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Determination of genetic relationships between evergreen azalea cultivars in China using AFLP markers

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第4期14卷 299-308页

作者： Hong ZHOU Jin LIAO Yi-ping XIA Yuan-wen TENG Department of Horticulture College of Agriculture and BiotechnologyZhejiang University

Evergreen azaleas are among the most important ornamental shrubs in ***,there are probably over 300 cultivars preserved in different nurseries,but with little information available on the cultivar itself or relationships between *** fragment length polymorphism(AFLP) markers were employed to determine the genetic relationships between evergreen azalea cultivars in *** hundred and thirty genotypes collected from gardens and nurseries,including cultivars classified in the groups East,West,Hairy,and Summer,unknown cultivars,and close species,were analyzed using three primer pairs.A total of 408 polymorphic fragments were generated by AFLP reactions with an average of 136 fragments per primer *** average values of expected heterozygosity and Shannon's information index were 0.3395 and 0.5153,*** similarities were generated based on Dice coefficients,used to construct a neighbor joining tree,and bootstrapped for 100 replicates in Treecon *** coordinate analysis(PCO) was performed based on Dice distances using NTSYS-pc *** AFLP technique was useful for analyzing genetic diversity in evergreen *** analysis revealed that cultivars in the West and Summer groups were quite distinct from other groups in the four-group classification system and that the East and Hairy groups should be redefined.

关键词： rhododendron spp. Tsutsusi Fluorescent label Dice coefficient Principal coordinate analysis(PCO) Neighbor joining tree

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