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第三届全国植物开花·衰老与采后生物学大会

作者： Ying Miao Fujian Provincial Key Laboratory of Plant Functional Biology College of Life Sciences Fujian Agriculture and Forestry University

Plant senescence are the final stages of plant development, which are controlled by a series of specific gene expressions in the development process, as well as by internal and external environmental factors, accompanied by nutrition and energy remobilization. It has known that plant hormones, calcium, metabolites and redox system in cells are involved in the regulation of cell senescence. These internal signals are produced from chloroplast and mitochondria, and the external environmental signals, such as light, temperature, and even bacterial stress, are directly responded by chloroplast and mitochondrion, connecting the signal between organelle and nucleus. Previously our group identified a kind of dual-located proteins, such as WHIRLY family proteins, which are located in both organelle and nucleus, and make various functions and communication in/between two cell compartments. Therefore, WHIRLY protein would be the alternative regulatory factors of retrograde signal pathway. Based on the establishment of an experimental system for the response and detection of environmental signals, we attempt to establish a model of chloroplast and mitochondria working as sensors of environmental signals to regulate plant senescence and programmed cell death via retrograde signalling of plastid-nucleus.

关键词： WHIRLY proteins retrograde signalling plant senescence plastid-nucleus

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Phosphorylation of WHIRLY1 by CIPK14 Shifts Its Localization and Dual Functions in Arabidopsis

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Molecular Plant 2017年第5期10卷 749-763页

作者： Yujun Ren Yanyun Li Youqiao Jiang Binghua Wu Ying Miao Center for Molecular Cell and Systems Biology Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology College of Life Sciences Fujian Agriculture and Forestry University Fuzhou 350002 China These authors contributed equally to this article

Plastid-to-nucleus retrograde signaling is critical for normal growth and development in plants. The dualfunction and dual-located ssDNA binding protein WHIRLY1 （WHY1） has been proposed to coordinate the retrograde signaling from plastids to the nucleus. However, the regulatory mechanism governing the functional switch of WHY1 for mediating plastid-to-nucleus retrograde signaling remains unknown. Here, we report that the Calcineurin B-Like-Interacting Protein Kinase14 （CIPK14） interacts with and phosphorylates WHY1 in Arabidopsis. Phosphorylation of WHY1 results in increased accumulation in the nucleus and enhanced binding with the promoter of WRKY53, which encodes a key transcription factor regulating leaf senescence in Arabidopsis. Transgenic plants overexpressing CIPK14 showed an increased nuclear isoform but decreased plastid isoform of WHY1, among which 95% of transgenic lines showed the stay-green phenotype and 5% of lines showed the variegated pale-green phenotype. Interestingly, the phenotypes of both types of transgenic plants could be recovered by overexpression of plastid-form WHY1. In contrast, knockdown of ClPK14 caused early senescence and even seedling-lethal phenotypes along with elevated expression of senescence-related genes such as WRKY53, SAG12, and NDHF but decreased expression of MER11, RAD50, and POR genes, which could be rescued by overexpression of CIPK14 but not by overexpressing plastid-form or nuclear-form WHY1; the stay-green plants overexpressing ClPK14 showed reduced expression of WRKY53, SAG12, NDHF, and large plastid rRNA. Consistently, the accu- mulation of nuclear-form WHY1 was significantly reduced in the CIPK14 knockdown lines, resulting in a low ratio of nuclear-/plastid-form WHY1. Taken together, our results demonstrate that CIPK14 regu- lates the phosphorylation and organeUar distributions of WHY1 and pinpoint that ClPK14 may function as a cellular switch between leaf senescence and plastid development for coordinating the int

关键词： WHIRLY1 CIPK14 retrograde signalling Leaf Senescence Plastid Development

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