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Post-self-repair process of neuron cells under the influence of neutral and cationic nanoparticles

Chinese Chemical Letters 2019年第12期30卷 2368-2374页

作者： Ting Wang Guanwen Qu Yu Deng Jing shang Zhangqi Feng Fengyu Yang Nongyue He Jie Zheng state Key Laboratory of Bioelectronics National Demonstration Centre for Experimental Biomedical Engineering EducationSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China state Key Laboratory of Natural Medicines Department of PharmacologyChina Pharmaceutical UniversityNanjing 210002China Department of Chemical and Biomolecular Engineering The University of AkronOH 44325United States school of Chemical Engineering Nanjing University of Science and TechnologyNanjing 210094China Nanjing Daniel New Mstar Technology Ltd.Nanjing 2U200 China

The prevalence of functionalized nanoparticles in biological and clinical fields attracts intensive toxicology investigations.Minimizing the nanoparticles’ biohazard remains a challenge due to the insufficient understanding on the nanoparticle-induced cell death mechanism.In the presented study,we observed the lysosome and genome injuries and so caused cell cycle changes and regulations of retinal ganglion neuron cell 5(RGC-5) induced by aminated and alkylated nanoparticles.Alkylated nanoparticles induced malignant lysosome and genome damages followed by severe post-self-repair responses.RGC-5 treated with alkylated nanoparticles presented dramatic s phase prolongation resulted from cyclin E accumulation mediated by Fbw7 downregulation,which assisted DNA replication after failed self-repair of the malignantly damaged DNA caused by alkylated nanoparticles.Differently,aminated nanoparticles in RGC-5 induced moderate lysosome and genome injuries and these damages could be repaired in the p21-involved pathway,so that cells did not induce apparent cyclin E accumulation nor Fbw7 downregulation as post-self-repair response.These results helped us to understand the toxicity of analogous nanoparticles on retinal ganglions such as glaucoma treatment.This work provides new insights into nanoparticle functionalization and toxicity in relation to the research on the toxicology and pathology of nerve cells.

关键词： Lysosome s phase Cyclin E p21 Fbw7

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study on effects of cinobufagin on the immunologic function of mice in vitro

Study on effects of cinobufagin on the immunologic function ...

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中国药理学会临床药理学专业委员会会议暨第十次全国临床药...

作者： sONG Yu~(1,2) JIANG Feng~1 WANG Da-cheng~1 ZHU Tong-fei~1 HAN Xiao-hu~1 ZHAO Wen-jie~2 DENG Xu-ming~1 1 College of Animal science and Veterinary Medicine,Jilin University,Changchun 130062,Jilin,China 2 National standard Laboratory of Pharmacology for Traditional Chinese Medicine,Jilin Natural Pharmatech Co.Ltd,Changchun 130012,Jilin,China

AIM:To investigate the immunoregulatory effects of cinobufagin from the chloroform extract of Venenum Bufo- nis,a famous traditional Chinese medicine in vitro. METHODs:Proliferation of the splenic lymphocytes in Balb/c mice was measured by MTT assay.The metaboilic level and phagotrophic activity of PMΦ were also deter- mined by MTT assay.The expressions of CD and CD and cell cycle of the splenic lymphocytes were detected by flow Cytometry. REsULTs:Administration of cinobufagin or coadminis- tration with Con A or LPs increased the cell proliferation and the percentage of s phase cells in the splenic lympho- cytes at a dose-dependent manner(P＜0.05 or P＜ 0.01).Cinobufagin also enhanced the metabolic capability of MTT and the phagocytosis function of PMΦ(P＜0.05 or P＜0.01).In addition,it was found that the single administration of cinobufagin or coadministration with Con A or LPs also could increase expressions of CD and CD in T lymphocytes(P＜0.05 or P＜0.01). CONCLUsION:single administration of cinobufagin or coadministration with Con A or LPs could enhance the re- active ability to the splenic lymphocytes and PMΦ in mice. The data indicate that the immunoregulatory effect of cinobufagin is related to the increased expression of CD and CD in DP(double positive)phenotype and s phase cells of T lymphocytes.

关键词： Venenum Bufonis Cinobufagin lymphocytes peritoneal macrophage CD4 CD8 s phase

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