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Formation of complexes of rubisco-rubisco activase from La^3+, Ce^3+ treatment spinach

Science China Chemistry 2005年第1期48卷 67-74页

作者： HONG Fashui1, LIU Chao1, ZHENG Lei1, WANG Xuefeng1, WU Kang1, SONG Weiping1, Lü Shipeng1, TAO Ye2 & ZHAO Guiwen3 1. College of Life Sciences, Suzhou Universty, Suzhou 215006, China 2. Synchrotron Radiation Laboratory, Institute of High Energy Physics, The Chinese Academy of Science, Beijing 100039, China 3. Chemical Department, University of Science and Technology of China, Hefei 230026, China College of Life Sciences Suzhou Universty Suzhou 215006 China

The complex of rubisco and rubisco activase from LaCl3-, CeCl3- treated spinach in vivo is induced. SDS-PAGE result shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only large and small subunits (55 kD, 14.4 kD) of rubisco, but also two large subunits of 45 kD and 41 kD near the large subunits of rubisco. Native-PAGE shows that the purified proteins from LaCl3-, CeCl3- treated spinach have not only a band of rubisco (560 kD), but also a band of about 1100 kD, about twice distant from rubisco, which might be a complex of rubisco and rubisco activase. The purified enzyme activities from LaCl3-, CeCl3-treated spinach are 1.8 and 2.8 times that of the control,the intensities of absorption and fluorescence are sig- nificantly higher than that of the purified rubisco from the control, and the total sulfhydryl groups and available sulfhydryl groups are 36—39 –SH per mol enzyme, 14—25 –SH per mol enzyme more than those of the purified rubisco from the control, respectively. The CD spectra show that the secondary structure of the purified enzyme from LaCl3-, CeCl3 -treated spinach is very dif- ferent from the control. The enzyme activities from LaCl3-, CeCl3- treated spinach in vivo are 1.5 and 1.9 times those of the control.

关键词： La3+, Ce3+, spinach, rubisco, complex of rubisco and rubisco acitivase, spectral characteristics.

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