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reduning injection versus Neuraminidase Inhibitors in Treatment of Influenza:A Systematic Review and Meta-Analysis

Chinese Journal of Integrative Medicine 2022年第11期28卷 1023-1031页

作者： ZHAO Guo-zhen LI Bo WANG Ya-fan GUO Shi-qi DU Yuan MA Qiu-xiao GUO Yu-hong LIU Qing-quan School of Clinical Medicine Beijing University of Chinese MedicineBeijing(100029)China Department of Emergency Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing(100010)China Beijing Evidence-Based Chinese Medicine Center Beijing(100010)China Beijing Institute of Chinese Medicine Beijing(100010)China School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese MedicineBeijing(100029)China Department of Respiratory Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing(100700)China

Objective:To perform a systematic review to assess the effectiveness and safety of reduning injection versus neuraminidase inhibitors in treatment of ***:The MEDLINE,Embase,the Cochrane Central Register of Controlled Trials(CENTRAL),Chinese Bio-medical Literature and Retrieval System(Sinomed),China National Knowledge Infrastructure Database(CNKI),China Science and Technology Journal Database(VIP),Wanfang Data Knowledge Service Platform and *** were systematically searched from inception dates to May 2021 for randomized controlled trials(RCTs)exploring reduning injection alone or in combination with neuraminidase inhibitors in patients with *** analysis was performed using RevMan 5.4 and Stata *** qualities of the involved studies were assessed by the risk of bias according to the Cochrane *** evidence quality of each outcome was evaluated by GRADEpro ***:Twelve trials with 1,460 patients were *** included studies had a certain unclear or high risk of *** injection appeared to be more effective in shortening the fever clearance time(MD:-16.20 h,95%CI:-19.40 to-12.99,7 trials,814 patients,I^(2)=94%,very low certainty),fever alleviation time(MD:-4.09 h,95%CI:-4.22 to-3.96,3 trials,366 patients,I^(2)=0%,low certainty),cough alleviation time(MD:-21.34 h,95%CI:-41.56 to-1.11,2 trials,228 patients,I^(2)=89%,very low certainty),fatigue alleviation time(MD:-31.83 h,95%CI:-36.88 to-26.77,2 trials,270 patients,I^(2)=0%,low certainty),sore throat alleviation time(MD:-28.66 h,95%CI:-32.23 to-25.10,1 trial,150 patients,low certainty),and improving the total effective rate(RR:1.15,95%CI:1.06 to 1.25,10 trials,1,074 patients,I^(2)=76%,very low certainty).Besides,reduning injection seemed generally ***:This study provided low or very low evidence indicating reduning injection may be effective in the treatment of influenza and might be *** rigorously designed studies are need

关键词： Chinese patent medicine reduning injection influenza meta-analysis systematic review

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reduning injection prevents carrageenan-induced inflammation in rats by serum and urine metabolomics analysis

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Chinese Herbal Medicines 2022年第4期14卷 583-591页

作者： Xia Gao Jiajia Wang Xialin Chen Shanli Wang Chaojie Huang Quanchang Zhang Liang Cao Zhenzhong Wang Wei Xiao Jiangsu Kanion Modern Chinese Medicine Institute Nanjing 210017China State Key Laboratory of Pharmaceutical New-Tech for Chinese Medicine Jiangsu Kanion Pharmaceutical Co.Ltd.Lianyungang 222001China National Enterprise Technology Center National Post-doctoral Research WorkstationJiangsu Enterprise Academician WorkstationLianyungang 222001China China Pharmaceutical University Nanjing 210009China

Objective:To elucidate the anti-inflammatory mechanism of reduning injection(RDN)by analyzing the potential biomarkers and metabolic pathways of the carrageenan-induced inflammatory model from the overall metabolic ***:Rat inflammatory model was established by ***-Q-TOF/MS was used to detect and analyze changes of endogenous metabolites in the serum and urine of carrageenan-induced inflammatory *** with multivariate analysis and databases analysis,inflammatory-related potential biomarkers were screened and identified to analyze possible metabolic *** reliability and biological significance of these biomarkers was verified by metabolic network analysis and correlation analysis with pharmacodynamic ***:A total of 16 potential biomarkers were screened and identified by multivariate analysis and metabolite databases,among which 13 species could be adjusted by *** metabolism pathway analysis revealed that histidine metabolism,sphingolipid metabolism,and tyrosine metabolism were greatly *** biomarkers involved urocanic acid,sphingosine,and norepinephrine,all of which showed a callback trend after RDN *** three biomarkers had a certain correlation with some known inflammatory-related small molecules(histamine,arachidonic acid,Leukotriene B4,and PGE2)and pharmacodynamic indicators(IL-6,IL-1β,PGE2and TNF-a),which indicated that the selected biomarkers had certain reliability and biological ***:RDN has a good regulation of the metabolic disorder of endogenous components in carrageenan-induced inflammatory *** its anti-inflammatory mechanism is mainly related to the regulation of amino acid and lipid *** research method is conducive to the interpretation of the overall pharmacological mechanism of Chinese medicine.

关键词： anti-inflammatory effect metabolomics reduning injection UPLC-Q-TOF/MS

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Active Ingredients of reduning injection Maintain High Potency against SARS-CoV-2 Variants

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Chinese Journal of Integrative Medicine 2023年第3期29卷 205-212页

作者： XIAO Zhen XU Huan QU Ze-yang MA Xin-yuan HUANG Bo-xuan SUN Meng-si WANG Bu-qing WANG Guan-yu Department of Biology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong(518055)China Guangdong Provincial Key Laboratory of Computational Science and Material Design ShenzhenGuangdong Province(518055)China Institute of Chemical Biology Shenzhen Bay LaboratoriesShenzhenGuangdong Province(518132)China State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing(100191)China Institute for Advanced Study Shenzhen UniversityShenzhenGuangdong Province(518052)China Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxiJiangsu Province(214122)China School of Medicine Life and Health Sciences Chinese University of Hong KongShenzhenGuangdong Province(518172)China Center for Endocrinology and Metabolic Diseases Second Affiliated HospitalThe Chinese University of Hong KongShenzhenGuangdong Province(518172)China

Objective:To investigate the anti-coronavirus potential and the corresponding mechanisms of the two ingredients of reduning injection:quercetin and ***:A pseudovirus system was designed to test the efficacy of quercetin and luteolin to inhibit SARS-CoV-2 infection and the corresponding cellular *** was tested for its activities against the pseudoviruses of SARS-CoV-2 and its *** screening was performed to predict the binding sites by Autodock Vina 1.1.230 and *** validate docking results,surface plasmon resonance(SPR)was used to measure the binding affinity of the compounds with various proteins of the *** and luteolin were further tested for their inhibitory effects on other coronaviruses by indirect immunofluorescence assay on rhabdomyosarcoma cells infected with ***:The inhibition of SARS-CoV-2 pseudovirus by luteolin and quercetin were strongly dose-dependent,with concentration for 50%of maximal effect(EC_(50))of 8.817 and 52.98μmol/L,*** cytotoxicity to BHK21-hACE2 were 177.6 and 405.1μmol/L,*** addition,luetolin significantly blocked the entry of 4 pseudoviruses of SARS-CoV-2 variants,with EC50lower than 7μmol/*** screening and SPR confirmed that luteolin binds to the S-proteins and quercetin binds to the active center of the 3CLpro,PLpro,and helicase *** and luteolin showed over 99%inhibition against ***:The mechanisms were revealed of quercetin and luteolin inhibiting the infection of SARS-CoV-2 and its *** injection is a promising drug for COVID-19.

关键词： reduning injection Chinese medicine luteolin quercetin SARS-CoV-2

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reduning injection for fever, rash, and ulcers in children with mild hand, foot, and mouth disease: a randomized controlled clinical study

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Journal of Traditional Chinese Medicine 2013年第6期33卷 733-742页

作者： Guoliang Zhang Jie Zhao Liyun He Shiyan Yan Ziqiang Zhuo Haojie Zheng Yongping Mu Shuangjie Li Xi Zhang Jihan Huang Xiuhui Li Jianping Liu Hehe Wan Chaoyu Wei Wei Xiao Department of Infectious Disease the First Affiliated Hospital of Anhui University of Chinese Medicine Graduate Faculty Anhui University of Chinese Medicine Chinese Medical Clinical Basic Research Institute China Academy of Chinese Medical Sciences Department of Infectious Disease Quanzhou Children's Hospital Department of Combine Traditional Chinese and Western Medicine Department of Combine Traditional Chinese and Western Medicine Shanghai Public Health Centre Department of Infectious Disease Hunan Provincial Children's Hospital Department of Infectious Disease Kaifeng Children's Hospital Drug Clinical Research Center Shanghai University of Chinese Medicine Department of Combine Traditional Chinese and Western Medicine Youan Hospital Evidence Based Medical Center Beijing University of Chinese Medicine Department of Research and Development Jiangsu Kanion Pharmaceutical Llc.

OBJECTIVE： To assess the efficacy and safety of Re- duning injection for fever, rash, and ulcers in chil- dren with mild hand, foot, and mouth disease （HFMD）. METHODS： A stratified-block randomized, dou- ble-blind, parallel-controlled, and multicenter clini- cal trial was conducted with 360 patients in five hospitals across China： Quanzhou Children＇s Hospi- tal, Shijiazhuang No. 5 Hospital, Shanghai Public Health Centre, Hunan Provincial Children＇s Hospi- tal, and Kaifeng Children＇s Hospital. Patients were randomized into three groups with 120 in each. Group A was treated with Western Medicine, group B with reduning injection, a Chinese herbal medi- cine, and group C with both reduning injection and Western Medicine. Results were compared for treatment efficacy and safety on HFMD. The clinical outcomes were observed as follows： fever and on- set time of antifebrile effect （time to bring the body temperature down 〉0.5~C after medication）; cumu- lative time for fever recovery （body temperature re- covering to normal and lasting more than 24 h without medication）; cumulative time for rash dis- appearance （without new rashes or ulcers appear- ing and the original ones fading away）; and cumula- tive time for mouth ulcer disappearance. RESULTS： For the onset time of the antifebrile ef- fect, there was no statistical difference between groups A and B （P〉O.05） and groups B and C （P〉 0.05）. However, there was a statistical difference be- tween groups A and C （P〈0.05）, and the effect ingroup C was the best. For the cumulative time for rash disappearance, there was no statistical differ- ence between groups A and B （P〉0.05）. There were statistical differences between groups A and C, and groups B and C （P〈0.05）, and the effect in group C was the best. For the cumulative time for mouth ul- cers disappearance, there were no statistical differ- ences among the three groups （P〉0.05）. CONCLUSION： reduning injection with Western Medicine for symptomatic treatment is most effec- tive

关键词： Hand, foot and mouth disease Symp-tomatic therapy Randomized controlled trial Treat-ment outcome reduning injection

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A Network Pharmacology Study of reduning injection for the Treatment of Coronavirus Disease-19

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World Journal of Traditional Chinese Medicine 2020年第2期6卷 180-187页

作者： Jian Wu Dong-Dong Ruan Tong-Fei Fu Jun Yuan Clinical College of Traditional Chinese Medicine Hubei University of Chinese Medicine Department of Nephrology Hubei Provincial Hospital of Traditional Chinese MedicineWuhanChina

Objective:The 2019 novel coronavirus(2019-nCoV)outbreak has escalated into a global *** to Chinese guidance for coronavirus disease-19(COVID-19):Prevention,control,diagnosis,and management,reduning injection can effectively treat,the disease caused by the *** identify the active ingredients of reduning injection and COVID-19 disease-related pathways,we conducted a network pharmacology ***:The Traditional Chinese Medicine Systems Pharmacology database was used to screen the chemical constituents and potential targets of reduning *** gene names were converted to the corresponding protein names using Uni *** Cards and OMIM databases were used to select targets related to 2019-n Co *** Cytoscape 3.7.2 software platform and STRING database,we constructed drug-common target and target protein protein-protein interaction network ***643.6.2 software and Bioconductor biological information software package were used for Gene Ontology(GO)functional enrichment and KEGG pathway ***:In reduning injection,a total of 33 effective chemical components were obtained that were involved in 151 signaling pathways,of which 44 targets were considered therapeutically ***:reduning injection can be potentially applied for the treatment of COVID-19 based on the results of our network pharmacology study.

关键词： 2019-novel coronavirus network pharmacology reduning injection signaling pathway target

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A randomized controlled clinical study on treatment of hand, foot and mouth disease in children with reduning injection

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Chinese Medical Journal 2013年第13期126卷 2585-2586页

作者： XU Hua-shan ZHUO Zhi-qiang CHEN Bao-chuan J1NG Jiao ZHAO Guo-rong WU Jun-feng XIAO Wei Quanzhou Children's Hospital Teaching Hospital of Fujian Medical University Quanzhou Fujian 362000 China Jiangsu Kangyuan Pharmaceutical Corporation Ltd. Lianyungang Jiangsu 22200 I China

Hand, foot and mouth disease （HFMD） has become a major public health problem in China since 2008,which has been widely prevalent in children under 5 years of age. According to the data of the Ministry of Health, the number of cases with HFMD exceeded 1 million during the recent 4 years. The major etiologic agent of HFMD is enterovirus 71 and coxsackievirus A 16. The main clinical manifestations of the disease include fever, maculopapular skin vesicles on the hand, foot and buttock, oral herpes, ulcer; ation severe cases there can be central nervous system infection and/or pulmonary hemorrhage, which are mostly caused by EV71 and may be life-threatening with a fatality rate of 0.3 to 0.5 per thousand cases. There are no specific antiviral drugs, nor vaccine against EV71.~ The difficult problem of the treatment is how to reduce the severe cases and mortality. reduning injection is a preparation of traditional Chinese herbal medicine composed of Qinghao （Artemisiae annuae）, Jinyinhua （Flos lonicerae） and Zhizi （Gardenia jasminoides）, which was listed among the therapies for HFMD in the 2010 guidelines of the Ministry of Health for diagnosis and treatment of HFMD.2 We now reported the results of our study on the efficacy of reduning injection in treatment of 81 patients with HFMD. We selected 81 cases who were inpatients treated in 2010 in the Department of Infectious Diseases of Quanzhou Chidren＇s Hospital. The inclusion criteria were as follows： 1. Patients who met either the clinical diagnostic criteria for HFMD ordinary clinical type or the clinical diagnostic criteria for confirmed cases of HFMD （etiologically confirmed cases must account for more than 1/3 of the cases）. 2. The axillary temperature is _〉37.5~C, within 48 hours after onset of the disease; 3. Skin changes such as the rashes and vesicles occurred in no more than 48 hours; 4. Range of age is between 1 and 13 years; 5. Patients or their parents or guardians are willing to participate in the study and si

关键词： hand foot and mouth disease reduning injection children

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Anti-Inflammatory Effects of reduning injection(热毒宁注射液) on Lipopolysaccharide-Induced Acute Lung Injury of Rats

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Chinese Journal of Integrative Medicine 2014年第8期20卷 591-599页

作者：唐陆平萧伟李怡芳李海波王振中姚新生 Hiroshi Kurihara 何蓉蓉 Pharmacy College Jinan University State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process

Objective： To evaluate the protective effects of reduning injection （热毒宁注射液, RDN）, a patent Chinese medicine, on lipopolysaccharide （LPS）-induced acute lung injury （ALl） in rats and its underlying mechanisms of action. Methods： Sixty male Sprague-Dawley rats were randomly divided into 6 groups, including normal control, model, dexamethasone （DEX, 5 mg/kg）, RDN-H （720 mg/kg）, RDN-M （360 mg/kg） and RDN-L （180 mg/kg） groups, with 10 rats in each group. Rats were challenged with intravenous injection of LPS 1 h after intraperitoneal treatment with RDN or DEX. At 6 h after LPS challenge, lung tissues and bronchoalveolar lavage fluid （BALF） were collected, and the number of inflammatory cells was determined. The right lungs were collected for histopathologic examination, measurement of gene and protein expressions, superoxide dismutase （SOD） and myeloperoxidase （MPO） activities. Results： In vivo pretreatment of RDN （360, 720 mg/kg） significantly reduced the weight of wet to dry （W/D） ratio of lung, protein content in BALF, and led to remarkable attenuation of LPS-induced histopathological changes in the lungs. Meanwhile, RDN enormously decreased BALF total inflammatory cells, especially neutrophil and macrophage cell numbers. Moreover, RDN increased SOD activity, inhibited MPO activity, alleviated LPS-induced tumor neurosis factor-o~ （TNF-o~） and inducible nitric oxide synthase （iNOS） expression in lung tissues. Furthermore, RDN （720 mg/kg） efficiently weakened nuclear factor- kappa B （NF- K B） gene and protein expression. Conclusion： Anti-inflammatory effects of RDN was demonstrated to be preventing pulmonary neutrophil infiltration, lowering MPO activity, TNF-oL and iNOS gene expression by inhibiting NF- K B activity in LPS-induced ALl.

关键词： acute lung injury lipopolysaccharide neutrophils chemokine nuclear factor-kappa B reduning injection Chinese patent medicine

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Network pharmacology studies on reduning injection in treatment of COVID-19

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Asian Toxicology Research 2020年第3期2卷 85-96页

作者： Zhi-Hua Yang Yi-Hua Fan Yi-Fei Ma Ping Xin Yuan-Yuan Liu Lin Wang Hai-Feng Yan Ting-Ting Lv First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300381China The Fourth Clinical Medical College of Xinjiang Medical University Urumqi 830000China Tianjin University of Traditional Chinese Medicine Tianjin301617China Jiangsu College of Nursing Huai’an 223005China

Background:Network pharmacology was used to explore the mechanism of the Chinese patent medicine reduning injection in the treatment of corona virus disease ***:The chemical constituents and targets of Qinghao(Artemisiae annuae herba),Jinyinhua(Lonicerae japonicae flos),Zhizi(Gardeniae fructus)in the Chinese patent medicine reduning injection were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,and the target related to corona virus disease 2019 was searched in GeneCards database,then perform Venn analysis on the targets of corona virus disease 2019 and the Chinese patent medicine reduning injection,to screen the compounds and targets of the Chinese patent medicine reduning injection in the treatment of corona virus disease *** String platform was used to construct the protein-protein interaction network,and key targets were *** 3.5.1 was used to construct the active traditional Chinese medicine-chemical composition-target-disease network to screen the key active components,and the gene ontology function and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed on the common target through DAVID(6.8)online analysis data tool to predict the mechanism of ***:Fifty-two active ingredients and 232 targets were selected from the Chinese patent medicine reduning injection,including 44 targets related to corona virus disease 2019.A total of 310 gene ontology biological processes and 94 Kyoto Encyclopedia of Genes and Genomes signaling pathways were obtained,which were mainly involved in inflammation,viral infection,bacterial infection,immune response and substance metabolism,et ***:The mechanism of the Chinese patent medicine reduning injection in the treatment of corona virus disease 2019 may be related to antivirus,bacteriostasis,anti-inflammatory and antifebrile,immune regulation and metabolism regulation,et al.

关键词： Network pharmacology SARS-CoV-2 reduning injection COVID-19

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Meta-analysis of the comparison between reduning injection and ribavirin injection for acute upper respiratory tract infection in children

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Infectious Diseases Research 2022年第4期3卷 1-9页

作者： Chen Yang Lin-Hui Li Shi-Pian Li Shi-Jin Cheng Tian-Yu Zhu Wei Zhai Tianjin University of Traditional Chinese Medicine Tianjin 301617China

Objective:To systematically evaluate the effectiveness and safety of reduning injection in acute upper respiratory tract infection of ***:Use computer to search PubMed,the Cochrane Library,EMBASE,Web of Science,China National Knowledge Infrastructure(CNKI),Wanfang Database,Weipu database(VIP)and China Biomedical studies Service System(CBM)for randomized controlled trials(RCT)of acute upper respiratory tract infection in children,and the retrieval time is from their establishment to May,*** two researchers independently screened the studies,extracted data and evaluated the risk bias of studies,RevMan5.3 software was used to perform ***:10 studies in total were included,involving 1466 *** of the meta-analysis:total effective rate[RR=1.12,95%CI(1.07,1.16),Preduning injection is more effective than ribavirin injection in clinical,and it is better than ribavirin injection at relieving fever,nasal obstruction and nose running,cou

关键词： acute upper respiratory tract infection reduning injection ribavirin injection meta-analysis

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Two new terpenoids from reduning injection

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Chinese Herbal Medicines 2020年第2期12卷 183-187页

作者： Hai-bo Li Biao Yang Wen Ge Ze-yu Cao Liang Cao Yang Yu Zhen-zhong Wang Xin-sheng Yao Wei Xiao Jiangsu Kanion Pharmaceutical Co. Ltd.Lianyungang 222001China State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process Lianyungang 222001China Zhejiang Pharmaceutical College Ningbo 315000China Institute of Traditional Chinese Medicine&Natural Products Guangdong Key Laboratory of Pharmacodynamic Constituents of TCM and New Drug ResearchCollege of PharmacyJinan UniversityGuangzhou 510632China

Objective:To study the antipyretic and anti-inflammatory constituents from the active fraction of reduning(RDN)***:In this study,the active fraction of RDN injection was screened by the LPS-induced mouse endotoxin shock *** chemical constituents were isolated by chromatography on HP-20 macroporous adsorptive resins,silica gel,ODS columns and reverse phase MPLC and HPLC repeatedly,and their structures were elucidated based on spectroscopic analysis(HR-ESI-MS,NMR,ECD)and chemical ***,we evaluated the anti-inflammatory activities of the isolates by measuring their inhibitory effects on TNF-αproduction in LPS stimulated RAW 264.7 ***:The 95%ethanol eluate of RDN injection by the macroporous adsorption resin column was proved to be the antipyretic and anti-inflammatory active fraction of this injection.A novel iridoid,named jasminoide A(1),and a new guaiane sesquiterpenoid,named(1 R,7 R,8 S,10 R)-7,8,11-trihydroxy-4-guaien-3-one(2),were isolated from reduning injection,and compound 2 can inhibit TNF-αproduction with IC50 values of 72.24μmol/***:In this study,two new terpenoids were isolated from reduning injection,and compound 2 showed inhibitory activity against LPS-activated TNF-αproduction in RAW 264.7 cells in vitro.

关键词： (1R,7R,8S,10R)-7,8,11-trihydroxy-4-guaien-3-one anti-inflammation jasminoide A reduning injection TNF-α

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