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remote ischemic preconditioning protects liver ischemia-reperfusion injury by regulating eNOS-NO pathway and liver microRNA expressions in fatty liver rats

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Hepatobiliary & Pancreatic Diseases International 2017年第4期16卷 387-394页

作者： Yun-Fei Duan Yong An Feng Zhu Yong Jiang Department of Hepatobiliary Surgery The Third Affiliated Hospital of Soochow UniversityChangzhou 213003China

BACKGROUND: ischemic preconditioning (IPC) is a strategy to reduce ischemia-reperfusion (I/R) injury. The protective effect of remote ischemic preconditioning (RIPC) on liver I/R injury is not clear. This study aimed to investigate the roles of RIPC in liver I/R in fatty liver rats and the involvement of endothelial nitric oxide synthase-nitric oxide (eNOS-NO) pathway and microRNA expressions in this process. METHODS: A total of 32 fatty rats were randomly divided into the sham group, I/R group, RIPC group and RIPC+I/R group. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and nitric oxide (NO) were measured. Hematoxylin-eosin staining was used to observe histological changes of liver tissues, TUNEL to detect hepatocyte apoptosis, and immunohistochemistry assay to detect heat shock protein 70 (HSP70) expression. Western blotting was used to detect liver inducible NOS (iNOS) and eNOS protein levels and realtime quantitative polymerase chain reaction to detect miR-34a, miR-122 and miR-27b expressions. RESULTS: Compared with the sham and RIPC groups, serum ALT, AST and iNOS in liver tissue were significantly higher in other two groups, while serum NO and eNOS in liver tissue were lower, and varying degrees of edema, degeneration and inflammatory cell infiltration were found. Cell apoptosis number was slightly lower in the RIPC+I/R group than that in I/R group. Compared with the sham group, HSP70 expressions were significantly increased in other three groups (all P<0.05). Compared with the sham and RIPC groups, elevated miR-34a expressions were found in I/R and RIPC+I/R groups (P<0.05). MiR-122 and miR-27b were found significantly decreased in I/R and RIPC+I/R groups compared with the sham and RIPC groups (all P<0.05). CONCLUSION: RIPC can reduce fatty liver I/R injury by affecting the eNOS-NO pathway and liver microRNA expressions.

关键词： fatty liver ischemia-reperfusion remote ischemic preconditioning nitric oxide heat shock protein 70 endothelial nitric oxide synthase inducible nitric oxide synthase liver microRNA

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Protective effects of remote ischemic preconditioning in rat hindlimb on ischemia- reperfusion injury

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Neural Regeneration Research 2012年第8期7卷 583-587页

作者： Ying Zhang Xiangrong Liu Feng Yan Lianqiu Min Xunming Ji Yumin Luo Cerebrovascular Diseases Research Institute Xuanwu HospitalCapital Medical UniversityMinistry of EducationBeijing 100053China Department of Neurology Xuanwu Hospital of Capital Medical UniversityCapital Medical UniversityMinistry of EducationBeijing 100053 China Key Laboratory of Neurodegenerative Diseases Capital Medical UniversityMinistry of EducationBeijing 100053China Department of Neurology Affiliated Hospital of Liaoning Medical UniversityJinzhou 121000Liaoning ProvinceChina

Three cycles of remote ischemic pre-conditioning induced by temporarily occluding the bilateral femoral arteries （10 minutes） prior to 10 minutes of reperfusion were given once a day for 3 days before the animal received middle artery occlusion and reperfusion surgery. The results showed that brain infarct volume was significantly reduced after remote ischemic pre-conditioning. Scores in the forelimb placing test and the postural reflex test were significantly lower in rats having undergone remote ischemic pre-conditioning compared with those who did not receive remote ischemic pre-conditioning. Thus, neurological function was better in rats having undergone remote ischemic pre-conditioning compared with those who did not receive remote ischemic pre-conditioning. These results indicate that remote ischemic pre-conditioning in rat hindlimb exerts protective effects in ischemia-reperfusion injury.

关键词： cerebral ischemia-reperfusion remote ischemic preconditioning stroke neural regeneration

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Effect of remote ischemic preconditioning among donors and recipients following pediatric liver transplantation:A randomized clinical trial

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World Journal of Gastroenterology 2021年第4期27卷 345-357页

作者： Bo Qi Xiao-Qiang Wang Shu-Ting Pan Pei-Ying Li Ling-Ke Chen Qiang Xia Li-Qun Yang Wei-Feng Yu Department of Anesthesiology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Clinical Center for Investigation Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Transplantation and Hepatic Surgery Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200120China

BACKGROUND Studies suggested that remote ischemic preconditioning(RIPC)may effectively lessen the harmful effects of ischemia reperfusion injury during organ transplantation *** To investigate the protective effects of RIPC on living liver donors and recipients following pediatric liver *** From January 2016 to January 2019 at Renji Hospital Affiliated with Shanghai Jiao Tong University School of Medicine,208 donors were recruited and randomly assigned to four groups:S-RIPC group(no intervention;n=55),D-RIPC group(donors received RIPC;n=51),R-RIPC group(recipients received RIPC,n=51)and DR-RIPC group(both donors and recipients received RIPC;n=51).We primarily evaluated postoperative liver function among donors and recipients and incidences of early allograft dysfunction,primary nonfunction and postoperative complications among *** RIPC did not significantly improve alanine transaminase and aspartate aminotransferase levels among donors and recipients or decrease the incidences of early allograft dysfunction,primary nonfunction,and postoperative complications among *** protective effects were observed,including a lower creatinine level in the D-RIPC group than in the S-RIPC group on postoperative day 0(P<0.05).However,no significant improvements were found in donors who received ***,RIPC had no effects on the overall survival of *** The protective effects of RIPC were limited for recipients who received living liver transplantation,and no significant improvement of the prognosis was observed in recipients.

关键词： Pediatric liver transplantation remote ischemic preconditioning Postoperative complications Ischemia reperfusion injury Primary nonfunction Hepatology

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