检索结果-南通市图书馆

Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides

引用

Synthetic and Systems Biotechnology 2021年第4期6卷 335-342页

作者： Tong Shi Lu Zhang Mindong Liang Weishan Wang Kefeng Wang Yue Jiang Jing Liu Xinwei He Zhiheng Yang Haihong Chen Chuan Li Dongyuan Lv Liming Zhou Biqin Chen Dan Li Li-Xin Zhang Gao-Yi Tan State Key Laboratory of Bioreactor Engineering(SKLBE) And School of BiotechnologyEast China University of Science and Technology(ECUST)Shanghai200237China State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)Beijing100101China Inner Mongolia Kingdomway Pharmaceutical Co. LtdHohhot010206China

The versatile photosyntheticα-proteobacterium Rhodobacter sphaeroides,has recently been extensively engineered as a novel microbial cell factory(MCF)to produce pharmaceuticals,nutraceuticals,commodity chemicals and even ***,there are no well-characterized high-activity promoters to modulate gene transcription during the engineering of *** this study,several native promoters from *** JDW-710(JDW-710),an industrial strain producing high levels of co-enzyme Q10(Q10)were selected on the basis of transcriptomic *** candidate promoters were then characterized by using gusA as a reporter *** native promoters,Prsp_7571 and Prsp_6124,showed 620%and 800%higher activity,respectively,than the tac promoter,which has previously been used for gene overexpression in *** addition,a Prsp_7571-derived synthetic promoter library with strengths ranging from 54%to 3200%of that of the tac promoter,was created on the basis of visualization of red fluorescent protein(RFP)expression in ***,as a demonstration,the synthetic pathway of Q10 was modulated by the selected promoter T334*in JDW-710;the Q10 yield in shake-flasks increased 28%and the production reached 226 mg/*** well-characterized promoters should be highly useful in current synthetic biology platforms for refactoring the biosynthetic pathway in ***-derived MCFs.

关键词： Rhodobacter sphaeroides Promoter library Transcriptomics Co-enzyme Q_(10) red fluorescent protein

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Production of Transgenic Pig Clone Embryo Expressed the red fluorescent protein by Using the Somatic Cell Nucleus Transplantation Technology

引用

Agricultural Science & Technology 2011年第2期12卷 287-290,307页

作者：康锦丹尹熙俊赵明辉梁爽刘希李文学崔成哲延边大学动物胚胎工程研究所吉林延吉133002 延边大学农学院吉林延吉133002 延边农业科学研究院吉林龙井133400

[Objective] The research aimed to lay the foundation for producing the transgenic clone pig.[Method] The pig fetus fibroblast with the red fluorescent protein（RFP）gene that was transfected by the retrovirus was as the donor of nucleus *** using the somatic cell cloning technology,the development situation in vitro of clone embryo with RFP was studied.[Result] The fusion rate of RFP transgenic cell was 83.87% which had no significant difference with 80.56% of non-transgenic cell（P0.05）.The blastula rate in vitro of RFP transgenic somatic cell reconstructed embryo was 8.67% which had no significant difference with 6.56% of non-transgenic cell（P0.05）.After the reconstructed embryo of RFP transgenic somatic cell was transplanted into fifteen receptors,there was no conception individual.[Conclusion] The transgenic cell with the red fluorescent protein as the donor could successfully clone the transgenic embryo and obtain the transgenic blastula.

关键词： Transgene red fluorescent protein Nucleus transplantation Pig

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Construction of Expression Vector Capable of Excising Selectable Marker Gene

引用

Agricultural Biotechnology 2013年第1期2卷 33-37页

作者： Hewei WANG Limin WANG Ping ZHOU Yinguo ZHANG College of Animal Science and Technology Shihezi University The Breed & Biotechnology Key Laboratory of Sheep Xinjiang Production & Construction GroupXinjiang Academy of Agricultural Reclamation Sciences

[ Objective] This study was to construct an expression vector capable of excising selectable marker gene, further eliminating the effect of marker gene on the functional study of target gene. [ Method] By using Cre/LoxP site-specific recombination system, Dsred2-1 vector was modified by introducing LoxP se- quence and multiple cloning site sequence, then the TK gene was ligated into this vector for negative selection. [ Results] The fragments introduced were recom- bined at the molecular level under induced condition, and the specific red fluorescence was also observed in the recombinant vector transfected cells at the cellular level. [ Conclusion] It is feasible to use this Cre/loxP system to excise marker genes, showing a broad application prospect.

关键词： Cre/loxP recombination system Selecmble marker genes red fluorescent protein Excision

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

rfp基因两种遗传转化方法导入荧光假单胞菌的比较分析（英文）

rfp基因两种遗传转化方法导入荧光假单胞菌的比较分析（英文）

引用

作者：贾艳华宋福平李国勋黄大昉张杰中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室莱阳农学院植保系中国农业科学院生物技术研究所中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室

＜正＞Eukaryotic fluorescent proteins are being used increasingly as bacterial markers in microbial e-cology, particularly for monitoring genetically engineered bacteria in environment. red fluorescent protein （RFP） expressed in bacteria can be detected under either daylight or UV/blue light. In this study, two marker systems were constructed. The rfp gene was introduced into the Pseudomonas fluorescens strain P303 by a multi-copies shuttle plasmid vector and integrated into the chromosome via single copy mini-Tn5 transposon, yielding respectively, the recombinant 12P303 and the conjugant 8’ P303. Sodium dodecyl sulfate polyacryamide gel electrophoresis （SDS-PAGE） showed that the quantity of RFP expressed in the multi-copies recombinant 12P303 was greater than that in the single copy conjugant 8’ P303. The copy number of the marker gene was very important but decisive. The stability of the marked rfp gene in P303 was outstanding, and the beneficial anti - fungal qualities of the host strain P303 were not affected by expression of the foreign gene rfp. The test of the marked 12P303 and 8’ P303 in the soil of a greenhouse indicated that the marked strains were safe for the environment and did not affect the indigenous bacteria in the soil. As a marker gene for P. ftuorescens, the rfp gene exceeds the gfp gene in a number of ways. A simple and sensitive tool, the confocal scanning laser microscope （CSLM）, should be considered for the detection of marked microorganisms. Construction of two different marker systems provides researchers with a wide-range of potential applications for engineered microorganisms.

关键词： red fluorescent protein Pseudomonas fluorescens Mini-Th5 transposon shuttle plasmid vector

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：