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CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of sars-cov-2 Delta variant

引用

Nano-Micro Letters 2022年第10期14卷 83-94页

作者： Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar AAl-Hartomy SWageh Abdullah GAl-Sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie Han Zhang International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of EducationInstitute of Microscale OptoelectronicsCollege of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060People’s Republic of China Shenzhen International Institute for Biomedical Research Shenzhen 518116People’s Republic of China Department of Respiratory Shenzhen Children’s HospitalShenzhen 518038People’s Republic of China Institute of Pediatrics Shenzhen Children’s HospitalShenzhen 518038People’s Republic of China Shenzhen Han’s Tech Limited Company Shenzhen 518000People’s Republic of China Hospital of Guangzhou Medical University Qingyuan city People’s HospitalQingyuan 511518People’s Republic of China Department of Physics Faculty of ScienceKing Abdulaziz UniversityJeddah 21589Saudi Arabia Research Center for Advanced Materials Science(RCAMS) King Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Department of Chemistry College of ScienceKing Khalid UniversityP.O.Box 9004Abha 61413Saudi Arabia Zhongmin(Shenzhen)Intelligent Ecology Co. LtdShenzhen 518055People’s Republic of China Shenzhen Center for Disease Control and Prevention Shenzhen 518055People’s Republic of China

Coronavirus disease 2019(covID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(sars-cov-2).The gold standard method for the diagnosis of sars-cov-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing ***,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of sars-cov-2 *** enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold *** DNA template identical to sars-cov-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification *** detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas ***,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing.

关键词： sars-cov-2 variant Methodology of electrochemical CRISPR sensing(MOECS) Gold nanoparticles(AuNPs) Point-of-care testing(POCT)

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