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s100 protein expression during induced schwann cell-like cell differentiation of rat bone marrow mesenchymal cells in vitro

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Neural Regeneration Research 2010年第3期5卷 178-184页

作者： Wenting Li Zenglu Xu Fei Ding Xiaosong Gu Institute of Basic Medical science Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100005 China Jiangsu Key Laboratory of Neuroregeneration Nantong University Nantong 226001 Jiangsu Province China

BACKGROUND： s100 protein can promote axonal growth. Therefore, transplantation of induced bone marrow-derived mesenchymal stem cells （MsCs） that can secrete s100 may provide a beneficial microenvironment for neural regeneration. OBJECTIVE： To explore the changes in s100 expression during rat MsCs differentiation into schwann ceils in vitro. DEsIGN, TIME AND sETTING： This cytology experiment was performed at the Jiangsu Key Laboratory of Neuroregeneration, Nantong University in China, from January 2006 to May 2007. MATERIALs： The rabbit anti-s100 polyclonal antibody was purchased from Dako, Denmark; the mouse anti-rat s100 monoclonal antibody was purchased from sigma, UsA. METHODs： MsCs were cultured from adult sprague-Dawley rat femur and tibia. Cell proliferation was determined by the MTT method and CD markers, and cell cycle was measured by flow cytometry. MsCs were induced to differentiate into sC cells. sC cells were stained for s100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor. s100 protein and mRNA levels were evaluated by flow cytometry, Western blot, and reverse transcription-polymerase chain reaction. MAIN OUTCOME MEAsUREs： s100 protein and mRNA expression. REsULTs： MsCs exhibited high amplification potential over eight passages. Prior to induction, the majority of MsCs were at the G0/G1 phase of the cell cycle. After induction, MsCs displayed morphology changes similar to schwann cells. Moreover, induction increased s100 mRNA levels. Immunofluorescence showed that MsCs expressed s100 protein, glial fibrillary acidic protein, and low-affinity nerve growth factor receptor at 7 days of induction. Induction also increased s100 protein levels compared with untreated MsCs. CONCLUsION： MsCs are capable of differentiating into schwann cells-like cells under conditional induction in vitro, with increasing s100 mRNA and protein expression.

关键词： bone marrow mesenchymal stem cells induction schwann cell-like cells s100 protein in vitro stem cells neural regeneration

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Immunoexpression of Cathepsin D and s100A4 protein and Their Molecular subtyptes in Canine Mammary Carcinomas

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Open Journal of Veterinary Medicine 2012年第4期2卷 163-169页

作者： Fernanda C.Figueiroa Breno s.salgado Lidianne N.Monteiro Rafael Malagoli Rocha Maria Aparecida C.Domingues Diana Martins Fernando schmitt Noeme s.Rocha Departamento de Patologia Faculdade de Medicina de BotucatuUniversidade Estadual Paulista“Julio de Mesquita Filho”BotucatuBrazil Departamento de Anatomia Patologica Hospital A.C.CamargoFundacao Antonio PrudenteSao PauloBrazil Instituto de Patologia e Imunologia Molecular da Universidade do Porto OportoPortugal Faculdade de Medicina Universidade do PortoOportoPortugal Departamento de Clínica Veterinaria Servico de Patologia VeterinariaFaculdade de Medicina Veterinaria e ZootecniaUniversidade Estadual Paulista“Julio de Mesquita Filho”BotucatuBrazil

Cathepsin D (CD), a lysosomal protease, and s100A4 protein, a calcium binding motif, are considered to be involved in metastasis in various human cancers. No data regarding such proteins are available for canine mammary carcinomas (CMCs). Accordingly, their expression in association with known factors of prognosis was investigated in this study. For that, 66 surgically resected CMCs were submitted to an immunohistochemical evaluation using anti CD, s100A4 protein, HER2, estrogen receptor α, cytokeratin 5, and p63 antibodies, further characterizing the tumors' molecular subtype. An increase in s100A4 immunoexpression by neoplastic luminal mammary cells was associated with an infiltrative tumor mode of growth, consequently leading us to conclude that s100A4 protein could be related to progression in CMCs. Additionally, the occurrence of the luminal A molecular subtype was associated with the complex histotype in CMCs. Although we have demonstrated that changes in s100A4 protein immunoexpression occurs in CMCs, further studies are needed to determine whether this represents important independent biomarkers for CMCs.

关键词： Cathepsin Mammary Tumors Metastasis-Associated proteins Molecular subtypes s100 protein

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Brazilein intervention for sciatic nerve injury in BALB/c mice

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Neural Regeneration Research 2011年第12期6卷 908-913页

作者： Zhan Zhang Jian Cao Weitian Yin Lisen Li Yuxi Jia Haoyu Liu Mingming Zhao Rihua Jiang China-Japan Union Hospital of Jilin University Changchun 130033 Jilin Province China

Brazilein has been shown to exert an immune regulation effect on regeneration in the central nervous system, but there is currently no consensus regarding its effect on sciatic nerve injury. In the current study, western blot and real-time PCR detection revealed that, after 16 and 8 g/kg of brazilein intervention, the amount of s100 protein and mRNA at L4-6 spinal segments of sciatic nerve injured mice was significantly greater than after 4 g/kg brazilein administration, and in an untreated model group. Luxol Fast Blue myelin staining revealed that neural regeneration after 16 and 8 g/kg of brazilein intervention was significantly better than after 4 g/kg brazilein administration, and in the model group. In addition, electrophysiological and immunohistochemical examinations further confirmed the effect of brazilein in repairing sciatic nerve injury in BALB/c mice. These results indicated that brazilein was able to activate the s100 in anterior horn cells of the spinal cord, promoting sciatic nerve regeneration and repair. Both moderate and high doses were found to exert significant effects.

关键词： brazilein peripheral nerve injury s100 protein sciatic nerve neural regeneration

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