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The peptide fraction of Bothrops jararaca snake venom induces toxicological effects on the male reproductive system after local envenomation in mice

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BIOCELL 2023年第2期47卷 289-295页

作者： CARLOS ALBERTO-SILVA ANA CAROLINA DE ARAUJO RODRIGO SIMÃO BONFIM JOYCE MEIRE GILIO Natural and Humanities Sciences Center Experimental Morphophysiology LaboratoryFederal University of ABC(UFABC)São Bernardo do Campo09606-070Brazil Departamento de Biofísica Universidade Federal de São PauloSão Paulo04023-062Brazil

Bothrops envenomation is complex and provokes prominent local tissue damage and systemic disturbances,but little is known about their effects on the male reproductive system.After intratesticular injection,the bioactive peptide fraction(Bj-PF)obtained from Bothrops jararaca snake venom changes the structure of different stages of the seminiferous epithelium cycle in adult mice.For the first time,we investigated whether local envenomation of Bj-PF induces toxicological effects on the male reproductive system,particularly on the seminiferous epithelium and Sertoli cells.Male adult mice were treated with 0.24 mg.kg^(-1) by intramuscular(i.m.)injection for 24 h.The testes samples were collected for morphological and morphometric evaluation.The toxicological effects of Bj-PF were also analyzed on mitochondrial metabolism and nitrite(NO2)production in 15P-1 Sertoli cell culture.Bj-PF changed the structure and function of the seminiferous epithelium,particularly the disruption of the epithelium and the presence of degenerated germ cells in the adluminal compartment,but there were no alterations in the basal compartment.Bj-PF increased the thickness of the seminiferous epithelium and decreased the lumen diameter of the tubule.Semiquantitative histological assessment of the degree of tubule degeneration revealed that Bj-PF also increased the number of hypospermatogenic tubules compared to control.Bj-PF reduced NO2 levels in 15P-1 Sertoli cells without changing the mitochondrial metabolism.Overall,the fact that Bj-PF alters the structure and function of the seminiferous epithelium suggests that bioactive peptides found in B.jararaca snake venom can have toxicological effects on the reproductive systems of affected male mice,providing new insight into the biological characteristics of snake venom and therapeutic strategies for envenomation inflammation.

关键词： Snake venom Bothrops jararaca reproductive toxicology Seminiferous epithelium

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Assessing human sperm morphology： top models, underdogs or biometrics？

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Asian Journal of Andrology 2010年第1期12卷 36-46页

作者： Jacques Auger Service d＇Histologie-Embryologie Biologie de la Reproduction/CECOS Hopital Cochin Paris 75014 France

The assessment of the percentage of spermatozoa having an ＇ideal＇ morphology using so-called strict method is the method recommended in the latest edition of the World Health Organization （WHO） laboratory manual for semen analysis. This recommendation is a result of the statistical association between ＇ideal＇ sperm morphology and fertility, and of the current general belief that sperm morphology assessment should be used primarily as a fertility tool. The notion of an ＇ideal＇ sperm morphology has persisted despite the very low percentage of such spermatozoa in the semen of fertile men, a subject of intense controversy. The detailed categorization of each abnormal spermatozoon has thus, for a long time, been considered optional and partially redundant, an idea which is reflected in the earlier editions of the WHO manual. However, several recent studies have shown the importance of carefully assessing abnormal sperm morphology for use in the diagnosis ＆infertility, to determine fertility prognosis, and for basic or public health studies. One approach, which combines videomicroscopy and computer vision, and is the only approach able to assess the continuum of sperm biometrics, has been used successfully in several recent clinical, basic and toxicology studies. In summary, the visual assessment of detailed sperm morphology--including the categorization of anomalies allowing arithmetically derived indices of teratozoospermia--and the more modern computer-based approaches, although often considered to be redundant, are in fact complementary. The choice of the most appropriate method depends on the field of investigation （clinical, research, toxicology） and the problem being addressed. Each approach has advantages as well as certain limitations, which will be discussed briefly herein.

关键词： diagnosis fertility prognosis image analysis multiple anomalies index pattern recognition reproductive toxicology spermdefects sperm deformity index sperm pathology teratozoospermic index videomicroscopy

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Analysis of the role of long non-coding RNA involved in atrazine-induced testicular degeneration of Xenopus laevis

Analysis of the role of long non-coding RNA involved in atra...

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中国毒理学会第七次全国会员代表大会暨中国毒理学会第六次...

作者： Linlin Sai Qiang Jia Cunxiang Bo Yu Zhang Hua Shao Cheng Peng Shandong Academy of Occupational Health and Occupational Medicine The University of Queensland Queensland Alliance for Environmental Health Sciences(QAEHS)

Some long non-coding RNAs （lncRNAs） participate in epigenetic regulation which plays a critical role in male germline development by binding chromatin modifying protein complexes and regulating protein-coding gene expression. Atrazine （AZ）, an environmental endocrine disrupting chemical （EDCs） that can induce male reproduction toxicity of amphibians.Data from our previous studies demonstrated that chronic exposure to AZ can alter gene and circular RNAs （circRNAs） expression of damaged testes in developing Xenopus laevis （X. laevis）.We furthered the study to investigate the lncRNA expression profiling in the testis of developing X.laevis chronically exposed to 100μg/L AZ for which predicted analysis including Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway and competing endogenous RNAs analysis were performed. Over 4652 lncRNAs were detected by lncRNA sequencing of testis tissues from control groups （n=3） and AZ-treated X. laevis （n=3）. Treatment of AZ led to 95lncRNAs （45 upregulated and 50 downregulated） differentially expressed compared with froglets in the control groups. The reproducibility and reliability of RNA-seq were confirmed by Q-RTPCR. The results of KEGG pathway analysis showed that AZ-affected lncRNAs mainly involved in 19 pathways among which 12 pathways are also in the results of circRNA analysis. This study for the fisrt time provides evidence that AZ can alter lncRNAs which may play a role in AZinduced testicular degeneration through regulating expressions of functional genes in the testes of X. laevis. Our data may provide more insights on the mechanism about male reproductive toxicity of EDCs.

关键词： herbicide lncRNA ceRNA frog reproductive toxicology

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