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Gene Expression Patterns in Different Wool Densities of rex rabbit Using cDNA Microarray

Agricultural Sciences in China 2011年第4期10卷 595-601页

作者： CHEN Sai-juan LIU Tao LIU Ya-juan DONG Bing GU Zi-lin College of Animal Science and Technology Agricultural University ofHebei Baoding 071001 P.R. China Ringpu （Baoding） Biological Pharmaceutical Co. Ltd. Baoding 071000 P.R. China Mountain Area Research Institute Agricultural University of Hebei Baoding 071001 P.R.China Division of Scientific Research Agricultural University of Hebei Baoding 071001 P.R.China

This study was carried out to identify gene expression patterns in different wool densities of rex rabbit. The mid-dorsal skin samples from 8 rex rabbits were studied. They were divided into two groups according to different wool densities. Total RNA was isolated and labeled by reverse transcription reaction with Cy5-dCTP and Cy3-dCTP for cDNA probe. The cDNA probe was hybridized with cDNA microarrays containing 14 601 rabbit’s genes. The differently expressed genes were analysed with the Gene Ontology （GO） classification and the pathway analysis. Hierarchical clustering was performed to clarify genes in association with different wool densities. The 2 657 differentially expressed genes were identified. Among them, 1 103 genes were functionally known genes, 687 genes were up-regulated and 419 were down-regulated. GO analysis indicated that these altered genes were associated with metabolism, signal transduction, cell cycle, cell adhesion, cell proliferation, cell division, apoptosis, and other processes. KEGG analysis showed that 95 signal pathways associated with up-regulated genes and 87 signal pathways associated with down-regulated genes had changed significantly （P0.05）. Some important differentially expressed genes in different wool densities of rex rabbit were identified, such as MMP2, TGF-β1, TGF-β2, IGF-1, ITGB1, RPS6KB1, BMP2, ActRIIB, CDK2, and CCNA2. Hierarchical clustering analysis separated the differentially expressed genes into 5 main characteristic groups. Data from cDNA microarray experiments clearly distinguished between group A and group B. Some important genes were identified, which might be useful in further study on wool density markers of rex rabbit.

关键词： rex rabbit wool density gene expression cDNA microarray GO and KEGG analysis

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Phenotypic and Genetic Characteristics of a Bordetella hinzii Isolated from a rex rabbit

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Animal Husbandry and Feed Science 2015年第3期7卷 163-166页

作者： Li Shuguang Shen Zhiqiang Qu Guanggang Guan Yu Fu Shijun Hu Linlin Wang Yumao Shandong Binzhou Animal Science and Veterinary Medicine Academy Shandong Lvdu Bio-Science and Technology Co. Ltd.

A Bordetella hinzii strain, isolated from the lung of a dead breast-feeding infant rex rabbit with a respiratory infection, was used as study object to investigate its phenotypic and genetic characteristics. The strain could grow on MacConkey agar and was gram-negative, oxidase-positive, catalase-positive, short rodshaped, and it can produce alkali from malonate. Its 6S rRNA sequence was partially identical with B. hinzii （AF177667）. Animal experiments revealed nasal mucous hyperemia, tracheal congestion and bronchopneumonia in 28-day old rex rabbits. To sum up, this Bordetella hinzii isolate was deemed as a novel causative agent of respiratory disease in rex rabbit.

关键词： Bordetella hinzii rex rabbit 16S rRNA sequencing Animal experiments

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Differential Expression of CCNA2 Gene and MMP2 Gene in Skin Tissue of rex rabbit with Different Fur Densities

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Animal Husbandry and Feed Science 2016年第5期8卷 257-260页

作者： Chen Saijuan Liu Yajuan Chen Baojiang Liu Tao Gu Zilin Mountain Research Institute Hebei Agricultural University Baoding 071001 China Mountain Area of Hebei Agricultural Engineering Technology Research Center Baoding 071001 China College of Animal Science and Technology Hebei Agricultural University Baoding 071001 China Ringpu (Baoding) Biological Pharmaceutical Co. Ltd. Baoding 071000 China

[ Objective] The paper was to search for the molecular marker highly associated with fur density of rex rabbit. [ Method ] Experimental rex rabbits were divided into dense fur group and sparse fur group. The variation rule of gene expression in skin tissue of rex rabbits with different fur densities was detected using rabbit whole gene cDNA microarray. [ Result] A total of 2 657 genes with differential expression were detected by microarray test, including 1 106 genes with known function, 687 genes with up-regulated expression and 419 genes with down-regulated expression. The expression levels of multiple genes involving in cell proliferation, differentiation, apoptosis and migration had significant changes. MMP2 gene had high expression in skin tissue of rex rabbits in dense fur group, which was 8. 195 times of gene expression in sparse fur group ; CCNA2 gene had low expression in skin tissue of rex rabbit in dense fur group, which was 0. 143 times of gene expression in spaarse fur group. [ Conclusion ] The results provide the theoretical basis and research direction for early breeding of rex rabbit in practical production.

关键词： rex rabbit Fur density Gene expression Microarray

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Thickness Method for Measuring Hair Density of rex rabbit

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Animal Husbandry and Feed Science 2018年第2期10卷 81-83,108页

作者： Gao Haiqi Li Bingjing Liu Han Zhang Zongcai Key Laboratory of Leather Chemistry and Engineering of the Ministry of Education Sichuan University Chengdu 610065 China National Engineering Laboratory for Clean Technology of Leather Manufacture Sichuan University Chengdu 610065 China

[Objective] The paper was to characterize hair density by changes in hair thickness under certain pressure.[Method] Tile effects of weight of thickness tester and wafer urea at the hottom of presser foot on measurement results were studied, and the optimal measuring weight and wafer diameter was determined. The measurement results of hair thickness under the ahove condition were compared with the results of manual counting. Meanwhile, the effects of hair length and hair fineness on hair density were analyzed.[Result] The hair density obtained by manual count- ing had consistent change trend with the hair thickness measured under certain conditions. If the length and fineness of hairs were introduced for modification, the correlation between hair thickness and hair density would be better. There were little changes in hair length and finelless of rex rabbit, and their effects on hair thickness could be ignored under general condition. [Conclusion] It is completely feasible to characterize hair den- sity of rex rabbit by hair thickness under pressure.

关键词： rex rabbit Hair density Measurement method Thickness

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Effects of Different Feed Additives on Fur Production Performance of rex rabbit

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Animal Husbandry and Feed Science 2010年第4期2卷 34-35页

作者： SUN Quan-wen Hebei North University Zhangjiakou 075029 China

[ Objectivel To investigate the effects of three different levels of feed additives on fur production performance of rex rabbit. [ Method] A total of 60 healthy American strain rex rabbits at the age of 60 d （female：male =1：1 ） were randomly divided into group I, group II and group III. The rex rabbits in the group I were fed basal diet containing nutritional substances; those in the group II were fed basal diet containing nutdtionai substances and Chinese herbal additive; and those in the group III were fed basal diet containing nutritional substances, Chinese herbal additive and enzyme. The trial lasted for 100 d. After slaughtering, the fur area, fur thickness, wool density, wool fineness and coarse wool rate were determined. [Result] The fur production performance of group III was significantly higher than that of group I （ P 〈 0.05）. There was no significant difference between group III and group II. [ Conclusion] Supplementing Chinese herbal additive and enzyme in diet can improve the fur production performance of rex rabbit.

关键词： rex rabbit Feed additive Fur production performance

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Coprophagous behavior of rex rabbit affects growth and female gut microbiome

Coprophagous behavior of rex rabbit affects growth and femal...

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中国畜牧兽医学会动物微生态学分会第十一次全国学术研讨会暨第五届会员代表大会

作者： Shushu Han Ping Wang Bo Zeng Hanzhong Liu Wensu Jian Kai Zhang Ning Liu Fanli Kong Jiangchao Zhao Ying Li Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University Sichuan Academy of Grassland Science Department of Pediatrics and Communicable Disease University of Michigan

Coprophagy plays an important role in nutrition and ingestion for small- and medium-sized herbivorous *** different nutrient components between soft feces and hard feces and the adverse effect of preventing coprophagy on growth had been ***,*** et *** that the behavior of rabbit pups ingesting doe’s hard feces affected the implantation of cecal bacterial ***,the effect of coprophagous behavior of re-ingesting soft feces of oneself on microbiome is still *** hypothesized that preventing re-ingesting soft feces could change the gut microbiota composition and this change will affect the growth of the host *** test our hypothesis,we chose 111 healthy weaning rex rabbits,which were randomly assigned to three groups:in group 1(Prevent),rex rabbits wore homemade collars around necks to prevent coprophagy;in group 2(AUow),rex rabbits wore shorted homemade collars as control to group 1,which allow rex rabbits to access to soft feces;in group 3(Control),rex rabbits wore no collars,which as blank *** sampled the hard feces and weighted the rabbits at day 40,49,56,63,70,84,98,*** samples of 11 rex rabbits per group at different time points were used to assess the dynamics of the bacterial *** V4 region of 16 S rRNA genes was sequenced using Illumina MeSiq *** the sequences were analyzed using QIIME *** found that the weight of rex rabbits in three groups had no significant difference at the start point of the ***,we observed that the preventing coprophagy group grew significantly slower than the two control groups of ***,the allow group and the control group had similar microbiota composition at phylum and genus ***,Female individuals from prevent group had higher levels of Bacteroides and ClostridiumIVa and lower proportions of Ruminococcaceae and Clostridiales than Allow *** group and male individuals in

关键词： rex rabbit coprophagy gut microbiome growth

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Effect of Chinese Herbal Feed Additive on Meat Quality of rex rabbit

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Agricultural Science & Technology 2009年第2期10卷 122-126页

作者：袁政委蒲万霞陈国顺刘渤涛高海霞中国农业科学院兰州畜牧与兽药研究所甘肃兰州730050 甘肃农业大学动物科学技术学院甘肃兰州730070 甘肃省饲料工程中心甘肃兰州730020

[ Objective] The aim of this study was to provide a theoretical basis for population and application of Chinese herbal feed additive in the rex rabbit breeding. [ Methyl] 108 weaned -rex rabbits aged 40 days were divided into three groups with thirty -six each group. Group A were fed basal diet, while group B were fed basal diet supplemented with 0.3% Chinese herbal feed additive and group C were fed basal diet supplemented with 0.4% Chinese herbal feed additive. The meat quality indexes were measured when feeding to 150 age in days. [ Result] There was significant difference among pH value, water loss rate, storing loss rate and crude protein content in each group （P〈0.05）, but no significant difference was found in tenderness, cooking percentage, moisture content and crude fat content （ P 〉 0.05）. The total content of ami- no acids, essential amino acids and semi essential amino acids as well as main delicious amino acids was highest in group C accounting for 23.84%, 11.47% and 8.32% respectively, next came group B accounting for 20.94%, 10.64% and 6.81% respectively, and lowest in group C （ control group） accounting for 19.06%, 9.61% and 6.46% respectively. [ Conclusion] The Chinese herbal feed additive can improve meat quality of rex rabbit the best addition of 0.4%.

关键词： Chinese herbal feed additive rex rabbit Meat quality

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Pyridoxine regulates hair follicle development via the PI3K/Akt, Wnt and Notch signalling pathways in rex rabbits

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Animal Nutrition 2021年第4期7卷 1162-1172页

作者： Gongyan Liu Guangmin Cheng Yongcui Zhang Shuxia Gao Haitao Sun Liya Bai Shu Li Yanli Zhu Chunyang Wang Fuchang Li College of Animal Science and Technology Shandong Agricultural UniversityShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and PreventionTai'an 271018China Institute of Animal Husbandry and Veterinary Shandong Academy of Agricultural SciencesShandong Key Laboratory of Animal Disease Control and BreedingJinan 251000China Shandong Vocational Animal Science and Veterinary College Weifang 261061China

This study was conducted to evaluate the effect of pyridoxine on the development of hair follicles in rex rabbits and the underlying molecular *** hundred 3-month-old rex rabbits were randomly divided into 5 groups and fed diets supplemented with 0,5,10,20,or 40 mg/kg *** hair follicle density on the dorsal skin and the gene and protein expression levels of components of the phosphoinositide 3-kinase(PI3 K)/protein kinase B(PKB or Akt),Wnt,Notch and bone morphogenetic protein(BMP)signalling pathways were *** addition,free hair follicles were isolated from rex rabbits and cultured with pyridoxine in vitro to measure hair shaft ***,dermal papilla cells(DPC)were isolated from the skin of rex rabbits and cultured with pyridoxine in vitro to measure the gene and protein expression levels of components of the PI3 K/Akt,Wnt,Notch and BMP signalling *** results showed that the addition of dietary pyridoxine significantly increased the total follicle density,secondary follicle density,and secondary-to-primary ratio(S/P,Prex rabbits(Prex rabbits probably occurs through activation of the PI3 K/Akt,Wnt and Notch signa

关键词： Hair follicle development Pyridoxine rex rabbit Dermal papilla cell

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