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rumen liquor from slaughtered cattle as inoculum for feed evaluation

Animal Nutrition 2017年第3期3卷 300-308页

作者： Pius Lutakome Fred Kabi Francis Tibayungwa Germana H.Laswai Abiliza Kimambo Cyprian Ebong World Agroforestry Centre(ICRAF) Uganda Country Office Department of Agricultural Production School of Agricultural SciencesCollege of Agricultural and Environmental Sciences Makerere University Department of Animal Aquaculture and Range SciencesSokoine University of Agriculture Association for Strengthening Agricultural Research in East and Central Africa(ASARECA) Secretariat

Use of nonlinear mathematical models has been majorly based on in vitro gas production(GP) data generated when substrates are incubated with rumen liquor from fistulated steers. However,existing evidence suggests that rumen liquor from slaughtered cattle of unknown dietary history also generates quantifiable in vitro GP data. Fitting and description of GP data obtained from 4 diets incubated with rumen liquor from slaughtered cattle was evaluated using single-pool exponential model with discrete lag time(EXPL), logistic(LOG), Groot's(GRTS) and Gompertz(GOMP) models. Diets were formulated by varying proportions of Rhodes grass(Chloris gayana) hay and a concentrate mixed on dry matter basis to be: 1,000 g/kg Rhodes grass hay(RGH) and 0 of the concentrate(D1), 900 g/kg RGH and 100 g/kg concentrate(D2), 800 g/kg RGH and 200 g/kg concentrate(D3), 700 g/kg RGH and 300 g/kg concentrate(D4). Dietary kinetics for the models were determined by measuring GP at 2,4.8,10.18,24.36,48.72,96 and 120 h. Model comparison was based on derived GP kinetics, graphical analysis of observed versus predicted GP profiles plus residual distribution and goodness-of-fit from analysis of root mean square error(RMSE), adjusted coefficient of determination(Adj-R^2) and Akaike's information criterion(AIC). Asymptotic GP, half-life and fractional rate of GP differed(P rumen liquor from slaughtered cattle of unknown dietary history can be used to derive nutritionally important fee

关键词： In vitro gas production technique Model comparison Model selection Nonlinear mathematical models Rhodes grass hay rumen liquor

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Evaluation of the effectiveness of enzymes solubility technique in estimating digestibility of selected tropical forages

Evaluation of the effectiveness of enzymes solubility techni...

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第七届草食动物营养国际学术研讨会

作者： G.H.Laswai A.E.Kimambo G.K.Yona D.M.Mgheni T.Hvelplund M.Weisbjerg Sokoine University of Agriculture Department of Animal Science and ProductionP.O.Box 3004.MorogoroTanzania Univesity of Aarhus P.O.Box 50DK-8830.TjeleDenmark

In vitro digestibility techniques to predict in vivo digestibility of forages for ruminants have traditionally relied on the use of rumen liquor by Tilley and Terry methods. Recently the uses of artificial enzymes to replace the rumen liquor have given promising results for temperate *** is not known,however,if such methods can equally predict in vivo digestibility of tropical *** purpose of this study was to evaluate the effectiveness of enzymes solubility technique relative to rumen liquor in estimating in vivo digestibility of tropical *** enzyme solubility employed a mixture of cellulase,cellobiase,gamanase and viscozyme at a ratio of 2:1:1:1 in acetate buffer *** of maize silage(MS),Lucerne hay(LH), tropical grass mixture hay(SH)Brachiaria hay(BH),Lucerne-grass hay(LGH)and urea treated rice straw(UTRS)were milled to pass through 1 mm sieve and incubated in pepsin-HCL solution followed by the enzyme acetate buffer solution. The rumen liquor technique was modified,whereby sintered crucibles were used instead of centrifuge test *** in vitro organic matter(INOMD)digestibility values of the forages were estimated in five replicates using both *** of in vivo OM digestibility(IVOMD)of the six forages were determined using cattle at ad libitum intake of the forages as basal diet and supplemented with vitamins and *** IVOMD values(%)were 60.6,62.9,51.7,56.3,55.7 and 60.2 for MS, LH,SH,BH,LGH and UTRS,*** solubility technique tended to give higher INOMD values than the rumen liquor for some forage *** enzyme and rumen liquor techniques predicted IVOMD with low coefficients of determination(R=0.31 and 0.36,respectively).It was concluded that,although enzyme solubility technique could be used to rank digestibility values of tropical forages,more trials using large number of forage species varying in digestibility values are required to compare with in vivo data for acceptable accuracy an

关键词： digestibility enzymes rumen liquor in vivo techniques

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