检索结果-南通市图书馆

第十三次全国临床药理学学术大会

作者： Ya-Bin Sun~a,Zhong-Yuan Xu~a,Guo-Feng Li~b a GCP Office,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China b Department of Pharmacy,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China

A new,rapid and sensitive method was developed for the quantifying of Rhodamine 123(R123) in rat plasma using liquid chromatography tandem mass spectrometry(LC-MS/MS).rhodamine 6G(R6G) was used as the internal standard(IS).R123 and IS were extracted from aliquots of plasma with ethyl acetate and dichloromethane (4:1) as the solvent.Chromatographic separation was performed using a Zorbax Eclipse Plus C18 column.The mobile phase was composed of A:ammonium formate-formic acid buffer containing 5 mM ammonium formate and 0.1%formic acid and B:methanol(A:B,5:95,v/v).To quantify R123 and IS respectively,multiple reaction monitoring(MRM) transition of m/z 345.2→285.2 and m/z 443.3→415.2 were performed.The analysis lasted for 4 min,in the positive mode,the results of which were presented as a linear calibration curve over the concentration range of 1-200 ng/ml and sampling volume of 2μl.The lowest limit of quantification(LLOQ) reached 1 ng/ml.The intra and inter-day precision were 6.7%and 9.2%for the low quality control(QC) sample (2 ng/ml),respectively.Intra and inter-day precision of the medium QC sample(100 ng/ml) were both less than 3.4%.Intra and inter-day precision of high QC sample(150 ng/ml) were both less than 2.1%,while the intra and inter-day relative errors ranged between -7.4%and 9.1%for the above three QC concentration levels.The LC-MS/MS method proved to be simple,accurate,reliable and with a shorter running time and has been successfully applied to an absorption experiment in the rat.

关键词： Rhodamine 123 Liquid chromatography tandem mass spectrometry rhodamine 6G Rat plasma

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Rhodamine 123(Rh-123)对癌细胞与正常细胞的作用比较及机制分析

引用

解剖学报 1988年第4期 448-448页

作者：钟杰章静波中国医学科学院基础医学研究所

Rh-123是一种活细胞线粒体的荧光探针。在黑暗的背景下可显示清晰的线粒体荧光图像。在一定的浓度下,它对正常细胞无明显毒性作用,所以是研究线粒体形态与功能的有利工具。近年来又发现Rh-123具有选择性地杀伤肿瘤细胞的作用。本实验选... 详细信息

Rh-123是一种活细胞线粒体的荧光探针。在黑暗的背景下可显示清晰的线粒体荧光图像。在一定的浓度下,它对正常细胞无明显毒性作用,所以是研究线粒体形态与功能的有利工具。近年来又发现Rh-123具有选择性地杀伤肿瘤细胞的作用。本实验选用人子宫颈癌HeLa细胞及正常人胚肾成纤维细胞为材料,用多种方法比较了Rh-123对它们的作用,并探讨其作用机制。

关键词： Rhodamine 123 正常细胞线粒体粒线体

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fluorometric Viability Assessment of Capacitated and Acrosome-Reacted Boar Spermatozoa by Flow Cytometry

引用

Optics and Photonics Journal 2013年第1期3卷 40-44页

作者： Reyna Fierro Humberto Gonzalez-Marquez Rocio Ortiz Jerome Chevrier Bernard Foliguet Departamento de Ciencias de la Salud Universidad Autonoma Metropolitana-IztapalapaMexico CityMexico Laboratoire d’Histologie Embryologie et Microscopie ElectroniqueUniversite LorraineNancyFrance

Sperm capacitation involves functional changes, such as the removal or appearance of specific molecules and changes in the plasma membrane;the acrosome reaction (AR) is an exocytotic event induced by calcium influx, enabling the spermatozoa to penetrate the zona pellucida. These processes can be achieved only if the spermatozoa have good viability;indeed, determination of sperm viability is used for the assessment of semen quality. Membrane integrity and mitochondrial activity are important viability parameters of spermatozoa and fluorescent techniques based on membrane permeability to dyes have been developed to determine these parameters. The aim of this work was to determine the viability of boar sperm (fresh, one hour of capacitation induction and 20 min of AR induction) by flow cytometry using propidium iodide (PI) (1.25 μg/mL) and Rhodamine 123 (R123) (0.20 μg/mL). Aliquots of 5 × 105 sperm were incubated with each fluorochrome separately and simultaneously for 10 or 20 min, respectively, at 38℃. The proportion of labeled spermatozoa and their fluorescence intensities were measured using a flow cytometer. The fluorescence index (FI) with PI gradually increased during the incubation and we found significant differences between all the groups. With R123, the FI increased in the capacitated sperm but decreased in the acrosome-reacted sperm, with significant differences between the fresh and capacitated spermatozoa. Our results suggest that the increase in the R123 fluorescence intensity in capacitated spermatozoa is due to changes in the mitochondrial membrane activity because the spermatozoa experienced changes in membrane fluidity and flagellar activation during capacitation. The use of fluorochromes and flow cytometry is a good tool for monitoring many markers of sperm function. Although capacitation and AR processes have been well studied, there is still much information to be elucidated with regard to these complex processes.

关键词： Boar Sperm Flow Cytometry Propidium Iodide Rhodamine 123 Viability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：