylated isoform of GRP94, and a 108 kDa protein which is a phosphorylated tetramer of HSP27. These results suggest that the neuroprotective effects of lithium are likely related to gene expressions and post-translational modifications of proteins cited above.

关键词： lithium neuroprotection kinase phosphatase stress proteins sh-sy5y cells geneexpression mechanism of action

Geniposide protects human neuroblastoma sh-sy5y cells against corticosterone-induced injury

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Neural Regeneration Research 2011年第21期6卷 1618-1622页

作者： Liping Chen Fawei Wang Miao Geng Hongyan Chen Dongmei Duan Department of Traditional Chinese Medicine General Hospital of PLA Beijing 100853 China Institute of Gerontology General Hospital of PLA Beijing 100853 China

In vitro cultured human neuroblastoma sh-sy5y cells were pretreated with 50 or 5 ug/mL geniposide for 12 hours and exposed to 400 umol/L corticosterone. Corticosterone exposure in cultures not pretreated with geniposide resulted in inhibited cell growth, reduced cell survival, and increased P53 and P21 protein expression. However, in geniposide pretreated sh-sy5y cells, cell viability and the number of cells in the G2 phase of the cell cycle were significantly increased, P21 and P53 protein expression was reduced, and cell apoptosis was inhibited following corticosterone exposure. These results indicate that geniposide can protect sh-sy5y cells against high-dose corticosterone-induced injury.

关键词： Geniposide sh-sy5y cells corticosterone protein expression apoptosis

Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects sh-sy5y cells against cobalt chloride induced hypoxia

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Neural Regeneration Research 2011年第25期6卷 1947-1953页

作者： Qian yang Dianwen Gao Qingzhu Nie Zhengang Cai Jian Du Lujuan shan yuejian Liu Department of Ophthalmology Shengjing Hospital Affiliated to China Medical University Shenyang 110004 Liaoning Province China Department of General Surgery First Hospital Affiliated to Dalian Medical University Dalian 116021 Liaoning Province China Experimental Research Center First Hospital Affiliated to Dalian Medical University Dalian 116021 Liaoning Province China

In this study, we examined the effects of neuroglobin gene （Ngb） transfection into sh-sy5y cells, using ultrasound-targeted microbubble destruction （UTMD）, on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm2, a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia.

关键词： ultrasound-targeted microbubble destruction neuroglobin gene therapy recombinant plasmid sh-sy5y cells neural regeneration

Levetiracetam induces tyrosine kinase receptor B expression in sh-sy5y cells

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Neural Regeneration Research 2011年第14期6卷 1082-1085页

作者： Danrong Lei shengfu Li Xiaoyi Zou Department of Neurology West China Hospital Sichuan University Chengdu 610041 Sichuan Province China Laboratory of Transplantation Immunity Sichuan University Chengdu 610041 Sichuan Province China

Tyrosine kinase receptor B （TrkB） plays an important role in long-term potentiation and memory *** present study used all-trans retinoic acid to induce TrkB expression in sh-sy5y cells,and observed the effects of levetiracetam （LEV） on TrkB *** exposure to 10,50,and 100 μg/mL LEV,the number of TrkB-positive cells,and average absorbance value were *** demonstrated that LEV can induce TrkB expression in sh-sy5y cells.

关键词： levetiracetam tyrosine kinase receptor B brain-derived neurotrophic factor cognition sh-sy5y cells neural regeneration

Two potentially specific but relevant patterns of proteomic change Response of sh-sy5y cells to differentiation with retinoic acid followed by phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate, and susceptibility of differentiated cells to dopamine

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Neural Regeneration Research 2010年第20期5卷 1525-1533页

作者： Mingxiu Tian Xing'an Li Ming Chang yingjiu Zhang Danping Wang Hongrong Xie Linsen Hu Department of Neurology First Affiliated Hospital of Jilin University Changchun 130021 Jilin Province China Department of Neurology Fourth Municipal Hospital of Tianjin Tianjin 300140 China Key Laboratory for Honeybee Genetics and Queen Breeding Jilin Provincial Apiculture Institute Jilin 132108 Jilin Province China Key Laboratory for Molecular Enzymology and Engineering Ministry of Education Jilin University Changchun 130021 Jilin Province China

Dopamine （DA） exposure at a dose of 100 pmol/L for 24 hours causes oxidative stress in sh-sy5y cells with induction of neuronal differentiation by retinoic acid （RA,10 pmol/L,72 hours） followed by phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate （TPA,80 nmol/L,72 hours）. However,it remains unclear whether the alteration of phenotype observed in response to oxidative stress is associated with protein regulation in this cellular model for Parkinson＇s disease. The present study detected protein regulation affected by oxidative stress at a proteomic level：selection of differentially altered proteins using two dimensional difference in-gel electrophoresis and identification of these proteins using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. The results demonstrated significant alterations in expression of six proteins in sh-sy5y cells following the differentiation and fourteen proteins in the differentiated cells following the exposure,exemplified by an increase of tubulin alpha1 in the former but a decrease of tubulin alpha-ubiquitous chain in the latter. These results suggest that two potentially specific but relevant patterns of proteomic change may be produced in sh-sy5y cells with the induction of differentiation by RA followed by TPA,and in the differentiated cells after DA exposure.

关键词： sh-sy5y cells retinoic acid phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate dopamine proteomic analysis Parkinson's disease neural regeneration

Protective effects of 20-hydroxyecdysone on H_2 O_2-induced cytotoxicity in human neuroblastoma sh-sy5y cells

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Journal of Chinese Pharmaceutical Sciences 2014年第1期23卷 33-38页

作者：司霞马卓陈月黄琳冯婉玉北京大学人民医院药剂科北京100044 北京大学医学部药学院药事管理与临床药学系北京100191

The aim of this study was to investigate the possible protective effects and mechanisms of 20-hydroxyecdysone, an insect steroid hormone, on HEO2-induced cytotoxicity in human neuroblastoma sh-sysy cells. Pretreatment with 20-hydroxyecdysone significantly elevated the cell viability and decreased LDH leakage in H2O2-treated sh-sy5y cells. 20-Hydroxyecdysone also dramatically reduced malondialdehyde （MDA） contents and enhanced the superoxide dismutase （SOD） activities under oxidative stress conditions. Furthermore, 20-hydroxyecdysone pretreatment inhibited apoptosis by decreasing the Bax/Bcl-2 ratio and attenuating the activation of caspase-3. These results suggest that 20-hydroxyecdysone can protect sh-sysy cells against H2O2-induced cytotoxicity and might potentially be used to treat neurodegenerative diseases induced by oxidative stress and anontosis.

关键词： 20-Hydroxyecdysone H2O2 sh-sy5y cells Oxidative stress Apoptosis Neurodegenerative disease

Estrogen caused a calcium rise via activation of the G protein-coupled estrogen receptor in sh-sy5y cells

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Estrogen caused a calcium rise via activation of the G prote...