检索结果-南通市图书馆

Food and Nutrition Sciences 2013年第9期4卷 956-963页

作者： Risa Araki Kiho Imada Makiko Hikichi Yukiko Arai Hihumi Kashima Chizuko Maruyama Department of Food and Nutrition Japan Women’s University Tokyo Japan. Department of Food and Nutrition Japan Women’s University Tokyo Japan. Email: *maruyama@fc.jwu.ac.jp

Riboflavin has been suggested to act with folate to lower homocysteine (Hcy). However, these interactions may differ among the several known forms of folate. Therefore, we examined the effects of Riboflavin interactions with 5-methyltetrahydrofolate (5-MTHF) and tetrahydrofolate (THF) on changes in Hcy and folate derivative levels, under conditions with and without methionine addition. Rat hepatocytes were cultured for 48 hours in medium with or without 2.64 μM Riboflavin addition, under the following conditions: 1) without the addition of either methionine or folate;2) with addition of 2 μM folate derivatives [(A): 5-MTHF, (B): THF];3) with addition of both 5 mM methionine and 2 μM folate derivatives [(A): 5-MTHF, (B): THF]. The supernatants were collected at 0, 24, and 48 hours for Hcy and folate derivative measurements. The Hcy lowering effect of 5-MTHF, as well as inhibition of 5-MTHF reduction and THF elevation, appeared to be enhanced by Riboflavin addition. THF addition did not lower the Hcy level, regardless of the presence of Riboflavin and/or methionine, while THF and 5,10-methenyl THF levels were maintained. Further examination is needed to elucidate the interactive effects of Riboflavin and folate derivatives on Hcy and folate metabolism.

关键词： Folate Derivatives Riboflavin Interaction Homocysteine

Protective mechanisms of Tuina therapy against lipopolysaccharide-induced fever in young rabbits based on untargeted metabolomics analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中医杂志:英文版 2023年第4期43卷 725-733页

作者： LIU Di ZHANG Yingqi YU Tianyuan LIU Zhifeng JIAO Yi WANG Hourong XU Yajing GUAN Qian CHEN Lulu HU Hui School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese MedicineBeijing 102401China Department of Acupuncture-Moxibustion Beijing Massage HospitalBeijing 100035China Department of Acupuncture-Moxibustion Dongfang HospitalBeijing University of Chinese MedicineBeijing 100078China

OBJECTIVE:To investigate the effect of Tuina on the plasma metabolites of lipopolysaccharide-induced febrile in infant rabbits.METHODS:Twenty-four infant New Zealand rabbits were selected and randomly divided into three groups:saline,model,and Tuina.The fever model was established by injecting LPS intravenously through the ear margin vein in the model group and Tuina group,respectively.The modeling was considered successful when the anal temperature increased by 0.5℃or above within 1 h.In the Tuina group,six Tuina techniques(i.e.,opening Tianmen/the heaven gate,pushing Kangong/the superciliary arch,kneading Taiyang and the prominent bone behind the ears,clearing Tianheshui,spine pinching)that alleviate fever were performed on the young rabbits 1 h after the modeling,whereas the model and saline groups were not given Tuina treatment,with the real-time anal temperature monitored during the experiment.The plasma was taken 3 h after the modeling for liquid chromatography-mass spectrometry(LC-MS)untargeted metabolomics study.RESULTS:Our results showed a fever-reducing effects of Tuina therapy on lipopolysaccharide-induced fever in young rabbits,as indicated by a significantly lower anal temperature,maximum rise in body temperature,and body response index at 2 and 3 h after modeling in the Tuina group compared to the model group,with reductions in the PGE2 expression observed in the blood and hypothalamus.The differential metabolites including Riboflavin,nicotinamide N-oxide,porphobilinogen,5-hydroxyindoleacetic acid,gamma-aminobutyric acid,and lysoPC(16:1(9Z)/0:0)were found following the Tuina intervention.Tuina primarily involves glycine-serinethreonine,arginine-proline,porphyrin-chlorophyll,pyrimidine,primary bile acid biosynthesis,and cyanoamino acid metabolic pathways.CONCLUSION:Tuina therapy has proven to be effective in reducing body temperature and down-regulating PGE2 expression in LPS-induced febrile young rabbits,with its mechanism of fever-reducing action possibly associated with the changes in plasma metab

关键词： fever pediatrics metabolomics Lipopolysaccharides Riboflavin glycine Tuina therapy

SIMULTANEOUS DETERMINATION OF VITAMIN B COMPLEX (THIAMINE, Riboflavin, NIACIN, PYRIDOXINE AND FOLIC ACID) IN FOODS BY HPLC

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1991年第8期2卷 645-648页

作者： Ya Shan HAN Xiao Qing SONG Jin Cheng ZHAO Yun Qing DAI Department of Food Science Beijing Agricultural University Beijing 100094

A high performance liquid chromatographic assay has been developed for the simultaneous determination of five water soluble vitamins:thimine, Riboflavin, niacin, pyridoxine and folic acid in cereal products and fresh vegetables. Food samples were hydrolyzed in 0.4 mol/L HCl, autoclaved at 120℃ 15 psi for 20 minutes, using a μ-Bondapak column (3.9×300mm, Waters Co.), a mobile phase of methanol-water (30:70) (0.005 mol/L heptanesulfonic acid) and a flow rate of 1.0 ml/min gave the most satisfactory separation of the five water soluble vitamins. A double channel deteetion was used: four vitamins (B_5, folic acid, B_2, B_1) were detected hy UV spectrophotometry (254 nm) first, pyridoxine (B_6) was detected by fluoromelry (EX 290nm, EM 395 nm) afterwards. Detection limits were 2, 5, 5, 2and 5ng, linear ranges were 5-10ng, 5-50ng, 5-40ng, 5-50ng and 10-50ng for B_1, B_2, B_5, B_6 and folic acid respectively. Recoveries were 92-100%(B_5), 51-52% (folio acid), 103—105% (B_2), 99—100% (B_6) and 91.3—102% (B_1) respectively. In comparison with a reference method and checking with food composition tables, very satisfactory results were obtained by this method.

关键词： PYRIDOXINE AND FOLIC ACID NIACIN Riboflavin SIMULTANEOUS DETERMINATION OF VITAMIN B COMPLEX THIAMINE IN FOODS BY HPLC

Impact of increasing one-carbon metabolites on traumatic brain injury outcome using pre-clinical models

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第8期19卷 1728-1733页

作者： Sanika M.Joshi Theresa Currier Thomas Nafisa M.Jadavji College of Osteopathic Medicine Midwestern UniversityGlendaleCAUSA Department of Biomedical Sciences Midwestern UniversityGlendaleCAUSA Department of Child Health College of Medicine–PhoenixUniversity of ArizonaPhoenixAZUSA College of Veterinary Medicine Midwestern UniversityGlendaleCAUSA Department of Neuroscience Carleton UniversityOttawaCanada

Traumatic brain injury is a major cause of death and disability worldwide,affecting over 69 million individuals yearly.One-carbon metabolism has been shown to have beneficial effects after brain damage,such as ischemic stroke.However,whether increasing one-carbon metabolite vitamins impacts traumatic brain injury outcomes in patients requires more investigation.The aim of this review is to evaluate how one-carbon metabolites impact outcomes after the onset of traumatic brain injury.PubMed,Web of Science,and Google Scholar databases were searched for studies that examined the impact of B-vitamin supplementation on traumatic brain injury outcomes.The search terms included combinations of the following words:traumatic brain injury,dietary supplementation,one-carbon metabolism,and B-vitamins.The focus of each literature search was basic science data.The year of publication in the literature searches was not limited.Our analysis of the literature has shown that dietary supplementation of B-vitamins has significantly improved the functional and behavioral recove ry of animals with traumatic brain injury compared to controls.Howeve r,this improvement is dosage-dependent and is contingent upon the onset of supplementation and whether there is a sustained or continuous delive ry of vitamin supplementation post-traumatic brain injury.The details of supplementation post-traumatic brain injury need to be further investigated.Overall,we conclude that B-vitamin supplementation improves behavioral outcomes and reduces cognitive impairment post-traumatic brain injury in animal model systems.Further investigation in a clinical setting should be stro ngly considered in co njunction with current medical treatments for traumatic brain injury-affected individuals.

关键词： folic acid nicotinamide one-carbon metabolism Riboflavin traumatic brain injury vitamin B12 vitamin B2 vitamin B3

RADIATION EFFECT ON BIOSYNTHESES OF ASCORBIC ACID AND Riboflavin IN GERMINATING CHICKPEA

在线全文

学校读者我要写书评

暂无评论

Nuclear Science and Techniques 1990年第4期1卷 222-228页

作者： Abdus Sattar S.Atta M.Wahid B.Ahmad M.A.Akhtar Nuclear Institute for Food and Agriculture PeshawarPakistan. Department of Chemistry University of PeshawarPakistan. Department of Pharmacy University of PunjabLahorePakistan

The influence of irradiation on biosyntheses of ascorbic acid and Riboflavin in germinating chickpea seeds at ambient (25-35℃) conditions, was investigated. The rate of syntheses of these vitamins significantly increased with increasing germination time upto 120 h depending upon the treatment (PRiboflavin, 11.40 and 11.38 μ g/g, on germination in tap and distilled water respectively. A significant linear relation (r = 0-954 to 0.997) was observed between the biosyntheses of these vitamins and the germination time upto 120 h of irradiated and unirradiated chickpea seed (P<0.05).

关键词： Irradiation Biosyntheses Ascorbic acid Riboflavin chickpea

PACK-CXL: Corneal cross-linking in infectious keratitis

在线全文

学校读者我要写书评

暂无评论

Eye and Vision 2016年第1期3卷 84-88页

作者： David Tabibian Cosimo Mazzotta Farhad Hafezi Laboratory for Ocular Cell Biology University of GenevaGenevaSwitzerland Siena Crosslinking Center Siena University HospitalSienaItaly Department of Ophthalmology University of Southern CaliforniaLos AngelesCAUSA EyeCare Laboratory Research Zurich Associates(ELZA)Institute Webereistrasse 28953 DietikonSwitzerland Department of Ophthalmology Northampton General HospitalNorthamptonUnited Kingdom

Background:Corneal cross-linking(CXL)using ultraviolet light-A(UV-A)and Riboflavin is a technique developed in the 1990’s to treat corneal ectatic disorders such as keratoconus.It soon became the new gold standard in multiple countries around the world to halt the progression of this disorder,with good long-term outcomes in keratometry reading and visual acuity.The original Dresden treatment protocol was also later on used to stabilize iatrogenic corneal ectasia appearing after laser-assisted in situ keratomileusis(LASIK)and photorefractive keratectomy(PRK).CXL efficiently strengthened the cornea but was also shown to kill most of the keratocytes within the corneal stroma,later on repopulated by those cells.Review:Ultraviolet-light has long been known for its microbicidal effect,and thus CXL postulated to be able to sterilize the cornea from infectious pathogens.This cytotoxic effect led to the first clinical trials using CXL to treat advanced infectious melting corneal keratitis.Patients treated with this technique showed,in the majority of cases,a stabilization of the melting process and were able to avoid emergentàchaud keratoplasty.Following those primary favorable results,CXL was used to treat beginning bacterial keratitis as a first-line treatment without any adjunctive antibiotics with positive results for most patients.In order to distinguish the use of CXL for infectious keratitis treatment from its use for corneal ectatic disorders,a new term was proposed at the 9th CXL congress in Dublin to rename its use in infections as photoactivated chromophore for infectious keratitis-corneal collagen cross-linking(PACK-CXL).Conclusion:PACK-CXL is now more frequently used to treat infections from various infectious origins.The original Dresden protocol is still used for this purpose.Careful modifications of this protocol could improve the efficiency of this technique in specific clinical situations regarding certain types of pathogens.

关键词： Infectious Keratitis Corneal cross-linking Keratoconus CXL PACK-CXL Corneal ulcer Ultraviolet light A Riboflavin

New techniques to improve classical corneal collagen cross-linking treatment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2014年第8期127卷 1558-1565页

作者： Li Na Peng Xiujun Fan Zhengjun Xia Yu Wu Tengfei Department of Ophthalmology Navy General Hospital of Chinese People's Liberation Army Beijing 100048 China

Objective The aim of this review is to comprehensively and unbiasedly summarize the improvements in the techniques for classical corneal collagen cross-linking （CXL） by covering the reasons for this improvement, measure, and effect to approach the future direction of the CXL. Data sources All articles used in this review were mainly retrieved from the PubMed database. Study selection Original articles and reviews were selected if they were related to the improvement in the technique of classical CXL. Data were mainly extracted from 94 articles, which are listed in the reference section of this review. Results This innovative research involves every step such as instrument preparation, epithelial management, Riboflavin instillation, and UVA irradiation. These clinical and experimental results seem promising. Conclusions CXL treatment is the only recent promising method for preventing the progress of keratoconus. The limitations and potential complications that accompany classical CXL such as corneal thickness limitations, ultraviolet-A （UVA） light injury, and the impact of de-epithelialization encourage people to research new improvements in techniques. While this research needs to be further investigated, we hope our review can help related researchers and patients.

关键词： cornea cross-linking keratoconus Riboflavin UVA radiation

Analysis of the effective dose of ultraviolet light in corneal cross-linking

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2016年第8期9卷 1089-1093页

作者： Yong Zhang Kuan-Chen Wang Chao-Kai Chang Jui-Teng Lin Department of Ophthalmology Shandong Provincial Hospital Shandong University Department of Electronic Engineering Taiwan University Nobel Eye Institute New Vision Inc. Taipei Taiwan 103 and Gong-Rui Medical Technology Corp.

AIM: To analyze the efficacy of ultraviolet (UV) light initiating corneal cross-linking (CXL). METHODS: The time-dependent absorption of UV light due to the depletion of the initiator (Riboflavin) was calculated. The effective dose of CXL with corneal surface covered by a thin layer of Riboflavin was derived analytically. The cross linking time was calculated by the depletion level of the Riboflavin concentration. A comprehensive method was used to derive analytic formulas. RESULTS: The effective dose of CXL was reduced by a factor (R) which was proportional to the thickness (d) and concentrations (C-0) of the Riboflavin surface layer. Our calculations showed that the conventional dose of 5.4 J/cm(2) had a reduced effective dose of 4.3 and 3.45 J/cm(2), for d was 100 and 200 pm, respectively, and C-0=0.1%. The surface cross linking time was calculated to be T*=10.75s, for a depletion level of 0.135 and UV initial intensity of 30 mW/cm(2). The volume T* was exponentially increasing and proportional to exp (bdC(0)), with b being the steady state absorption coefficient. CONCLUSION: The effective dose of CXL is reduced by a factor proportional to the thickness and concentrations of the Riboflavin surface layer. The wasted dose should be avoided by washing out the extra Riboflavin surface layer prior to the UV light exposure.

关键词： keratoconus collagen corneal cross-linking ultraviolet radiation Riboflavin safety efficacy

Changes of matrix metalloproteinases in the stroma after corneal cross-linking in rabbits

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2021年第1期14卷 26-31页

作者： Hong-Zhen Jia Xu Pang Xiu-Jun Peng Department of Ophthalmology the Sixth Medical Center of Chinese PLA General HospitalBeijing 100048China

AIM:To observe changes in the content of matrix metalloproteinases(MMPs)in the corneal stroma after corneal cross-linking(CXL)in rabbits,and further explore the corneal pathophysiological process after CXL.METHODS:Forty-two rabbits(42 eyes)were randomly divided into seven groups.One group served as the control group,while the other six groups were treated with CXL.The concentrations of MMPs in corneal stroma were evaluated through parallel reaction monitoring at baseline and 3,7,15,30,90,and 180 d after treatment.RESULTS:The levels of MMP-2 in the corneal stroma of rabbits were 0.76±0.07,2.78±1.39,4.12±0.69,2.00±0.29,2.00±0.30,1.22±0.18,and 1.35±0.18(10^(-9)mol/g)at baseline and 3,7,15,30,90,and 180 d after treatment,respectively.The contents of tissue inhibitor of metalloproteinase-1(TIMP-1)were 1.83±0.26,7.94±0.58,6.95±2.64,3.81±0.48,3.07±0.92,1.72±0.19,and 1.69±0.74(10^(-9)mol/g),respectively.The ratios of MMP-2/TIMP-1 were 0.42±0.33,0.36±0.20,0.62±0.10,0.54±0.15,0.68±0.13,0.71±0.10,and 0.68±0.09,respectively.After CXL,the expression of MMP-2 and TIMP-1 in the rabbit corneal stroma was initially increased and subsequently decreased.The levels of MMP-2 remained higher than those recorded at baseline 180 d after treatment,but it was not statistically significant.The levels of TIMP-1 returned to baseline levels at 90 d after treatment.The ratio of MMP-2/TIMP-1 started to rise from 7 d after CXL.It was significantly higher than that calculated at baseline 30-180 d after CXL.The results for MMP-1,-3,-7,-9,-13,and TIMP-2 were negative.CONCLUSION:CXL can lead to changes in the content of MMP-2 and TIMP-1 in the rabbit corneal stroma.The ratio of MMP-2/TIMP-1 remains higher versus baseline,indicating that MMP-2 is involved in the corneal pathophysiological process after CXL.

关键词： corneal cross-linking keratoconus rabbit Riboflavin matrix metalloproteinase

Liposoluble quinone promotes the reduction of hydrophobic mineral and extracellular electron transfer of Shewanella oneidensis MR-1

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Innovation 2021年第2期2卷 130-136页

作者： Xiaohan Lin Fan Yang Le-xing You Huan Wang Feng Zhao CAS Key Laboratory of Urban Pollutant Conversion Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China

A large number of reaction systems are composed of hydrophobic interfaces and microorganisms in natural environment.However,it is not clear how microorganisms adjust their breathing patterns and respond to hydrophobic interfaces.Here,Shewanella oneidensis MR-1 was used to reduce ferrihydrite of a hydrophobic surface.Through Fe(II)kinetic analysis,it was found that the reduction rate of hydrophobic ferrihydrite was 1.8 times that of hydrophilic one.The hydrophobic surface of the mineral hinders the way the electroactive microorganism uses the water-soluble electron mediator Riboflavin for indirect electron transfer and promotes MR-1 to produce more liposoluble quinones.Ubiquinone can mediate electron transfer at the hydrophobic interface.Ubiquinone-30(UQ-6)increases the reduction rate of hydrophobic ferrihydrite from 38.5±4.4 to 52.2±0.8 mM$h1.Based on the above experimental results,we propose that liposoluble electron mediator ubiquinone can act on the extracellular hydrophobic surface,proving that the metabolism of hydrophobic minerals is related to endogenous liposoluble quinones.Hydrophobic modification of minerals encourages electroactive microorganisms to adopt differentiated respiratory pathways.This finding helps in understanding the electron transfer behavior of the microbes at the hydrophobic interface and provides new ideas for the study of hydrophobic reactions that may occur in systems,such as soil and sediment.

关键词： ubiquinone wettability hydrophobic Shewanella oneidensis Riboflavin extracellular electron transfer