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3H-31, A Non-structural Protein of Heliothis virescens ascovirus 3h,Inhibits the Host Larval Cathepsin and Chitinase Activities

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Virologica Sinica 2021年第5期36卷 1036-1051页

作者： Huan Yu Yi-Yi Ou-Yang Chang-Jin Yang Ni Li Madoka Nakai Guo-Hua Huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangsha 410128China College of Plant Protection Hunan Agricultural UniversityChangsha 410128China Tokyo University of Agriculture and Technology SaiwaiFuchuTokyo 183-8509Japan

3h-31 of Heliothis virescens ascovirus 3h(Hv AV-3h)is a highly conserved gene of *** an early gene of Hv AV-3h,3h-31 codes for a non-structural protein(3H-31)of Hv *** the study,3h-31 was initially transcribed and expressed at 3 h post-infection(hpi)in the infected Spodoptera exigua fat body cells(Se FB).3h-31 was further inserted into the bacmid of Autographa californica nucleopolyhedrovirus(Ac MNPV)to generate an infectious baculovirus(Ac MNPV-31).In vivo experiments showed that budded virus production and viral DNA replication decreased with the expression of 3H-31,and lucent tubular structures were found around the virogenic stroma in the Ac MNPV-31-infected Se FB *** vivo,both LD50and LD90values of Ac MNPV-31 were significantly higher than those of the wild-type Ac MNPV(Ac MNPV-wt)in third instar *** *** interesting finding was that the liquefaction of the larvae killed by the infection of Ac MNPV-31 was *** and cathepsin activities of Ac MNPV-31-infected larvae were significantly lower than those of Ac MNPV-wt-infected *** possible regulatory function of the chitinase and cathepsin for 3H-31 was further confirmed by RNAi,which showed that larval cathepsin activity was significantly upregulated,but chitinase activity was not significantly changed due to the RNAi of *** on the obtained results,we assumed that the function of 3H-31 was associated with the inhibition of host larval chitinase and cathepsin activities,so as to restrain the hosts in their larval stages.

关键词： Cathepsin Chitinase Heliothis virescens ascovirus 3h(HvAV-3h) recombinant virus 3h-31

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