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Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China

Biomedical and Environmental Sciences 2010年第4期23卷 251-258页

作者： YUAN HU ZHU-JUN SHAO XIAO-MEI YAN BO-QING LI FEI ZHAO DI XIAO JUN REN MING-HUAN ZHENG CHUN-XIANG FAN LI-HUA HE LI XU YI-XIN GU HAI JIANG FENG-HUA GUO ZHEN-WEI DAI HONG-YU REN MEI-JUAN LU XIA CHEN QING-HUA ZOU FAN-LIANG MENG JIAN-ZHONG ZHANG National Institute for Communicable Disease Control and Prevention China CDC P O Box 5 Changping Beijing 102206 China Anhui Provincial Center for Disease Control and Prevention Hefei 230061 Anhui China

Objective During 2003-2005, an outbreak of meningitis due to Neisseria meningitidis serogroup C occurred in China. With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinical isolate, and a carrier strain 053426 with different gene type were analyzed. Methods Clinical strain 053442 and carrier strain 053426 were cultured on GC agar plates under the same condition. Two-dimensional electrophoresis was performed using the pH 3–10 nonlinear IPG strips of 24 cm length, and all the protein spots were identified by matrix-assisted laser desorption/ionization time of flight spectrometry. Results 502 and 380 protein spots were identified in 053426 and 053442 respectively, relating to 266 and 202 different genes covering a wide range of cellular functions. The express volume and number of proteins involved in energy metabolism, protein synthesis and amino acid biosynthesis in 053426 were higher than in 053442. Virulence factor Opa, Opc and a series of proteins involved in pilus assembly and retraction were identified in 053442, which appear to be of primary importance in colonization and invasion of human cells. Compared to 053442, virulence protein species were less in 053426, with lower express volumes too. No Opa and Opc were detected in 053426. Conclusion The different protein expression profiles of the clinical strain 053442 and carrier strain 053426 in the present study provide some clues of the different pathogenicity of the two strains, which may account for result in the final epidemics.

关键词： Neisseria meningitidis reference map Two-dimensional gel electrophoresis MALDI-TOF/TOF-MS

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