检索结果-南通市图书馆

Nicotinic acetylcholine signaling is required for motor learning but not for rehabilitation from spinal cord injury

Neural Regeneration Research 2023年第2期18卷 364-367页

作者： Yue Li Edmund R.Hollis II Burke Neurological Institute White PlainsNYUSA Feil Family Brain and Mind Research Institute Weill Cornell MedicineNew YorkNYUSA Current address:Department of Neuroscience University of Rochester Medical CenterRochesterNYUSA

Therapeutic intervention for spinal cord injury is limited,with many approaches relying on strengthening the remaining substrate and driving recovery through rehabilitative *** compared with learning novel compensatory strategies,rehabilitation focuses on resto ring movements lost to *** rehabilitation of previously learned movements after spinal cord injury requires the molecular mechanisms of motor learning,or if it engages previously trained motor circuits without requiring novel learning remains an open *** this study,mice we re randomly assigned to receive intrape ritoneal injection with the pan-nicotinic,non-competitive antagonist mecamylamine and the nicotinicα7 subunit selective antagonist methyllycaconitine citrate salt or vehicle(normal saline)prior to motor learning assays,then randomly reassigned after motor learning for rehabilitation study *** rvical spinal co rd dorsal column lesion was used as a model of in complete *** of this study showed that nicotinic acetylcholine signaling was required for motor learning of the single pellet-reaching task but it was dispensable for the rehabilitation of the same task after *** findings indicate that critical diffe rences exist between the molecular mechanisms supporting compensatory motor learning strategies and the restoration of behavior lost to spinal cord injury.

关键词： acetylcholine basal forebrain corticospinal tract dorsal column lesion mecamylamine methyllycaconitine motor control rehabilitation rotarod single pellet-reaching task

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of benzene on the cerebellar structure and behavioral characteristics in rats

引用

Asian Pacific Journal of Tropical Biomedicine 2015年第7期5卷 560-565页

作者： Ali Rafati Mahboobeh Erfanizadeh Ali Noorafshan Saied Karbalay-Doust Histomorphometry and Stereology Research Centre Shiraz University of Medical Sciences Physiology Department School of Medicine Shiraz University of Medical Sciences Anatomy Department School of Medicine Shiraz University of Medical Sciences

Objective: To investigate the effects of benzene on rat's cerebellum structure and behavioral characteristics, including anxiety and motor ***: Twenty rats were randomly allocated into two groups orally receiving distilled water and benzene(200 mg/kg/day). A total of 10 rats were used at the beginning of benzene exposure. Two rats died during benzene treatment and 8 rats remained for evaluation of the behavioral test and finally 6 rats underwent histological assessment. At the end of the 4th week, motor function and anxiety were evaluated in rotarod test and elevated plus maze, respectively. Besides, the cerebellum was dissected for structural assessment using stereological ***: Performance of the benzene-treated rats in fixed and accelerating speed rotarod was impaired and their riding time(endurance) was lower compared to the control group(P = 0.02). The benzene-treated rats also spent less time in the open arms and had fewer entrances to the open arms in comparison to the control group, indicating anxiety(P = 0.01). The total volume of the cerebellar hemisphere, its cortex, intracerebellar nuclei, total number of the Purkinje, Bergmann, Golgi, granule, neurons and glial cells of the molecular layer, and neurons and glial cells of the intracerebellar nuclei were reduced by 34%-76% in the benzene-treated rats in comparison to the distilled water group(P = 0.003). The most cell loss was seen in Bergmann glia. Conclusions: The structure of cerebellum altered after benzene treatment. In addition, motor impairment and anxiety could be seen in benzene-treated rats.

关键词： Benzene Anxiety Cerebellum Stereology rotarod Elevated plus maze

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Motor and molecular analysis to detect the early symptoms in a mouse amyotrophic lateral sclerosis model

引用

Health 2013年第10期5卷 1712-1718页

作者： Valeria Valsecchi Marina Boido Antonio Piras Giada Spigolon Alessandro Vercelli Neuroscience Institute Cavalieri Ottolenghi Department of Neuroscience University of Torino Orbassano (TO) Italy

The amyotrophic lateral sclerosis (ALS) is a progressive and fatal neurodegenerative disorder primarily involving motoneurons in the cerebral cortex, brainstem, and spinal cord. SOD1-G93A mice, which express multiple copies of the mutant form of the human Cu/Zn SOD, are one of the most widely used animal models for ALS pathology. However, the onset of the disease can vary between animals of 1-2 weeks while the progression is quite fast. In order to evaluate the efficacy of any treatment, it is very important to treat all animals at the early onset of the disease, instead of at a fixed age-point. To this aim, we performed behavioral analysis and measured hSOD1 mRNA expression to identify the appearance of the first motor deficits. rotarod and PaGE tests revealed to be the most sensitive approaches to detect the beginning of the symptomatic phase of the disease, while neurological score and weight monitoring showed significant differences only at later stages in ALS pathology. Furthermore, we found a better correlation between hSOD1 mRNA expression with disease onset than with a transgene copy number. Therefore, the association of behavioral tests and molecular analysis represents a sensible and accurate tool to early detect the murine symptoms.

关键词： SOD1-G93A Mice rotarod Test PaGE Test hSOD1 Expression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

不同水平高血糖对大鼠局灶脑缺血损伤的影响

引用

中国神经精神疾病杂志 2014年第5期40卷 309-313页

作者：石见谭盛吴培华陈健南方医科大学珠江医院神经内科广州510282

目的观察不同水平高血糖对大鼠局灶脑缺血的影响。方法将23只成年雄性大鼠随机分为正常血糖组(N)6只、高血糖1组(H1)6只、高血糖2组(H2)11只。采用线栓法建立90 min局灶脑缺血模型(middle cerebral artery occlusion,MCAO),缺血开始后5 ... 详细信息

目的观察不同水平高血糖对大鼠局灶脑缺血的影响。方法将23只成年雄性大鼠随机分为正常血糖组(N)6只、高血糖1组(H1)6只、高血糖2组(H2)11只。采用线栓法建立90 min局灶脑缺血模型(middle cerebral artery occlusion,MCAO),缺血开始后5 min、50 min、95 min三个时间点,通过腹腔注射不同剂量50%葡萄糖溶液使其血糖分别维持在10 mmol/L(H1)和20 mmol/L(H2)。正常血糖组(N)在相同时间点注射生理盐水作为对照。脑缺血后24 h采用mNSS评分和rotarod评估神经损伤严重程度,TTC染色检测脑梗死面积。结果与N组相比,H2组mNSS和rotarod评分更差,脑梗死面积增加24.26%(Protarod评分以及脑梗死面积无显著差异(P>0.05)。结论卒中后严重高血糖(20 mmo/L)显著增大脑梗死面积,加重神经功能损伤,而轻度高血糖(10 mmol/L)对脑梗死面积和神经功能损伤无显著影响。

关键词：脑缺血高血糖 mNSS评分 rotarod Trc

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Roles of Sleep in the Learning and Memory of a Motor Skill in Mice

Roles of Sleep in the Learning and Memory of a Motor Skill i...

引用

中国睡眠研究会第十届全国学术年会

作者： Wan-Kun Gong Jian Ni Lu Wang Zhi-Li Huang State Key Laboratory of Medical Neurobiology Institutes of Brain Science and Collaborative Innovation Center for Brain ScienceDepartment of PharmacologySchool of Basic Medical ScienceFudan University

The question of how sleep contributes to learning and memory is currently a hot topic in the sleep field and *** role of sleep in explicit memory has been extensively studied,but whether the implicit memory will benefit from sleep remains unclear and *** dorsal striatum,the primary input nucleus of the basal ganglia,is involved in motor control and motor learning,and is poised to be a hub for *** previous works show that the dorsal striatum play an important role in sleep-wake *** striatum may be one of the targets associated with sleep and motor memory,that is,sleep may play a role in motor memory through the *** acquisition and long-term retention of motor skills are essential in our daily ***,how sleep contributes to motor skill learning and consolidation is ***,we investigated the role of sleep on motor skill in *** were trained on the accelerating rotarod task to assess the acquisition,consolidation and retrieval of motor *** were subjected to 6-h total sleep deprivation（SD） in three stages of motor skill processing through the SD ***,we used c-Fos immunohistochemical staining technique in transgenic mice to search for striatal cell ***,we used cell type specific pharmacogenetic manipulation and receptor type specific pharmacological approaches to study the mechanisms of sleep in the motor skill *** polygraphic recordings,we found that the SD device could significantly decrease both non-rapid movement（non-REM,NREM） and REM sleep during 6-h *** than 82% total sleep amount was deprived by SD devices,followed by sleep *** motor ability in mice was evaluated by open field test,pole test,stepping test and wire *** results showed that SD didn’t affect motor ability in *** the rotarod task,motor performance significantly improved in mice after 6-h SD immediately starting from training than sleeping *** c

关键词： Motor skill learning Memory consolidation Sleep deprivation Dorsal striatum rotarod

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：