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Chinese Journal of Natural Medicines 2019年第1期17卷 15-21页

作者： ZHANG Xiang-Yu LI Wei WANG Jian LI Ning CHENG Mao-Sheng KOIKE Kazuo Key Laboratory of Structure-Based Drug Design&Discovery Ministry of EducationShenyang Pharmaceutical University School of Pharmaceutical Engineering Shenyang Pharmaceutical University Faculty of Pharmaceutical Sciences Toho University

Protein tyrosine phosphatase 1 B(PTP1 B) has led to an intense interest in developing its inhibitors as anti-diabetes, anti-obesity and anti-cancer agents. The fruits of rubus chingii(Chinese raspberry) were used as a kind of dietary traditional Chinese medicine. The methanolic extract of R. chingii fruits exhibited significant PTP1 B inhibitory activity. Further bioactivity-guided fractionation resulted in the isolation of three PTP1 B inhibitory ursane-type triterpenes: ursolic acid(1), 2-oxopomolic acid(2), and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid(3). Kinetics analyses revealed that 1 was a non-competitive PTP1 B inhibitor, and 2 and 3 were mixed type PTP1 B inhibitors. Compounds 1-3 and structurally related triterpenes(4-8) were further analyzed the structure-activity relationship, and were evaluated the inhibitory selectivity against four homologous protein tyrosine phosphatases(TCPTP, VHR, SHP-1 and SHP-2). Molecular docking simulations were also carried out, and the result indicated that 1, 3-acetoxyurs-12-ene-28-oic acid(5), and pomolic acid-3β-acetate(6) bound at the allosteric site including α3, α6, and α7 helix of PTP1 B.

关键词： Protein tyrosine phosphatase 1B Raspberry rubus chingii Triterpene Ursane

Total saponins in rubus parvifolius *** lymphoma cells apoptosis through upregulated Bax/Fas and downregulated Bcl-2 in vivo and in vitro

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Traditional Medicine Research 2019年第2期4卷 99-108页

作者： Xiao-Feng Xu Ru-Bin Cheng Xue-Jin Zhang Rui-Lan Gao Department of Hematology Zhejiang Provincial Integrated Chinese and Western medicine Hospital Hangzhou China College of Pharmaceutical Science Zhejiang Chinese Medical University Hangzhou China. The First Affiliated Hospital Zhejiang Chinese Medical University Hangzhou China

Purpose:To investigate the effect of total saponins of rubus parvifolius L.(TSRP)on lymphoma Raji cells and further discuss its ***:The model of nude mice bearing Raji cells was established,the volume,weight and inhibition rate of the transplanted tumor were analyzed and compared after different concentrations of TSRP *** apoptosis and expression of Bcl-2,Bax,Fas proteins were detected by TUNEL and immunohistochemiscal method *** of TSRP on cell proliferation were tested with MTT assay in *** apoptosis and expression of Caspase-9,Caspase-3,Bcl-2,Bax and Fas proteins were tested with DAPI staining and Western ***:TSRP significantly reduced the volume and tumor weight of Raji subcutaneous transplanted tumor and induced the apoptosis of Raji cells in *** tumor inhibition rate of high-dose(100 mg/kg)TSRP is 90.84%.The TUNEL test results show that the fluorescence intensity of the tumor issue treated with TSRP is significantly *** with the control group,the fluorescence intensity of high-concentration TSRP is 82.43 ± 7.81,which is significantly different(P<0.001).The results of immunohistochemistry test showed that the Bcl-2 expression of Raji cell treated with TSRP is obviously reduced,and Bax expression is obviously ***,compared with that of control group,Fas expression is obviously *** assay showed that TSRP can significantly inhibit proliferation of Raji cells with dose *** inhibition rate of 400 μg/mL TSRP is 53.46 ± 4.90%(P<0.001).DAPI staining results showed that TSRP can significantly induce cell *** to Western blot results,it is found that TSRP can significantly inhibit activity of Bcl-2 and increase Bax expression,and TSRP can also inhibit Fas ***,expression of Caspase-9 and Caspase-3 is also ***:TSRP could inhibit the proliferation of lymphoma via induction of apoptosis in a time and dose-dependent ma

关键词： Total Saponins of rubus parvifolius L. Bcl-2 Bax Fas Apoptosis Lymphoma

Morphological Diversity of rubus pyrenes from Late Pliocene of Yunnan,SW China

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Morphological Diversity of Rubus pyrenes from Late Pliocene ...

Chemical Composition of rubus pirifolius Smith

后植物志时代的系统与进化植物学——2011年全国系统与进化植物学暨第十届青年学术研讨会

作者： Yongjiang Huang~(a,b),Zhekun Zhou~(a,c) a Key Laboratory of Biodiversity and Biogeography,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China b Graduate University of the Chinese Academy of Sciences,Beijing 100049,China c Key Laboratory of Tropical Forest Ecology,Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences,Mengla 666303,China

Fossil pyrenes are important material for investigating past biogeography and biodiversity of the genus *** pyrenes were morphologically described from Late Pliocene of Fudong Village in northwestern Yunnan Province,southwestern *** fossils are characterized by reniform,elliptic,D-shaped or drop-shaped general view,convex dorsal side,straight but sometimes curved ventral side,and reticulate surface *** show high morphological diversity and were divided into seven different morphotypes base on several *** is the first fossil record of rubus pyrenes in *** new find would significantly increase our understanding of past biogeography of this *** suggests several rubus species might have coexisted at Fudong Village during Late *** diversity of rubus might have already occurred in this region at that time.

关键词： rubus Rosoideae Sanying Formation Late Pliocene Yunnan China

来源： cnki会议评论

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Medicinal Plant 2019年第6期10卷 24-26,34页

作者： Liuping WANG Danfeng GUO Shuhui LI Zhiqi HUANG Dongping TU Jianhua WEI Guangxi University of Chinese Medicine Nanning 530200China Fangchenggang Hospital of Traditional Chinese Medicine Fangchenggang 538021China

[Objectives]This paper aimed to study the chemical composition of rubus pirifolius Smith[Methods]A preliminary experiment was carried out using the qualitative reactions of various chemical constituents,in order to roughly detect the types of chemical constituents that may be contained in *** Smith The ethanol extract of *** Smith were separated and purified using silica gel column chromatography,silica gel thin-layer chromatography and recrystallization,and the chemicals obtained were identified using mass spectrometry,nuclear magnetic resonance and literature reference.[Results]The main constituents of *** Smith are saponins,tannins,flavonoids,phytosterols,triterpenes and anthraquinones.A total of five compounds were isolated from the ethanol extract of *** Smith,and they were identified asβ-sitosterol(1),arjunolic acid(2),stigmasterol-3-O-β-D-glucopyranoside(3),euscaphic acid(4)and 3,3′,4-trimethyle llegic acid(5).[Conclusions]Compounds 1,2,3,4 and 5 were isolated from *** Smith for the first time.

关键词： rubus pirifolius Smith Ethyl acetate extract Arjunolic acid

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丽水地区悬钩子资源调查初报

浙江林业科技 1989年第2期9卷 62-63,31页

作者：麻锡亮缙云县林业局

悬钩子为第三代水果之一,有多种营养成分和医药疗效。但目前,其在我国还处于野生状态,不为人们所重视。本文就丽水地区的悬钩子资源作了初步的调查。

关键词：悬钩子属第三代水果调查初报营养成分 rubus 聚合核果小核果半常绿空心泡小裂片

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掌叶复盆子栽培性状的初步研究

植物资源与环境学报 1992年第3期8卷 40-43页

作者：赵昌民吴文龙王传永王珍霞江苏省植物研究所南京210014

经引种栽培,作者观察研究了野生掌叶复盆子(rubus chingii Hu)的栽培性状,包括植株形态、花果期、产量、果实大小等。通过对11个单株个体性状的统计分析,发现在单株产量、可育花比例、果实大小方面个体间差异极大。认为掌叶复盆子适合... 详细信息

经引种栽培,作者观察研究了野生掌叶复盆子(rubus chingii Hu)的栽培性状,包括植株形态、花果期、产量、果实大小等。通过对11个单株个体性状的统计分析,发现在单株产量、可育花比例、果实大小方面个体间差异极大。认为掌叶复盆子适合驯化栽培,野生种群中有很大的选优潜力。

关键词： rubus chingii Hu 栽培性状个体差异

Breaking Dormancy of “Tupy” Blackberry in Subtropical Conditions

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American Journal of Plant Sciences 2015年第11期6卷 1760-1767页

作者： Daniela Mota Segantini Sarita Leonel Ana Karolina da Silva Ripardo Marco Antonio Tecchio Manoel Euzebio de Souza Departamento de Horticultura Faculdade de Ciências Agron&ocircmicas Universidade Estadual Paulista (UNESP) Botucatu Brasil Departamento de Agronomia Universidade Estadual de Mato Grosso (UNEMAT) Nova Xavantina Brasil

In subtropical or tropical conditions, the insufficient winter chill accumulation is often a limiting factor to break the dormancy of temperate-climate species such as the blackberry, which requires using products to help break dormancy. This study evaluates the efficacy of compounds in breaking dormancy of blackberry and its consequent influence on phenology and crop yields. The experiment was conducted in S?o Manuel, State of S?o Paulo, Brazil, in the 2011/2012 production cycle. The plants used were two-year-old “Tupy” blackberry (rubus spp.), with 0.6 × 4.0 m spacing (4.166 plants·ha-1). Pruning was performed in August, followed by the application of these treatments: control (water);hydrogen cyanamide (Dormex?);nitrogen fertilizer (Erger?) and mineral oil (Assist?). The concentrations of each compound used were: 2.0%, 4.0%, 6.0% and 8.0%. The compounds used influenced the budding, flowering and fruit harvest stages, in addition to providing increased yields depending on the concentration used. For hydrogen cyanamide the recommended concentration is of 4.2% and 5.4% for nitrogen fertilizer;doses above these concentrations may cause phytotoxic effects. For mineral oil the dose recommended is of 8.0%.

关键词： rubus spp. Phenology Yield Growth Regulator

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树莓埋根促萌嫩梢扦插育苗技术

林业科技通讯 2017年第12期 58-60页

作者：普倩倩吕孟杰董凤祥韩德果东北农业大学园艺园林学院哈尔滨150030 中国林业科学研究院

本文介绍了树莓(rubus idaeus L.)一种新的繁殖方法,即树莓埋根促萌嫩梢扦插育苗技术,它的主要技术环节包括:材料准备、埋根、嫩梢收获、嫩梢扦插、温湿度管理、施肥及病害防治等。同目前国内树莓常规无性繁殖方法相比,该技术具有繁殖... 详细信息

本文介绍了树莓(rubus idaeus L.)一种新的繁殖方法,即树莓埋根促萌嫩梢扦插育苗技术,它的主要技术环节包括:材料准备、埋根、嫩梢收获、嫩梢扦插、温湿度管理、施肥及病害防治等。同目前国内树莓常规无性繁殖方法相比,该技术具有繁殖系数高、苗木整齐、病虫害较少、投资少、效率高等优点。

关键词：树莓 rubus idaeus 繁殖埋根促萌嫩梢扦插

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激素速蘸对黑莓嫩枝容器扦插生根的影响

林业科技通讯 2016年第9期 31-34页

作者：梁爱军山西省林业科学研究院太原030012

以黑莓(rubus fruticosus L.)嫩枝为试材,分别采用浓度为0(CK)、500、1 000mg/L的NAA、IBA、NAA+IBA处理插穗基部,并采用容器扦插的方式进行扦插生根研究。结果表明:NAA单独或NAA+IBA复合处理的生根效果较IBA单独处理的生根效果明显,其... 详细信息

以黑莓(rubus fruticosus L.)嫩枝为试材,分别采用浓度为0(CK)、500、1 000mg/L的NAA、IBA、NAA+IBA处理插穗基部,并采用容器扦插的方式进行扦插生根研究。结果表明:NAA单独或NAA+IBA复合处理的生根效果较IBA单独处理的生根效果明显,其中500mg/L NAA处理的插穗生根率达70.00%,平均生根条数达14.28,最长根长达17.40cm,根系效果指数达8.09;1 000mg/L NAA+1 000mg/L IBA复合药剂处理的插穗生根率达78.89%,平均生根条数达8.96,最长根长达19.43cm,根系效果指数达6.49,且根系生长健壮、侧根发达。因此,黑莓嫩枝扦插以1 000mg/L NAA+1 000mg/L IBA复合药剂速蘸插穗20s处理效果最佳。

关键词：黑莓 rubus fruticosus L. 激素速蘸嫩枝容器扦插

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辽西北地区树莓栽培的几项改进技术

北方果树 2017年第2期 31-32页

作者：郭长杰辽宁省果树科学研究所辽宁熊岳115009

树莓属蔷薇科（Rosaceae）悬钩子属（rubus）多年生小灌木型落叶果树,是联合国粮农组织向世界推荐的＂第三代水果＂,果实富含鞣化酸、黄酮、SOD等保健成分,具有提高人体免疫力、抗氧化防衰老等作用。树莓除可鲜食外,还可以加工成果酒、... 详细信息

树莓属蔷薇科（Rosaceae）悬钩子属（rubus）多年生小灌木型落叶果树,是联合国粮农组织向世界推荐的＂第三代水果＂,果实富含鞣化酸、黄酮、SOD等保健成分,具有提高人体免疫力、抗氧化防衰老等作用。树莓除可鲜食外,还可以加工成果酒、饮料、浓缩汁、果酱、罐头等,还被广泛用于制药、保健品、化妆品等生产领域。

关键词：悬钩子属灌木型第三代水果联合国粮农组织浓缩汁 Rosaceae 落叶果树辽西北地区秋果 rubus