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Bioinformatics and Functional Analysis of High Oleic Acid-Related gene Gmsam22 in Soybean [Glycine max (L.) Merr.]

Phyton-International Journal of Experimental Botany 2023年第2期92卷 501-519页

作者： Shuo Qu Qi Cai Huimin Cui Lamboro Abraham Yaolei Jiao Guilong Ma Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun1300118China College of Plant Protection Agricultural UniversityChangchun1300118China

High yield,high quality,stable yield,adaptability to growth period,and modern mechanization are the basic requirements for crops in the 21st *** oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and *** as a safe fatty acid,it has the ability to successfully prevent cardiovascular and cerebrovascular *** the fatty acid composition of soybean seeds,can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans,but also have important significance in human health,and provide the possibility for the development of soybean oil as a new energy ***,the aim of this study was to analyze the high oleic acid elated gene Gmsam22 in *** this research the soybean oleic acid-related gene Gmsam22 was screened out by Genome-wide association analysis,a 662 bp fragment was acquired by specific PCR amplification,and the pMD18T cloning vector was linked by the use of a seamless cloning *** analysis of the signal peptide prediction,subcellular localization,protein hydrophobicity,transmembrane region analysis,a phosphorylation site,protein secondary and tertiary structure and protein interaction analysis of the protein encoded by the sam22 gene was carried *** plasmid of the gene editing vector is *** overexpression vector was transformed from pCAMBIA3301 as the base vector,and overexpression vector were *** plants were obtained by genetic transformation by the pollen tube channel *** quantitative PCR was performed on the T2 generation plants to detect the relative expression levels in different *** Blot was used to detect the presence of hybridization *** genes BAR,35S,and NOS in plants were identified by conventional PCR.10 seeds with high and low oleic acid content were chosen for quantitative PCR identification,and finally,the concentration and morphology of soybean fatty acids were

关键词： Soybean sam22 gene oleic acid cloning CRISPR/Cas9 expression vector

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