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Interaction between rice stripe virus Disease- Specific Protein and Host PsbP Enhances virus Symptoms

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Molecular Plant 2014年第4期7卷 691-708页

作者： Lingfang Kong Jianxiang Wu Lina Lu Yi Xu Xueping Zhou State Key Laboratory of rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310058 People's Republic of China

rice stripe virus （RSV） causes severe diseases in Oryza sativa （rice） in many Eastern Asian countries. Diseasespecific protein （SP） of RSV is a non-structural protein and its accumulation level in rice plant was shown to determine the severity of RSV symptoms. Here, we present evidence that expression of RSV SP alone in rice or Nicotiana benthamiana did not produce visible symptoms. Expression of SP in these two plants, however, enhanced RSV- or Potato virus X （PVX）- induced symptoms. Through yeast two-hybrid screening, GST pull-down, and bimolecular fluorescence complementation assays, we demonstrated that RSV SP interacted with PsbP, a 23-kDa oxygen-evolving complex protein, in both rice and N. benthamiana. Furthermore, our investigation showed that silencing of PsbP expression in both plants increased disease symptom severity and virus accumulation. Confocal microscopy using N, benthamiana protoplast showed that PsbP accu- mulated predominantly in chloroplast in wild-type N. benthamiana cells. In the presence of RSV SP, most PsbP was recruited into cytoplasm of the assayed cells. In addition, accumulation of SP during RSV infection resulted in alterations of chloroplast structure and function. Our findings shed light on the molecular mechanism underlying RSV disease symptom development.

关键词： rice stripe virus disease-specific protein interaction PsbP symptom.

Laodelphax striatellus Atg8 facilitates rice stripe virus infection in an autophagy-independent manner

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Insect Science 2021年第2期28卷 315-329页

作者： Yuan-Ling Yu Meng-Ting Zhang Yan Huo Ji-Liang Tang Qing Liu Xiao-Ying Chen Rong-Xiang Fang Li-Li Zhang State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of ScienceBeijingChina CAS Center for Excellence in Biotic Interactions University of the Chinese Academy of SciencesBeijingChina State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources Guangxi UniversityNanningChina National Plant Gene Research Center BeijingChina

rice stripe virus(RSV)is the causative agent of rice stripe disease and is completely dependent on insect vectors for its plant-to-plant *** striatellus is the major insect vector for *** this study,we explored the interactions be-tween RSV infection and *** autophagy,a potential intrinsic antiviral mechanism in *** found that *** autophagic activity did not affect RSV infection;however,the autophagy related-8(Atg8)gene significantly enhanced virus ***-ing RSV initial infection within the *** midgut,silencing of Atg8 expression significantly decreased the phosphorylation of c-Jun N-terminal kinase(p-JNK);however,when RSV infection is absent,silencing of Atg8 did not alter p-JNK *** results indicated that Atg8 might activate the JNK machinery by allowing more virus infection into *** further revealed that Atg8-deficiency significantly decreased RSV accumu-lation on the surface of the insect midgut epithelial cells,suggesting a receptor trafficking function of the y-aminobutyric acid receptor-associated protein *** the RSV ovary entry as a model,in which vitellogenin receptor(V gR)mediates RSV cell entry,we clarified that Atg8-deficiency decreased the abundance of V gR localizing on the cytomem-brane and disturbed the attachment of RSV in the germarium ***,these results revealed an autophagy-independent function of *** Atg8 that enhances RSV initial infection by increasing virus attachment on the infection sites.

关键词： Atg8 insect autophagy Laodelphax striatellus receptor trafficking rice stripe virus viral initial infection

Genetic variability and evolution of rice stripe virus

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第10期14卷 875-885页

作者： Ling-zhe HUANG Li-xia RAO Xue-ping ZHOU Jian-xiang WU State Key Laboratory of rice Biology Institute of Biotechnology Zhejiang University

rice stripe virus(RSV) is the type member of the genus *** is known to have four segmented,single-stranded RNA molecules and causes rice stripe disease in the rice fields of China,Japan,and *** on the complete genomic sequences of the determined 6 RSV isolates(from Yunnan,Jiangsu,Zhejiang,and Liaoning Provinces,China) and 27 other RSV isolates(from Yunnan,Jiangsu,Anhui,Henan,and Shandong Provinces of China,also Japan and Korea) downloaded from GenBank,we provided a genotyping profile of RSV field isolates and described the population structure of *** RSV isolates,except isolate CX,could be divided into two subtypes,one including 6 isolates from Yunnan Province,and the other including 26 isolates from different parts of China,Japan,and Korea,which were referred to as subtype II and subtype I,*** amino acid distances between subtypes range from 0.053 to *** isolates in Yunnan Province were genetically differentiated from other parts of China,Japan,and Korea and showed infrequent gene *** RSV populations collected from other parts of China,Japan,and Korea were only composed of subtype I and showed very low genetic *** speculated that isolate CX may be the result of recombination of isolates from two *** potential recombination events were detected in RNA4 of isolate CX.

关键词： rice stripe virus Genetic variability Genetic evolution

rice stripe virus NS2 Protein Interacts with SGS3 Protein of rice

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Rice Stripe Virus NS2 Protein Interacts with SGS3 Protein of...

rice St#pe virus Interferes with S-acylation of Remorin and Induces Its Autophagic Degradation to Facilitate virus Infection

中国植物病理学会第九届全国会员代表大会暨2010年学术...

作者： XIAO Dong-lai,WEI Tai-yun,WU Zu-jian~*,XIE Lian-hui (Key Laboratry of Plant Virology of Fujian Province,Fuzhou 350002,Fujian)

＜正＞Yeast two-hybrid assay was used to screen the rice cDNA library using rice stripe virus（RSV） NS2 protein as a bait.A novel rice protein interacting with RSV NS2 was obtained, which was homologous to Arabidopsis thaliana SGS3（AtSGS3） protein and was designated as *** full-length OsSGS3 gene was amplified by RT-PCR and cloned into yeast expression vector *** two-hybrid assay indicated that NS2 could interact with full-length ***3 possessed the three domains typical of AtSGS3:zinc finger,XS and coiled *** OsSGS3 deletion mutants:OsSGS3-l,which contained the znic finger domain, OsSGS3-2,which contained the XS domain,and OsSGS3-3,which contained the coiled coil domain were *** two-hybrid assay indicated that NS2 could interact with full-length OsSGS3,OsSGS3-l,OsSGS3-2 and *** localization studies indicated that OsSGS3-YFP accumulated in cytoplasmic foci that perfectly resembled those of AtSGS3 and NS2-CFP accumulated in cell wall and nuclei in Nicotiana benthamiana *** confirmed the interaction between OsSGS3 and NS2 by using co-IP,BiFC,and *** results of colocalization revealed that a considerable proportion of OsSGS3 was redistributed to nucleus by *** speculated that the redistribution of OsSGS3 by NS2 might disrupt the normal function of ***3 has been demonstrated to be involved in small RNA *** explore the possible biological implications of the interaction of RSV NS2 with OsSGS3,we first detected the expression patterns of 5 auxin responsive factors（ARFs） that had been domonstrated to be potential targets of trans acting *** results indicated that the overall accumulation levels of the 5 genes increased in RSV infected rice.

关键词： rice stripe virus NS2 yeast two hybrid Bimolecular fluorescence complementation(BiFC)

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Molecular Plant 2018年第2期11卷 269-287页

作者： Shuai Fu Yi Xu Chenyang Li Yi Li dianxiang Wu Xueping Zhou State Key Laboratory of rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310058 China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 China State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Beijing 100871 China Section of Plant Pathology and Plant-Microbe Biology School of Integrated Plant Science Cornell University Ithaca NY USA

Remorins are plant-specific membrane-associated proteins and were proposed to play crucial roles in plant-pathogen interactions. However, little is known about how pathogens counter remorin-mediated host responses. In this study, by quantitative whole-proteome analysis we found that the remorin protein （NbREM1） is downregulated early in rice stripe virus （RSV） infection. We further discovered that the turn- over of NbREM1 is regulated by S-acylation modification and its degradation is mediated mainly through the autophagy pathway. Interestingly, RSV can interfere with the S-acylation of NbREM1, which is required to negatively regulate RSV infection by restricting virus cell-to-cell trafficking. The disruption of NbREM1 S-acylation affects its targeting to the plasma membrane microdomain, and the resulting accumulation of non-targeted NbREM1 is subjected to autophagic degradation, causing downregulation of NbREMI. Moreover, we found that RSV-encoded movement protein, NSvc4, alone can interfere with NbREM1 S-acylation through binding with the C-terminal domain of NbREM1 the S-acylation of OsREM1.4, the homologous remorin of NbREM1, and thus remorin-mediated defense against RSV in rice, the original host of RSV, indicating that downregulation of the remorin protein level by interfering with its S-acylation is a common strategy adopted by RSV to overcome remorin-mediated inhibition of virus movement.

关键词： remorins S-acylation autophagy rice stripe virus movement

Dimeric artificial microRNAs mediate highly efficient RSV and RBSDV resistance in transgenic rice plants

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Dimeric artificial microRNAs mediate highly efficient RSV an...