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Journal of Environmental Sciences 2022年第2期34卷 129-139页

作者： Yueyao Zhou Linlin Yao Luqing Pan Hongdan Wang Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China

This study analyzed the function of different glutathione S-transferase(GST)isoforms and detoxification metabolism responses in Manila clam,ruditapes philippinarum,exposed to 4 kinds of polycyclic aromatic hydrocarbons(PAHs)single,and their mixtures for 15 days under laboratory conditions.13 kinds of GSTs in *** were classified,and the results of tissue distribution indicated that 12 kinds of GSTs(except GST sigma 3)expressed most in digestive *** detected the m RNA expression levels of aryl hydrocarbon receptor signaling pathway,and detoxification system in digestive glands of clams exposed to benzo[a]pyrene(Ba P),chrysene(CHR),benzo[a]anthracene(Ba A),benzo[b]fluoranthene(Bb F),and Ba P+CHR+Ba A+Bb F,*** these genes,we selected GST-sigma,GST-omega and GST-pi as potential indicators to Ba P;GST-sigma,GST-A and GST-rho to CHR;GST-pi,GST-sigma,GST-A,GST-rho and GST-microsomal to Ba A;GST-theta and GST-mu to Bb F;while GST-pi and GST-mu to the mixture of Ba P,CHR,Ba A and Bb ***,the bioaccumulation of PAHs in tissues increased remarkably over time,and showed an obvious *** the same concentration,the bioaccumulation in single exposure group was higher than that in mixture group,and the bioaccumulation of PAHs in tissues with different concentrations of stress was *** results revealed the metabolic differences and bioaccumulation rules in clams exposed to four kinds of PAHs,and provided more valuable information for the PAHs risk assessment.

关键词： ruditapes philippinarum PAHs bioaccumulation Glutathione S-transferase function analysis

AFLP analysis revealed differences in genetic diversity of four natural populations of Manila clam (ruditapes philippinarum) in China

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Acta Oceanologica Sinica 2007年第1期26卷 150-158页

作者： LIU Xiangquan BAO Zhenmin HU Jingjie WANG Shi ZHAN Aibin LIU Hui FANG Jianguang WANG Rucai Laboratory of Marine Genetics and Breeding （ MGB） Ocean University of China Qingdao 266003 China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Science Qingdao 266071 China

The amplified fragment length polymorphism （AFLP） technology was used to analyze the genetic diversities in four natural populations of Manila clam （ ruditapes philippinarum）, distributed in four sea areas of China, i.e. , the Bohai Sea, the Huanghai Sea, the East China Sea and the South China Sea. Two hundred and sixty-four AFLP loci were analysed in 195 individuals and revealed high levels of genetic diversity. The percentage of polymorphic loci ranged from 92.13% to 96.06% and the Shannon＇ s information index was from 0.256 8 to 0. 275 6. By analyzing molecular variance （ AMOVA）, it was found that there were high levels of genetic differentiation between populations of Qingdao and the other three sea areas. Cluster analysis by Nei＇ s pairwise distances grouped specimens by geographical origin, except the population of Qingdao. A conclusion can be drawn that there are high genetic diversities in the four natural populations of Manila clam in China and some distinct differences existed among and between the four populations. The results also indicated that human cultivation activities will have great influence on the genetic structure of the population of Qingdao.

关键词： ruditapes philippinarum genetic diversity geographical population AFLP

The influence of salinity on toxicological effects of arsenic in digestive gland of clam ruditapes philippinarum using metabolomics

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Chinese Journal of Oceanology and Limnology 2013年第2期31卷 345-352页

作者：吉成龙吴惠丰刘小莉赵建民于君宝尹秀丽 Key Laboratory of Coastal Zone Environmental Processes Yantai Institute of Coastal Zone Research(YIC)Chinese Academy of Sciences(CAS) Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes Graduate University of Chinese Academy of Sciences

ruditapes philippinarum,a clam that thrives in intertidal zones of various salinities,is a useful biomonitor to marine *** investigated the influence of dilution to 75% and 50% of normal seawater salinity(31.1) on the responses of the digestive gland of *** to arsenic exposure(20 μg/L),using nuclear magnetic resonance(NMR)-based *** acute arsenic exposure for 48 h,salinity-dependent differential metabolic responses were *** normal seawater,arsenic exposure increased the concentrations of branched-chain amino acids,and of threonine,proline,phosphocholine and adenosine,and it decreased the levels of alanine,hypotaurine,glucose,glycogen and ATP in the digestive *** changes in metabolic biomarkers observed at lower salinity(～23.3) included elevation of succinate,taurine and ATP,and depletion of branched-chain amino acids,threonine and *** effects of arsenic at the lowest salinity(～15.6) included down-regulation of glutamate,succinate and ADP,and up-regulation of *** conclude that salinity influences the metabolic responses of this clam to arsenic.

关键词： ruditapes philippinarum salinity arsenic toxicological effects nuclear magnetic resonance(NMR) metabolomics

Seawater acidification affects the immune enzyme activities of the Manila clam ruditapes philippinarum

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Journal of Oceanology and Limnology 2018年第5期36卷 1688-1696页

作者： HUO Zhongming MENG Xiangyu Md.Golam RBBANI CAO Weinan WU Qidi LI Ying WANG Jingtian YUAN Hongmei YANG Feng YAN Xiwu Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province College of Fisheries and Life Science Dalian Ocean UniversityDalian 116023China

Ocean acidification leads to changes in physiological and immune responses of bivalves, but the eff ect on the immune enzyme activities of the Manila clam, R uditapes philippinarum, when the pH is lower than the normal value has not been studied in detail. In this study, experiments were conducted to determine how pH(7.4, 7.7, 8.0) affects the immune enzyme activities in the gill and hemocytes of the Manila clam. Membrane stability and phagocytosis increased with decrease of pH from 8.0 to 7.7 and then decreased at pH 7.4. The total protein content in the hemocytes and gills decreased with decreasing pH. Lysozyme content in the hemocytes increased with decreasing pH, and the diff erences were significant among the different pH groups(P philippinarum to reduced water pH and insights for future research investigating exposure of bivalves to elevated CO_2 conditions.

关键词： seawater acidification immune enzyme ruditapes philippinarum

Annual Variations of Biogenic Element Contents of Manila Clam(ruditapes philippinarum) Bottom-Cultivated in Jiaozhou Bay, China

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Journal of Ocean University of China 2014年第4期13卷 637-646页

作者： ZAN Xiaoxiao XU Binduo ZHANG Chongliang REN Yiping College of Fisheries Ocean University of China

Manila clam （ruditapes philippinarum） was monthly sampled from its benthic aquaculture area in Jiaozhou Bay from May 2009 to June *** annual variations of major elemental composition,organic content,fatness and element ratio of Manila clam were *** element removal effect of clam farming in Jiaozhou Bay was analyzed based on natural mortality and clam *** results indicated that the variation trend of carbon content in shell （Cshell） was similar to that in clam （Cclam）.Such a variation was higher in summer and autumn than in other seasons,which ranged from 9.10±0.13 to 10.38 ± 0.09 mmol g^-1 and from 11.28 ±0.29 to 12.36±0.06 mmol g 1,*** content of flesh （Cflnesh） showed an opposite variation trend to that of shell in most months,varying from 29.42 ± 0.05 to 33.64 ± 0.62 mmol g^-*** content of shell （Nshell） and flesh （Nflesh） changed seasonally,which was relatively low in spring and *** and Nflesh varied from 0.07 ±0.009 to 0.14±0.009 mmol g^-1 and from 5.46 ± 0.12 to 7.39 ± 0.43 mmol g^-1,*** nitrogen content of clam ranged from 0.50 ± 0.003 to 0.76 ± 0.10 mmol g^-1 with a falling tend except for a high value in March *** content of clam （Nclam） fluctuated largely,while phosphorus content of shell （Pshell） was less varied than that of flesh （Pflesh）.Pshell varied from 0.006±0.001 to 0.016±0.001 mmol g^-1; while Pflesh fluctuated between 0.058±0.017 and 0.293±0.029mmolg^-*** ranged from 0.015±0.002 to 0.041 ±0.006mmolg^-*** and nitrogen content were slightly affected by shell length,width or *** contents were closely related to the reproduction *** removal amounts of carbon,nitrogen and phosphorus from clam harvest and natural death in Jiaozhou Bay were 2.92× 10^4t,1420t and 145 t,*** nutrient removal may aid to reduce the concentrations of nitrogen and phosphorus,the main causes of eutrophication,and to maintain the ecosystem health of Jiaozhou

关键词： Jiaozhou Bay Manila clam ruditapes philippinarum biogenic element ecological effect

The Effects of Ocean Acidification on Manila Clam ruditapes philippinarum

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The Effects of Ocean Acidification on Manila Clam Ruditapes ...

作者： Golam Rbbani 大连海洋大学

学位级别：硕士

The suitable culture environment is one of the determining factors of marine *** dioxide and other industrial affluent increasingly altering the environment through mixing with sea water resulting in *** poses a threat to the ecological landscape of intertidal ***,calcifying organism Manila clam ruditapes philippinarum is particularly at high risk,as increased levels of atmospheric CO2 intensify the partial pressure in the *** clam,an important commercial species cultured in Asia,need to adapt in order to cope with the adverse effects of ocean acidification or else production may decrease *** order to get a better understanding of the effect of ocean acidification,we studied the transcriptomic response of manila clam using RNA sequencing and investigate the deleterious effect as well as clam resistance to the ocean acidification.p H 8.0,7.4 and wild population of manila clam were used in this study and 8.0 p H treated as the control *** clams were cultured for 3 months in the experimental condition with sufficient food ***,total RNA was extracted from each treatment group 8.0 and 7.4 p ***,c DNA libraries were prepared using Illumina Hiseq 2000 and total 378541600 clean reads were generated after sequencing where,197912472 reads were mapped with an average mapping efficiency of 51.204%.Approximately,27,652 genes were found to be affected by *** of all unigene to GO,KEGG,SWISSPROT and nr database using BLAST for functional annotation revealed alienation of 625,67,62 and 72 genes *** the affected genes,84 genes were expressed differently（q ＜ 0.005）between treatment and control group.A further comparison showed,48 genes were Up-regulated at low p H condition and 36 genes *** annotation of the differentially expressed genes in GO and KEGG pathway reveal diverse biological functions and *** to G

关键词： ruditapes philippinarum ocean acidification

mRNA differential display on gene expression in settlement metamorphosis process of ruditapes philippinarum larvae

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High Technology Letters 2008年第3期14卷 332-336页

作者：卢素敏 Bao Zhenmin Hu Jingjie Hu Xiaoli Mu Chunhua Fang Jianguang Laboratory of Molecular Genetics Breeding Division of Marine Life Science and Technology Ocean University of China Qingdao 266003 P. R. China Key Laboratory of Chinese Ministry of Agriculture for Sustainable Utilization of Marine Fisheries Resources Yellow Sea Fisheries Research Institute Qingdao 266071 P.R. China

The mRNA differential display (DDRT-PCR) technique was adopted to find out the genes related tosettlement metamorphosis development process of ruditapes philippinarum *** this study,we haveobtained three hundred and forty-six amplification bands in total from pediveliger larvae,veliger larvae,eye spot larvae and ***-five out of three hundred and forty-six bands are distinctly differen-tial display from band pattern,which can be put into four groups,standing for different expression *** differential display bands were cloned,sequenced and analyzed and nine different se-quences are obtained in the *** sequences have higher similarity to the cDNAs deposited indatabase and three are very similar to the rDNA of other species,considered as the rDNA of *** rest three sequences are found to be novel sequences after *** accessionnumbers are AY916799,AY916798,and AY916797 *** thought the novel sequences arepossibly relevant to the early embryo development of ruditapes philippinarum larvae and can provide somefundamental understandings that are helpful for the improvement of scallop seed raising industry.

关键词： DDRT-PCR (mRNA differential display PCR) Differential gene expression Larvae development settlement metamorphosis ruditapes philippinarum

Isozyme Analysis on the Populations of ruditapes philippinarum

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Journal of Ocean University of China 2006年第1期5卷 58-62页

作者： REN Yiping GAO Tianxiang YANG Tianyan College of Fisheries Ocean University of China Qingdao 266003 P. R. China

Horizontal starch gel electrophoresis was used to investigate the genetic structure of six populations of ruditapes philippinarum in the coast of China. Seven enzymes revealed eleven putative loci, and seven of them were polymorphic, PGM^＊, MDH-1^ ＊, MDH-3^＊, LAP-1^＊, LAP-2^＊, MPI-1^＊ and MPI-2^＊. The proportions of polymorphic loci of R. philippinarum populations varied from 0.454 5 to 0.636 4. The values of observed and expected heterozygosities were from 0.039 4 to 0.154 5 and from 0.111 1 to 0.238 5, respectively. Nei＇s genetic distance varied from 0.003 3 to 0.025 3 with an average of 0.013 5. It is suggested that the genetic diversity of R. philippinarum was high.

关键词： ruditapes philippinarum isozyme genetic variation

ENDOPARASITE PERKINSNS *** THE MANILA CLAMS ruditapes philippinarum IN CHINA

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ENDOPARASITE PERKINSNS *** THE MANILA CLAMS RUDITAPES PHILIP...

AN INSITU STUDY ON BENTHIC-PELAGIC COUPLING BY BOTTOMCULTURED CLAM ruditapes philippinarum IN JIAOZHOU BAY

Ⅸ International Congress on Medical and Applied Malacology

作者： Yubo Liang Marine Environmental Monitoring Center Dalian China 116023

＜正＞Perkinsus is one of the most harmful protozoan parasites for mollusk in the *** Manila clam ruditapes philippinarum is a very commercially cultured bivalve at the coast in China,and its endoparasite Perkinsus sp had been researched following.（1） Mass morality of the clams in the coast of northern Yellow sea in China happened on July to November 2000,

关键词： ruditapes philippinarum Perkinsus sp. Epidemic Detection,China

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AN INSITU STUDY ON BENTHIC-PELAGIC COUPLING BY BOTTOMCULTURE...