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Effects of Consecutively Monocultured rehmannia glutinosa *** Diversity of Fungal Community in Rhizospheric Soil

Agricultural Sciences in China 2011年第9期10卷 1374-1384页

作者： ZHANG Zhong-yi LIN Wen-xiong YANG Yan-hui CHEN Hui CHEN Xin-jian Engineering Research Center of Chinese Medicinal Materials Henan Agricultural University Zhengzhou 450002 P.R.China Institute of Agroecology Fujian Agriculture and Forestry University Fuzhou 350002 P.R. China

Continuous monoculture problems, or replanting diseases, are one of the key factors affecting productivity and quality of Chinese medicinal plants. The underlying mechanism is still being explored. Most of the studies on continuous monoculture ofrehmannia glutinosa L. are focused on plant nutritional physiology, root exudate, and its autotoxieity. However, the changes in the diversity of microflora in the rhizosphere mediated by the continuous monoculture pattern have been remained unknown. In this study, terminal restriction fragment length polymorphism （T-RFLP） technique was used for fingerprinting fungal diversity in the rhizosphere soil sampled from the fields ofR. glutinosa monocultured for 1 and 2 yr. The results showed that the structure of fungal community in consecutively moncultured rhizosphere soil was different from that in control soil （no cropping soil）, and varied with the consecutive monoeulture years （1 and 2 yr）. The comprehensive evaluation index （D） of fungal community estimated by principal component analysis of fragment number, peak area, Shannon-Weiner index, and Margalef index was higher in 1 yr monoculture soil than that in 2 yr monoculture soil, suggesting that consecutive monoculture of R. glutinosa could be a causative agent to decrease the diversity of fungal community in the rhizosphere soil.

关键词： biodiversity consecutive monoculture problem fungal community rehmannia glutinosa rhizosphere soil T-RFLP

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A new megastigmane from fresh roots of rehmannia glutinosa

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Acta Pharmaceutica Sinica B 2013年第5期3卷 333-336页

作者： Weisheng Feng Yanyan Lv Xiaoke Zheng Yanli Zhang Yangang Cao Yuanyuan Pei School of Pharmacy Henan University of Traditional Chinese MedicineZhengzhou 450046China

A new megastigmane,rehmamegastigmane(1),together with eighteen known compounds lariciresinol(2),lariciresinol-4′-O-β-D-glucopyranoside(3),hierochin D(4),yemuoside YM1(5),darendoside B(6),decaffeoylacteoside(7),jionoside B1(8),catalpol(9),ajugol(10),6-O-vanilloylajugol(11),6-O-E-feruloylajugol(12),rehmapicroside(13),rehmapicrogenin(14),3-methoxy-2,6,6-trimethylcyclohexane-1-enecarboxylic acid(15),vanillic acid(16),hydroferulic acid(17),threo-1-(4-hydroxy-3-methoxyphenyl)-1,2,3-propanetriol(18),p-hydroxyphenylethyl alcohol(19)was isolated from the fresh roots of rehmannia *** 2–6 and 16–18 were isolated from this plant for the first time.

关键词： Scrophulariaceae rehmannia glutinosa Chemical constituents Iridoids

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Analysis on Pigment and Photosynthetic Characteristics of Leaves of New Strain of rehmannia glutinosa

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Asian Agricultural Research 2017年第6期9卷 101-103,107页

作者： Xing FAN Xiaoting ZHOU Jun WANG Xingchen SHANG Jianjun LI College of Life Sciences Henan Normal University Engineering Laboratory of Biotechnologies for Green Medicinal Plants of HenanProvince

Through the analysis on the leaf color and photosynthetic characteristics of new strains and main cultivars of rehmannia glutinosa,it is expected to provide theoretical basis for breeding of new varieties. Chlorophyll,anthocyanin,and net photosynthetic rate（Pn）,stomatal conductance（Cond）,transpiration rate（Tr）,and intercellular CO2concentration（Ci） in 8 varieties of rehmannia glutinosa were measured by spectrophotometer and LI-6400 XT Portable Photosynthesis System. The results showed that the chlorophyll content of Huaidijin 8（2. 84 mg/g）,Huaidi 81（2. 71 mg/g）,Huaidi 85-5（2. 69 mg/g）,Jinjiu（2. 66 mg/g） and Huaidi 83（2. 63 mg/g） was higher; the anthocyanin content of Jinjiu（0. 169） and Huaidijin 8（0. 165） was higher,while the anthocyanin content Huaidi 83（0. 060） was the lowest; Pn of Huaidi81[2. 41 μmol/（m2·s） ],Huaidi 83[2. 37 μmol/（m2·s） ]and Huaidijin 8[2. 25 μmol/（m2·s） ]was higher,and the anthocyanin content was positively correlated with Pn,while the anthocyanin content was negatively correlated with Pn; Huaidijin 8 and Huaidi 83 showed dominant advantages in single plant fresh weight,indicator component,and resistance over the main cultivars. This indicates that the new variety Huaidijin 8 and Huaidi 83 have excellent comprehensive traits and can be properly popularized.

关键词： rehmannia glutinosa New strains Chlorophyll Anthocyanin Net photosynthetic rate(Pn)

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Cellular basis of Impaired IFN-γ Production after Single Lowdose Total-Body Irradiation and Protection of rehmannia glutinosa

Cellular basis of Impaired IFN-γ Production after Single Lo...

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第十一届全国免疫学学术大会

作者： An Lei Guo Yuqi Sun Xiaoyan Wang Li Zhou Xianbin Lv Yanmin Yao Chengfang Key Laboratory for Tumor Immunology and Traditional Chinese Medicine Immunology Institute of Basic MedicineShandong Academy of Medical Sciences School of Medicine and Life Science University of Jinan-Shandong Academy of Medical Science

Irradiation-induced impaired gamma interferon(IFN-γ) production promotes tumor development and facilitates tumor relapse. Here, we sought to identify the characteristics of IFN-γ-secreting T and NK cells in response to single low-dose total-body irradiation(SLTBI) and also to highlight the role of rehmannia glutinosa(RG) on these cells during the process of recovery from irradiation-induced IFN-γ reduction. All IFN-γ-producing T and NK cells and signaling requirements(MHC-I, MHC-II, CD80,CD86) for Th1/Tc1 proliferation were determined by flow cytometry;the cytokine milieu(IL-12, IL-15, IL-2 etc.) that supports IFN-γ-secreting T and NK cells proliferations and the master transcription factor(T-bet) were detected by RT-PCR assay. The effect of RG on efficient IFN-γ production was confirmed in a lung melanoma metastasis model in irradiated mice. Our data showed that IFN-γ production by T and NK cells was significantly decreased after irradiation. IFN-γ-producing T cells subsets were more sensitive to irradiation injury than NK cells. Decreased nave T cells, reduced signaling from MHC molecular, CD80, CD86 and weakened cytokine support mainly contributed to the severe impaired IFN-γ-secreting T cells(Th1 and Tc1). However, the production of IFN-γ and the transcriptions of IL-12, IL-15, T-bet were obviously increased in RG-treated mice after irradiation(P<0.05). Therapy using RG elicited a remarkable proliferation of Tc1 and NK1 cells, which were necessary for the antitumor effect;and this efficacy of RG was confirmed in lung metastasis tumor model in RG-treated irradiated mice. Taken together, IFN-γ-secreting T cells exhibited a far greater sensitivity to irradiation-induced injury than NK cells. The promotion of RG on cellular basis of IFN-γ production by T and NK cells strengthened the anti-tumor immunity post SLTBI.

关键词： IFN-γ CD4+T CD8+T NK rehmannia glutinosa irradiation

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Photosynthesis of rehmannia glutinosa subjected to drought stress is enhanced by choline chloride through alleviating lipid peroxidation and increasing proline accumulation

Photosynthesis of Rehmannia glutinosa subjected to drought s...

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2006年中国植物逆境生理生态与分子生物学学术研讨会

作者： Huijie Zhao Jinfang Tan Chunmiao Qi (College of Agronomy, Flenan Agricultural University, Zhengzhou 450002, P. R. China )

＜正＞rehmannia glutinosa Libosch, an important herbal plant in Chinese traditional medicine, is widely grown in Northern China because its root tuber has many medical efficacies such as regulating immunity, preventing tumor, reducing blood sugar and pressure, etc. Unfortunately, rehmannia glutinosa seedlings often encounter drought stress during its growth because they are largely grown in the mountainous area. The photosynthesis of rehmannia glutinosa seedlings is frequently inhibited by drought stress and therefore, both yield and medical value of root tuber declined due to the insufficient accumulation of organic matters. Accordingly, it is important to increase the drought tolerance of rehmannia glutinosa seedlings and enhance its photosynthesis in order to improve the yield and quality. The aim of this experiment was to examine the effects of CC on photosynthesis of rehmannia glutinosa seedlings and the related metabolism such as lipid oxidation and proline accumulation under drought stress.

关键词： choline chloride lipid peroxidation proline rehmannia glutinosa drought stress

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Cloning and Sequence Analysis of Actin Gene from rehmannia glutinosa

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Agricultural Science & Technology 2008年第2期9卷 42-44,66页

作者：孙鹏郭玉海祁建军周莉丽李先恩中国农业大学农学与生物技术学院北京100094 中国医学科学院药用植物研究所北京100094

[ Objective ] The aim of this study is to clone and analyze the actin gene from rehmannia glutinosa. [ Method ] Degenerate primers were designed according to the conserved regions of actin sequences of rehmannia glutinosa and its similar species, RT-PCR was next conducted to amplify the actin gene from rehmannia glutinosa. [ Result] The amplified fragment is 724 bp and correspondingly 240 amino acids. The BLAST results indicate that the homology between the amplified fragment and other higher plants for aetin gene sequences and amino acid are more than 80% and 90%, respectively, suggesting that the amplified fragment is the actin gene of rehmannia glutinosa. [ Conclusion] Phylogenetic analysis shows that the actin gene of rehmannia glutinosa has an intimate genetic relationship with actin7 gene of Nicotiana tabacum.

关键词： Actin rehmannia glutinosa Sequence analysis Phylogenetic analysis

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Complete Genome Sequencing and Analysis of rehmannia Mosaic Virus Isolate from Shanxi Province

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Journal of Northeast Agricultural University(English Edition) 2021年第3期28卷 58-65页

作者： Wang De-fu Zhang Xi-mei Guo Shang Shen Shao-fei Long Dan-dan Li Ling-yu Niu Yan-bing College of Life Sciences Shanxi Agricultural UniversityTaigu 030801ShanxiChina Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China Shanxi Institute for Functional Food Shanxi Agricultural UniversityTaiyuan 030031ShanxiChina

Using double-stranded RNA(dsRNA)technology and sequence-independent amplification(SIA),the molecular identification on infected rehmannia glutinosa in the field with mosaic symptoms was performed and the whole-genome of the rehmannia mosaic virus(ReMV)Shanxi isolate(ReMV-SX)was *** analysis showed that the virus that infected rehmannia glutinosa was rehmannia mosaic virus(ReMV).The full-length of the obtained ReMV-SX sequence(GenBank accession ***575184)was 6395 nt,containing four open reading frames(ORFs).The sequence homology analysis of the complete nucleotide sequence showed that ReMV-SX was 93.8%-97.0%homologous to ReMV in Tobamovirus subgroup Ⅰ,while only 49.8%-58.9%homologous to the isolates in subgroups Ⅱ and Ⅲ of the same *** analysis showed that ReMV-SX and ReMV-Henan formed a separate branch and had the closest genetic *** results laid the foundation for ongoing researches in the taxonomic status and evolution of ReMV and for further investigating the pathogenic mechanism of ReMV infecting rehmannia glutinosa.

关键词： rehmannia mosaic virus(ReMV) rehmannia glutinosa whole-genome amplification sequence analysis

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