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Molecular Study of rickettsiae in Serum Cattle

Open Journal of Preventive Medicine 2018年第8期8卷 260-265页

作者： Lourdes Lledó Consuelo Giménez-Pardo Rufino álamo Departamento de Biomedicina y Biotecnología Universidad Alcalá Alcalá de Henares Spain Consejería de Sanidad y Bienestar Social de la Junta de Castilla y León Valladolid Spain

A molecular survey of 230 serum samples from cattle was studied by PCR-amplification of the citrate synthase gene gltA, the gene coding for protein 190 kDa—ompA—and the gene ompB. The study was carried out in the Junta of Castilla y León (northern Spain). The results suggest that the molecular study of the serum cattle would not make a good method in epidemiological studies on rickettsiae in this region. But it is necessary to continue and expand the work with more sensitive molecular methods.

关键词： rickettsiae Epidemiology Molecular Assay

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External quality assessment of the detection of rickettsioses in China

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Asian Pacific Journal of Tropical Medicine 2010年第11期3卷 851-854页

作者： Lijuan Zhang Daxin Ni Zijian Feng Department of Rickettsiology National Institute of Communicable Disease Control and PreventionChina CDC Chinese Centers for Diseases Prevention and Control

Objective:To report a training course on the laboratory diagnoses of rickettsioses that 10 provincial/city CDCs participated in laboratory external quality assurance(EQA) based on the serological specific antibodies detection and rapid PCR amplifying targeted genes of rickettsiae. Methods:An EQA program to evaluate the following laboratory procedures was developed to detect rickettsiae:(1) immunofluorescent assay(IFA) to detect specific antibodies of A. phagocytophilum,*** and *** respectively.(2) Two sets of nested PCR were used amplifying groEL genes for most members of the family Rickettsiaceae and amplifying 16SrRNA genes for the most members of family anaplasmae,respectively.A scoring scheme based on the distribution of the median antibody titer values of the serologic assays was designed and a ranking list of the scores of the PCR results based on the detected minimal copy numbers of reference DNA was ***:Among nine laboratories who reported the results on time,eight laboratories gave acceptable serologic results,the other one provided an unacceptable antibody titer(1:2 vs median 1:64) results for *** limits of detection(LOD) for the PCR amplifying for five references DNA ranged from 1copy/μL to 10~6 copy/μ***:We successfully trained and popularized modern diagnostic methods of rickettsiae in 10 provincial CDCs in China and first conducted the EQA projects and evaluated the results.

关键词： rickettsiae Rickettsiosis EQA China

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