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Journal of Integrative Agriculture 2024年第11期23卷 3843-3859页

作者： Youli Wang Huajin Zhou Jing Chen Yuqin Wu Yuming Guo Bo Wang Jianmin Yuan Key Laboratory of Qinghai Tibetan Plateau Animal Genetic Resource Reservation and Utilization Ministry of Education/Key Laboratory of Sichuan Province for Qinghai Tibetan Plateau Animal Genetic Resource Reservation and ExploitationCollege of Animal Science and Veterinary MedicineSouthwest Minzu UniversityChengdu 610041China State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Sichuan New Hope Liuhe Technology Innovation Co.Ltd. Chengdu 610100China

Fasting is typically used before feeding metabolizable energy assessment in *** studies have shown that fasting cause atrophy of the intestinal *** fasting affects intestinal permeability during refeeding by altering barrier function and nutrient absorption is of ***,23-d-old broilers were randomly assigned to 5 treatments,fasted for 0,12,24,36,and 48 h,respectively,and then refed for 2 d,to study the impact of different duration of fasting on the intestinal regeneration and barrier function during *** showed that the intestinal morphology in fasted birds was recovered in 2 d of refeeding at *** fasting durations increased,enterocytes per intestinal villus were linearly and quadratically increased(both Pretinol metabolism when compared to the nonfasted birds(Pretinol benefits the cell proliferation and enterocyte *** conclusion,the intestinal permeability to small and large molecule

关键词： broiler chicken fasting intestinal barrier intestinal permeability retinol

retinol dehydrogenase 10 promotes metastasis of glioma cells via the transforming growth factor-β/SMAD signaling pathway

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Chinese Medical Journal 2019年第20期132卷 2430-2437页

作者： Feng Guan Zhuang Kang Liang Wang Ke Wang Bei-Bei Mao Wei-Cheng Peng Bo-Lun Zhang Zhen-Yang Lin Jun-Ting Zhang Zhi-Qiang Hu Department of Neurosurgery Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Neurosurgery Beijing Tiantan HospitalCapital Medical UniversityBeijing 100050China

Background:Glioma is the most common primary malignant tumor in the central nervous system. Because of the resistance of glioma to chemoradiotherapy and its aggressive growth, the survival rate of patients with glioma has not improved. This study aimed to disclose the effect of retinol dehydrogenase 10 (RDH10) on the migration and invasion of glioma cells, and to explore the potential ***:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the expression levels of RDH10 in healthy glial cells and glioma cells. Human glioma cell strains, U87 and U251, were infected with negative control or RDH10-interfering lentiviruses. RT-PCR and Western blotting were performed to determine the knockdown efficiency. Scratch and transwell assays were used to assess cell migration and invasion after RDH10 knockdown. Finally, changes in transforming growth factor-β (TGF-β)/SMAD signaling pathway-related expression were examined by Western blotting. Differences between groups were analyzed by one-way analysis of ***:RDH10 was highly expressed in glioma cells. Compared with the control group, RDH10 knockdown significantly reduced RDH10 messenger RNA and protein expression levels in U87 and U251 glioma cells (U87: 1.00 ± 0.08 vs. 0.22 ± 0.02, t= 16.55, P < 0.001;U251: 1.00 ± 0.17 vs. 0.39 ± 0.01, t= 6.30, P < 0.001). The scratch assay indicated that compared with the control group, RDH10 knockdown significantly inhibited the migration of glioma cells (U87: 1.00% ± 0.04% vs. 2.00% ± 0.25%, t= 6.08, P < 0.01;U251: 1.00% ± 0.11% vs. 2.48% ± 0.31%, t= 5.79, P < 0.01). Furthermore, RDH10 knockdown significantly inhibited the invasive capacity of glioma cells (U87: 97.30 ± 7.01 vs. 13.70 ± 0.58, t = 20.36, P < 0.001;U251: 96.20 ± 7.10 vs. 18.30 ± 2.08, t = 18.51, P < 0.001). Finally, Western blotting demonstrated that compared with the control group, downregulation of RDH10 significantly inhibited TGF-β expression, phosphorylated SMAD2, and

关键词： retinol dehydrogenase Metastasis Glioma RNA Lentivirus

retinol促进小鼠雄性生殖干细胞的增殖

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中国兽医学报 2013年第2期33卷 307-311,316页

作者：褚志礼孙军伟刘超王龙胡玥华进联西北农林科技大学动物医学院陕西省干细胞工程技术研究中心农业部动物生物技术重点实验室陕西杨凌712100

retinol(RE)作为维生素A的一种,对生命有机体内多种生理生化和代谢反应均有重要的影响。本试验以雄性生殖干细胞(mGSCs)为对象,用RE培养mGSCs,探讨RE对mGSCs增殖的影响。试验分别设置对照组,RE组,RE+LY(LY294002,PI3K抑制剂)组,RE+PD(PD... 详细信息

retinol(RE)作为维生素A的一种,对生命有机体内多种生理生化和代谢反应均有重要的影响。本试验以雄性生殖干细胞(mGSCs)为对象,用RE培养mGSCs,探讨RE对mGSCs增殖的影响。试验分别设置对照组,RE组,RE+LY(LY294002,PI3K抑制剂)组,RE+PD(PD98059,MAPKK抑制剂)组。选取培养不同时间的mGSCs进行半定量与定量PCR分析,检测基因的表达情况。对培养48h的mGSCs进行Brdu荧光染色。结果显示,RE培养能够显著促进mGSCs的增殖,培养后C-myc等增殖标记基因表达上调,加入LY、PD抑制剂后,增殖效果显著降低;表明RE可能通过PI3K或MAPKK促进mGSCs的增殖。本研究证明了RE具有促进mGSCs增殖的作用,对于探明RE对mGSCs增殖调控的机理具有重要意义。

关键词： retinol 雄性生殖干细胞增殖小鼠

A Placebo-Controlled Study Demonstrates the Long-Lasting Anti-Aging Benefits of a Cream Containing retinol, DihydroxyMethylChromone (DMC) and Hyaluronic Acid

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Journal of Cosmetics, Dermatological Sciences and Applications 2012年第2期2卷 51-59页

作者： Thierry Oddos Romain Roure James Leyden Valérie Bruère Christiane Bertin Johnson & Johnson Santé Beauté France Issy-les-Moulineaux France Johnson & Johnson Santé Beauté France Val-de-Reuil France KGL Inc. Broomall USA

retinol is an ingredient used in cosmetic products for reducing the appearance of the signs of aging and photo-damage. Currently, most of these products contain 0.1% of retinol. However, at this concentration, some irritation can occur. We have evaluated in vitro and in a clinical study the potential efficacy of a combination of actives to improve the facial skin aging signs while using low concentration of retinol. We demonstrated, in vitro, that a chromone derivative, 5,7-di-hydroxy-2-methyl chromone (DMC), is able to enhance the collagen synthesis in culture of normal human dermal fibroblasts. The enhancement of retinol anti-wrinkle efficacy by DMC was confirmed in a small scale clinical trial. Specifically, a product associating low concentration of retinol (0.04%) and DMC (0.1%) in combination with low molecular weight hyaluronic acid fragments (50,000 Dalton of average molecular weight) has been applied topically for 8 weeks. Clinical results show significant improvement of various signs of facial skin aging such as wrinkles, pigmentary spots, tone unevenness, dullness and the overall photo-damage score. Improvements were still visible 4 weeks after the cessation of the test product application. This study demonstrates that significant lasting improvement of facial skin aging can be obtained with well tolerated low concentration of retinol when adequately formulated with other anti-aging ingredients.

关键词： retinol DihydroxyMethylChromone Hyaluronic Acid Anti-Age Long-Lasting Effect

Safe retinol-Like Skin Biological Effect by a New Complex, Enriched with retinol Precursors

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Journal of Cosmetics, Dermatological Sciences and Applications 2020年第2期10卷 59-75页

作者： Ze’evi Ma’or Dror Cohen Ya’ara La’or-Costa Meital Portugal-Cohen Ahava Dead Sea Laboratories Lod Israel The Skin Research Institute the Dead-Sea and Arava Science Center Masada Israel

Background: retinol (RE) is deeply involved in skin processes, therefore it is widely formulated in cosmetics, primarily as an anti-aging ingredient. Despite its efficacy, the safety profile of RE is controversial. Objectives: Pretinol (PRE) complex was formulated with two RE precursors, β-Carotene and Niacinamide, in order to deliver retinol-like skin benefits with healthier characteristics, assuming that skin enzymes will enable safe RE supply on spot. Methods: The expression levels of hyaluronic acid, Tumor Necrosis Factor alpha (TNFα) and Interleukin 1 alpha (IL-1α), were measured using various skin models before and after exposure to PRE and RE. Full genome microarray was performed and the affected genes and pathways were analyzed. Results: Following fibroblasts exposure to PRE, the natural synthesis of hyaluronic acid is significantly elevated. Skin safety, demonstrated via cytokines expression on ex-vivo skin, results with TNFα and IL-1α elevation by RE application. In contrary PRE significantly reduces TNFα while IL-1α is not affected. These results establish skin safety advantage of PRE vs RE. Microarray results examined on skin equivalents reveal the involvement of PRE in inflammatory attenuation. Conclusions: Formulating RE precursors as a safe source for RE is partially supported. PRE presents a skin benefit in parallel to RE, while PRE characteristics are suggested to be safer to skin.

关键词： retinol Hyaluronic Acid β-Carotene Skin Inflammaging Irritation

Reviscometer Evaluation of the Skin Condition after Applying retinol and Vitamin C Complex in Menopausal Women

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Journal of Cosmetics, Dermatological Sciences and Applications 2016年第4期6卷 148-155页

作者： Aneta Wójcik Ewelina Bartnicka Helena Rotsztejn Department of Cosmetology and Aesthetic Dermatology Faculty of Pharmacy Medical University of Lodz Lodz Poland Department of Cosmetic Raw Materials Chemistry Faculty of Pharmacy Medical University of Lodz Lodz Poland

Background: Topical application of retinol connected with the improvement of various parameters of aging skin is often used in post- and menopausal women. Vitamin A - vitamin C complex treatments may influence skin surface lipids and boost the fibroblasts activity as well as increase their number, thus inducing the improvement of its elasticity. Objective: In the current paper, we demonstrate and evaluate the impact of retinol and vitamin C complex on the face and neck skin elasticity level in a group of women aged 50 - 69. Method: Reviscometer RVM 600 (Courage & Khazaka, Germany) was used to measure forehead, cheeks and neck skin elasticity in a group of 21 women. Each of the patients was subjected to four treatment sessions that included skin elasticity measurements before each of the three retinol peels at 3-week intervals and one 3-week after the completion of the therapy. Results: Significant increase in the cheeks and neck skin elasticity was observed in the study group. Conclusion: Treatments including retinol with vitamin C - vitamin A complex significantly influence the improvement of skin elasticity, thus reducing the signs of skin aging.

关键词： retinol Vitamin C - Vitamin A Complex Reviscometer

Effects of Hydrocortisone, Glycerophosphate and retinol on the Differentiation of Mesenchymal Stem Cells and Vascular Endothelial Cells to Osteoblasts

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Journal of Biomedical Science and Engineering 2014年第13期7卷 1056-1066页

作者： Naofumi Shiomi Keiko Watanabe School of Human Sciences Kobe College Nishinomiya Japan

Vascular calcification, which causes occlusion and rupture of the vascular, is often observed in patients in the advanced stages of arteriosclerosis. One of the best procedures for inhibiting the accumulation of vascular calcification is to obstruct the differentiation of mesenchymal stem cells (MSCs) and/or vascular endothelial cells (VECs) in the vascular to osteoblasts. In this study, we evaluated the biochemical and genetic characteristics of the process of differentiation of MSCs and VECs to osteoblasts. C3H10T1/2 MSCs, TKD2 VECs and MC3T3-E1 preosteoblasts (POBs) were cultured in medium containing both hydrocortisone and glycerophosphate. These compounds showed strong effects promoting the differentiation of VECs as well as POBs, although the effect was weak in the MSCs. Moreover, C3H10T1/2 MSCs and TKD2 VECs were cultured in medium containing 10 mM retinol, after which the alkali phosphatase (ALP) activity of the MSCs and production of calcified nodules of TKD2 were significantly increased, whereas the marker genes for the osteoblasts were not. These results suggest that retinol does not have an effect in inducing the differentiation of VECs to osteoblasts, but rather exhibits a strong promoting effect on differentiation.

关键词： Vascular Calcification Osteoblasts Vascular Endothelial Cells Mesenchymal Stem Cells retinol Hydrocortisone

Characterization of chitosan/α-lactalbumin nanocomplex particle and its encapsulation for retinol

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FOOD BIOSCIENCE 2023年第1期51卷 397-404页

作者： Jiang, Zhanmei Li, Jinzhe Chen, Wei Hussain, Muhammad Altaf Wei, Xuan Bilawal, Akhunzada Hou, Juncai Northeast Agr Univ Coll Food Sci Key Lab Dairy Sci Minist Educ Harbin 150030 Peoples R China Lasbela Univ Agr Water & Marine Sci Uthal 90150 Balochistan Pakistan Northeast Agr Univ Key Lab Dairy Sci Minist Educ Harbin 150030 Peoples R China

Chitosan (Ct) and alpha-lactalbumin (ALa) were applied as the carriers, their biopolymer nanoparticle was designed to encapsulate retinol (VA). Zeta potential and turbidity measurements, demonstrated that the most suitable pH value in the formation of Ct/ALa nanoparticle was 6.0, and its optimal Ct/ALa mass ratio was 1:4. At this ratio, scanning electron microscopy (SEM) of Ct/ALa nanoparticle showed that Ct was coated on the surface of ALa/VA as a shell. Meanwhile, the encapsulation efficiency (EE) and loading capacity (LC) of above nano-sized Ct/ALa particle for VA were 82.22 +/- 1.78% and 3.13 +/- 0.12%, respectively. Moreover, Ct/ALa nanoparticle not only improved the storage stability of VA, but also protected VA from the destruction of simulated gastrointestinal juice to a certain extent during in vitro digestion. Information from this study could supply the novel opinion for the protection and delivery of photosensitive bioactive substances (VA) in functional dairy-based product.

关键词： retinol ?-Lactalbumin Chitosan Nanoparticle Stability Digestion

Rdh13 deficiency weakens carbon tetrachloride-induced liver injury by regulating Spotl4 and Cyp2e1 expression levels

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Frontiers of Medicine 2019年第1期13卷 104-111页

作者： Xiaofang Cui Benting Ma Yan Wang Yan Chen Chunling Shen Ying Kuang Jian Fei Lungen Lu Zhugang Wang State Key Laboratory of Medical Genomics Research Center for Experimental MedicineRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai Research Center for Model Organisms Shanghai 201203China Department of Gastroenterology Shanghai First People s HospitalShanghai Jiao Tong UniversityShanghai 200080China

Mitochondrion-localized retinol dehydrogenase 13 (Rdh13) is a short-chain dehydrogenase/reductase involved in vitamin A metabolism in both humans and mice. We previously generated Rdh13 knockout mice and showed that Rdh13 deficiency causes severe acute retinal light damage. In this study, considering that Rdh13 is highly expressed in mouse liver, we further evaluated the potential effect of Rdh13 on liver injury induced by carbon tetrachloride (CC14). Although Rdh13 deficiency showed no significant effect on liver histology and physiological functions under regular culture, the Rdh13^-/- mice displayed an attenuated response to CCl4-induced liver injury. Their livers also exhibited less histological changes and contained lower levels of liver-related metabolism enzymes compared with the livers of wild-type (WT) mice. Furthermore, the Rdhl3 1 mice had Rdh13 deficiency and thus their liver cells were protected from apoptosis, and the quantity of their proliferative cells became lower than that in WT after CC14 exposure. The ablation of Rdhl3 gene decreased the expression levels of thyroid hormone-inducible nuclear protein 14 (Spot14) and cytochrome P450 (Cyp2el) in the liver, especially after CC14 treatment for 48 h. These data suggested that the alleviated liver damage induced by CC14 in Rdh13^-/- mice was caused by Cyp2el enzymes, which promoted reductive CC14 metabolism by altering the status of thyroxine metabolism. This result further implicated Rdhl3 as a potential drug target in preventing chemically induced liver injury.

关键词： retinol dehydrogenase 13 carbon tetrachloride acute liver injury Cyp2el Spot14

Study on the interaction between β-carotene and gut microflora using an in vitro fermentation model

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Food Science and Human Wellness 2023年第4期12卷 1369-1378页

作者： Zhixian Li Zhuqing Dai Enjuan Shi Peng Wan Guijie Chen Zhongyuan Zhang Yayuan Xu Ruichang Gao Xiaoxiong Zeng Dajing Li School of Food and Biological Engineering Jiangsu UniversityZhenjiang 212013China Institute of Agro-product Processing Jiangsu Academy of Agricultural SciencesNanjing 210014China College of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China

β-Carotene,a typical non-oxygenated carotenoid,is the most efficient source of retinol(VA).The low bio-availability ofβ-carotene lead to large accumulation in colon;however,the relationship betweenβ-carotene and gut microflora remains *** study intends to explore the interaction betweenβ-carotene and gut microflora using an in vitro fermentation *** 24 h fermentation,the degradation rate ofβ-carotene was(64.28±6.23)%,which was 1.46 times that of the group without gut ***,the production of VA was nearly 2 times that of the group without gut microflora,indicating that the gut microflora can metabolizeβ-carotene into VA.β-Carotene also influences the production of short-chain fatty acids(SCFAs),the production of total SCFAs in 0.5 mg/mLβ-carotene(BCM)group was(44.00±1.16)mmol/L,which was 2.26 times that of the blank control(BLK)*** them,the production of acetic acid in BCM group was(19.06±0.82)mmol/L,which was 2.64 time that of the BLK ***,β-carotene significantly affected the structure and composition of gut microflora,increasing the abundance of Roseburia,Parasutterella and Lachnospiraceae,and decreasing the abundance of Dialister,Collinsella and Enterobacter(P<0.05).This study provides a new way to understand howβ-carotene works in human body with gut microflora.

关键词： β-Carotene Gut microflora retinol Short-chain fatty acids